Volume 14, Issue 12 (12-2016)                   IJRM 2016, 14(12): 737-742 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Davari-tanha F, Shahrokh Tehraninejad E, Ghazi M, Shahraki Z. The role of G-CSF in recurrent implantation failure: A randomized double blind placebo control trial. IJRM 2016; 14 (12) :737-742
URL: http://ijrm.ir/article-1-717-en.html
1- Department of Reproductive Endocrinology, Women Hospital, Vali-e-Asr Health Reproductive Center, Tehran University of Medical Sciences, Tehran, Iran , fatedavtanha@gmail.com
2- Department of Reproductive Endocrinology, Vali-e-Asr Hospital, Vali-e-Asr Health Reproductive Center, Tehran University of Medical Sciences, Tehran, Iran
3- Department of Reproductive Endocrinology, Women Hospital, Vali-e-Asr Health Reproductive Center, Tehran University of Medical Sciences, Tehran, Iran
Abstract:   (2740 Views)
Background: Recurrent implantation failure (RIF) is the absence of implantationafter three consecutive In Vitro Fertilization (IVF) cycles with transferring at leastfour good quality embryos in a minimum of three fresh or frozen cycles in a womanunder 40 years. The definition and management of RIF is under constant scrutiny.
Objective: To investigate the effects of Granulocyte colony stimulating factor (GCSF)on RIF, pregnancy rate, abortion rate and implantation rates.
Materials and Methods: A double blind placebo controlled randomized trial wasconducted at two tertiary university based hospitals. One hundred patients with thehistory of RIF from December 2011 until January 2014 were recruited in the study.G-CSF 300μg/1ml was administered at the day of oocyte puncture or day ofprogesterone administration of FET cycle. Forty patients were recruited at G-CSFgroup, 40 in saline and 20 in placebo group.
Results: The mean age for whole study group was 35.3±4.2 yrs (G-CSF 35.5±4.32,saline 35.3±3.98, placebo 35.4±4.01, respectively). Seventeen patients had a positivepregnancy test after embryo transfer [10 (25%) in G-CSF; 5 (12.5%) in saline; and 2(10%) in placebo group]. The mean of abortion rates was 17.6% (3), two of them inG-CSF, one in saline group. The implantation rate was 12.3% in G-CSF, 6.1% insaline and 4.7% in placebo group.
Conclusion: G-CSF may increase chemical pregnancy and implantation rate inpatients with recurrent implantation failure but clinical pregnancy rate and abortionrate was unaffected.
Full-Text [PDF 334 kb]   (565 Downloads) |   |   Full-Text (HTML)  (405 Views)  
Type of Study: Original Article |

References
1. Coughlan C, Ledger W, Wang Q, Liu F, Demirol A, Gurgan T, et al. Recurrent implantation failure: Definition and Management. Reprod Biomed Online 2014; 28: 14-38. [DOI:10.1016/j.rbmo.2013.08.011]
2. Polanski LT, Baumgarten MN, Quenby S, Brosens J, Campbell BR, Raine-Fenning NJ. What exactly do we mean by 'recurrent implantation failure'? A systematic review and opinion. Reprod Biomed Online 2014; 28: 409-423. [DOI:10.1016/j.rbmo.2013.12.006]
3. Blake D, Farquhar C, Johnson N, Proctor M. Cleavage stage versus blastocyst stage embryo transfer in assisted conception. Cochrane Database Syst Rev 2007; CD002118.
4. Ferraretti AP, La Marca A, Fauser BC, Tarlatzis B, Nargund G, Gianaroli L. ESHRE consensus on the definition of 'poor response' to ovarian stimulation for in vitro fertilization: the Bologna criteria. Hum Reprod 2011; 26: 1616-1624. [DOI:10.1093/humrep/der092]
5. Rotterdam ESHRE/ASRM–Sponsored PCOS Consensus Workshop Group, 2004. Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome. Fertil Steril 2004; 81: 19-25.
6. Taylor H. The role of HOX genes in human implantation. Hum Reprod Update 2000; 6: 75-79. [DOI:10.1093/humupd/6.1.75]
7. Taylor E, Gomel V. The uterus and fertility. Fertil Steril 2008; 89: 1-16. [DOI:10.1016/j.fertnstert.2007.09.069]
8. Shokeir T, El-Shafei M, Yousef H, Allam AF, Sadek E. Submucous myomas and their implications in the pregnancy rates of patients with otherwise unexplained primary infertility undergoing hysteroscopic myomectomy: a randomized matched control study. Fertil Steril 2010; 94: 724-729. [DOI:10.1016/j.fertnstert.2009.03.075]
9. Bosteels J, Weyers S, Puttemans P, Panayotidis C, Van Herendael B, Gomel V, et al. The effectiveness of hysteroscopy in improving pregnancy rates in subfertile women without other gynaecological symptoms: a systematic review. Hum Reprod Update 2010; 16: 1-11. [DOI:10.1093/humupd/dmp033]
10. Varasteh NN, Neuwirth RS, Levin B, Keltz MD. Pregnancy rates after hysteroscopic polypectomy and myomectomy in infertile women. Obstet Gynecol 1999; 94: 168-171.
