Volume 5, Issue 3 (7-2007)                   IJRM 2007, 5(3): 41-0 | Back to browse issues page

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Nayernia K, Lee J H, Engel W, Nolte J, Drusenheimer N, Rathsack K, et al et al . From stem cells to germ cells and from germ cells to stem cells. IJRM 2007; 5 (3) :41-0
URL: http://ijrm.ir/article-1-74-en.html
From stem cells to germ cells and from germ cells to stem cells
Karim Nayernia * 1, Jae Ho Lee2 , Wolfgang Engel3 , Jessica Nolte3 , Nadja Drusenheimer3 , Kristina Rathsack3 , Arvind Dev3 , Gerald Wulf4 , Ingrid E Ehrmann2 , David Elliott2 , Ulrich Zechner5 , Thomas Haaf5 , Andreas Meinhardt6 , Hans W Michelmann7 , Gerlad Hasenfuss8 , Kaomei Guan8
1- North East Institute of Stem Cell Biology, Institute of Human Genetics, International Centre for Life, Central Parkway, University of Newcastle upon Tyne, UK , karim.nayernia@newcastle.ac.uk
2- North East Institute of Stem Cell Biology, Institute of Human Genetics, International Centre for Life, Central Parkway, University of Newcastle upon Tyne, UK
3- Institute of Human Genetics, University of Goettingen, Germany
4- Department of Haematology and Oncology, University of Göttingen, 37073 Göttingen, Germany
5- Institute of Human Genetics, University of Mainz, 51131 Mainz, Germany
6- Department of Anatomy and Cell Biology, University of Giessen, 35378 Giessen, Department of Anatomy and Cell Biology, University of Giessen, 35378 Giessen, Germany
7- Department of Obstetrics and Gynecology, University of Göttingen, 37073 Göttingen, Germany
8- Department of Cardiology, University of Goettingen, Germany
Abstract:   (3568 Views)
Germline and somatic stem cells are distinct types of stem cells that are dedicated to reproduction and somatic tissue regeneration, respectively. Germline stem cells (GSCs), which can self-renew and generate gametes, are unique stem cells in that they are solely dedicated to transmit genetic information from generation to generation. We developed a strategy for the establishment of germline stem cell lines from embryonic stem cells (ES). These cells are able to undergo meiosis, generate haploid male gametes in vitro and are functional, as shown by fertilization after intra-cytoplasmic injection into mouse oocytes. In other approach, we show that bone marrow stem (BMS) cells are able to trans-differentiate into male germ cells. BMS cell-derived germ cells expressed the known molecular markers of primordial germ cells. The ability to derive male germ cells from ES and BMS cells reveals novel aspects of germ cell development and opens the possibilities for use of these cells in reproductive medicine. Conversely, we showed that adult male germline stem cells, spermatogonial stem cells (SSCs), can be converted into embryonic stem cell like cells which can differentiate into the somatic stem cells of three germ layers. Understanding how SSC can give rise to pluripotent stem cells and how somatic stem cells differentiate into germ cells could give significant insights into the regulation of developmental totipotency as well as having important implications for male fertility and regenerative medicine.
Keywords: Stem cells, Germ cells, Bone Marrow stem cells, Spermatognial stem cells
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Type of Study: Original Article |
References
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