11. Tremellen K, Russell P. Adenomyosis is a potential cause of recurrent implantation failure during IVF treatment. Aust N Z J Obstet Gynaecol 2011; 51: 280-283. [DOI:10.1111/j.1479-828X.2010.01276.x]
12. Thebault N, Broux PL, Moy L, Vialard J. Utilization of Essure micro-insert for hydrosalpinx occlusion in infertile women. J Gynecol Obstet Biol Reprod 2012; 41: 145-150.
13. Cutting R, Morroll D, Roberts SA, Pickering S, Rutherford A. Elective single embryo transfer: guidelines for practice British Fertility Society and Association of Clinical Embryologists. Hum Fertil (Camb) 2008; 11: 131-146. [DOI:10.1080/14647270802302629]
14. Yang YS, Melinda S, Ho HN, Hwang JL, Chen SU, Lin HR, et al. Effect of the number and depth of embryos transferred and unilateral or bilateral transfer in tubal embryo transfer (TET). J Assist Reprod Genet 1992; 9: 534-538. [DOI:10.1007/BF01204250]
15. Weissman A, Horowitz E, Ravhon A, Nahum H, Golan A, Levran D. Pregnancies and livebirths following ICSI with testicular spermatozoa after repeated implantation failure using ejaculated spermatozoa. Reprod Biomed Online 2008; 17: 605-609. [DOI:10.1016/S1472-6483(10)60306-9]
16. Thomas J, Liu F, Link DC. Mechanisms of mobilization of hematopoietic progenitors with granulocyte colony-stimulating factor. Curr Opin Hematol 2002; 9: 183-189. [DOI:10.1097/00062752-200205000-00002]
17. Litwin S, Lagadari M, Barrientos G, Roux ME, Margni R, Miranda S. Comparative immunohistochemical study of M-CSF and G-CSF in feto-maternal interface in a multiparity mouse model. Am J Reprod Immunol 2005; 54: 311-320. [DOI:10.1111/j.1600-0897.2005.00317.x]
18. Marino VJ, Roguin LP. The granulocyte colony stimulating factor (G-CSF) activates Jak/STAT and MAPK pathways in a trophoblastic cell line. J Cell Biochem 2008; 103: 1512-1523. [DOI:10.1002/jcb.21542]
19. McCracken S, Layton JE, Shorter SC, Starkey PM, Barlow DH, Mardon HJ. Expression of granulocyte-colony stimulating factor and its receptor is regulated during the development of the human placenta. J Endocrinol 1996; 149: 249-258. [DOI:10.1677/joe.0.1490249]
20. McCracken SA, Grant KE, MacKenzie IZ, Redman CW, Mardon HJ. Gestational regulation of granulocyte-colony stimulating factor receptor expression in the human placenta. Biol Reprod 1999; 60: 790-796. [DOI:10.1095/biolreprod60.4.790]
21. Yarali H, Bukulmez O. The effect of intramural and subserous uterine fibroids on implantation and clinical pregnancy rates in patients having intracytoplasmic sperm injection. Arch Gynecol Obstet 2002; 266: 30-33. [DOI:10.1007/PL00007491]
22. Yasmin H, Nasir A, Noorani KJ. Hystroscopic management of Ashermans syndrome. J Pak Med Assoc 2007; 57: 553-555.
23. Yu D, Wong YM, Cheong Y, Xia E, Li TC. Asherman syndrome-one century later. Fertil Steril 2008; 89: 759-779. [DOI:10.1016/j.fertnstert.2008.02.096]
24. Ayida G, Chamberlain P, Barlow D, Kennedy S. Uterine cavity assessment prior to in-vitro fertilization: comparison of transvaginal scanning, saline contrast hysterosonography and hysteroscopy. Ultrasound Obstet Gynecol 1997; 10: 59-62. [DOI:10.1046/j.1469-0705.1997.10010059.x]
25. Daiter E, Pampfer S, Yeung YG, Barad D, Stanley ER, Pollard JW. Expression of colony-stimulating factor-1 in the human uterus and placenta. J Clin Endocrinol Metab 1992; 74: 850-858. [DOI:10.1210/jcem.74.4.1548350]
26. Pampfer S, Daiter E, Barad D, Pollard JW. Expression of the colonystimulating factor-1 receptor (c-fms proto-oncogene product) in the human uterus and placenta. Biol Reprod 1992; 46: 48-57. [DOI:10.1095/biolreprod46.1.48]
27. Saito S, Fukunaga R, Ichijo M, Nagata S. Expression of granulocyte colonystimulating factor and its receptor at the fetomaternal interface in murine and human pregnancy. Growth Factors 1994; 10: 135-143. [DOI:10.3109/08977199409010987]
28. Kauma SW, Aukerman SL, Eierman D, Turner T. Colony-stimulating factor-1 and c-fms expression in human endometrial tissues and placenta during the menstrual cycle and early pregnancy. J Clin Endocrinol Metab 1991; 73: 746-751. [DOI:10.1210/jcem-73-4-746]
29. Scarpellini F, Sbracia M. Use of granulocyte colony-stimulating factor for the treatment of unexplained recurrent miscarriage: a randomised controlled trial. Hum Reprod 2009; 24: 2703-2708. [DOI:10.1093/humrep/dep240]
30. Barash A, Dekel N, Fieldust S, Segal I, Schechtman E, Granot I. Local injury to the endometrium doubles the incidence of successful pregnancies in patients undergoing in vitro fertilization. Fertil Steril 2003; 79: 1317-1322. [DOI:10.1016/S0015-0282(03)00345-5]
31. Zhou L, Li R, Wang R, Huang HX, Zhong K. Local injury to the endometrium in controlled ovarian hyperstimulation cycles improves implantation rates. Fertil Steril 2008; 89: 1166-1176. [DOI:10.1016/j.fertnstert.2007.05.064]
32. Würfel W, Santjohanser C, Hirv K, Bühl M, Meri O, Laubert I, et al. High pregnancy rates with administration of granulocyte colony-stimulating factor in ART-patients with repetitive implantation failure and lacking killer-cell immunoglobulin-like receptors. Hum Reprod 2010; 25: 2151-2152. [DOI:10.1093/humrep/deq106]
33. Rahmati M, Petitbarat M, Dubanchet S, Bensussan A, Chaouat G, Ledee N. Granulocyte-Colony Stimulating Factor Related Pathways Tested on an Endometrial Ex-Vivo Model. PloS One 2014; 9: e102286. [DOI:10.1371/journal.pone.0102286]
34. Santjohanser C, Knieper C, Franz C, Hirv K, Meri O, Schleyer M, et al. Granulocyte-colony stimulating factor as treatment option in patients with recurrent miscarriage. Arch Immunol Ther Exp (Warsz) 2013; 61: 159-164. [DOI:10.1007/s00005-012-0212-z]
35. Gleicher N, Vidali A, Barad DH. Successful treatment of unresponsive thin endometrium. Fertil Steril 2011; 95: 13-17. [DOI:10.1016/j.fertnstert.2011.01.143]
36. Gleicher N, Kim A, Michaeli T, Lee HJ, Shohat-Tal A, Lazzaroni E, et al. A pilot cohort study of granulocyte colony-stimulating factor in the treatment of unresponsive thin endometrium resistant to standard therapies. Hum Reprod 2013; 28: 172- 177. [DOI:10.1093/humrep/des370]
37. Kunicki M, Łukaszuk K, Woclawek-Potocka I, Liss J, Kulwikowska P, Szczyptańska J. Evaluation of granulocyte colony-stimulating factor effects on treatment-resistant thin endometrium in women undergoing in vitro fertilization. Biomed Res Int 2014: 913235.
38. Li Y, Pan P, Chen X, Li L, Li Y, Yang D. Granulocyte colony-stimulating factor administration for infertile women with thin endometrium in frozen embryo transfer program. Reprod Sci 2014; 21: 381-385. [DOI:10.1177/1933719113497286]
39. Tehraninejad E, Davari Tanha F, Asadi E, Kamali K, Aziminikoo E, Rezayof E. G-CSF Intrauterine for Thin Endometrium, and Pregnancy Outcome. J Fam Reprod Health 2015; 9: 107-112.
40. Barad DH, Yu Y, Kushnir VA, Shohat-Tal A, Lazzaroni E, Lee HJ, et al. A randomized clinical trial of endometrial perfusion with granulocyte colony-stimulating factor in in vitro fertilization cycles: impact on endometrial thickness and clinical pregnancy rates. Fertil Steril 2014; 101: 710-717. [DOI:10.1016/j.fertnstert.2013.12.016]
41. Scarpellini F, Sbracia M. Use of granulocyte colony-stimulating factor for the treatment of unexplained recurrent miscarriage: a randomised controlled trial. Hum Reprod 2009; 24: 2703-2708. [DOI:10.1093/humrep/dep240]
42. Bildirici I, Bukulmez O, Ensari A, Yarali H, Gurgan T. A prospective evaluation of the effect of salpingectomy on endometrial receptivity in cases of women with communicating hydrosalpinges. Hum Reprod 2001; 16: 2422-2426. [DOI:10.1093/humrep/16.11.2422]
43. Genbacev O, Prakobphol A, Foulk R, Krtolica A, Ilic D, et al. Trophoblast L-selectin-mediated adhesion at the maternal-fetal interface. Science 2003; 299: 405-408. [DOI:10.1126/science.1079546]

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb