Volume 5, Issue 4 (7-2007)                   IJRM 2007, 5(4): 121-126 | Back to browse issues page

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Peirouvi T, Farjah G, Soleimani Rad J, Ghaffari Novin M. Vitrification induced apoptosis in spermatozoa from fertile and subfertile men. IJRM 2007; 5 (4) :121-126
URL: http://ijrm.ir/article-1-79-en.html
1- Department of Histology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran , tpeirouvi@yahoo.co.uk
2- Department of Histology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran
3- Depatment of Histology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz , Iran
4- Department of Reproductive Endocrinology and Embryology, Avesina Research Center, Shahid Beheshti University, Tehran, Iran
Abstract:   (2451 Views)
Background: Phospholipids are distributed asymmetrically between inner and outer leaflets of the plasma membrane of live cells. Early during apoptosis, this asymmetry is disrupted and phosphatidylserine becomes exposed on the outside surface of the plasma membrane. There is little information about the effects of vitrification on apoptosis.
Objective: The aim of the present study was to evaluate the effect of vitrification on apoptosis of subfertile and fertile men.
Materials and Methods: In this study, semen samples were collected from subfertile (n=20) and fertile men (n=10) after 48h abstinence of intercourse. After semen analysis according to WHO criterias, each semen sample was divided into two portions. First portion was assessed by Annexin V-flous staining kit for showing apoptosis in subfertile and fertile men and second portion was assessed after vitrification-thawing. Results were analyzed by Paired t-test and Independent t-test.
Results: After vitrification-thawing, mean percentage of apoptotic spermatozoa has increased 6 and 3 times in subfertile and fertile men respectively. This difference is significant.
Conclusion: Vitrification-thawing could disrupted membrane asymmetry and caused apoptosis. Therefore, it will cause reduction of functional spermatozoa in access of Assisted Reproduction Technologies (ART).
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Type of Study: Original Article |

References
1. Sherman LK. Freezing and freeze-drying of human spermatozoa. Fertil Steril 1954;5:357-371. [DOI:10.1016/S0015-0282(16)31685-5]
2. Giraud M, Motta C, Boucher D, Grizard G: Membrane fluidity predicts the outcome of cryopreservation of human spermatozoa. Hum Reprod 2000; 15:2160-2164. [DOI:10.1093/humrep/15.10.2160]
3. Donnelly ET, Steele EK, McClure N, Lewis SE. Assessment of DNA integrity and morphology of ejaculated spermatozoa from fertile and infertile men before and after cryopreservation. Hum Reprod 2001; 16:1191-1199. [DOI:10.1093/humrep/16.6.1191]
4. Centola GM, Raubertas RF, Mattox JH: Cryopreservation of humansemen: comparison of cryopreservation, sources of variability, and prediction of post-thaw survival. J Androl 1992; 13:283-288.
5. Duru NK, Morshedi M, Schuffner A, Oehninger S. Cryopreservation-thawing of fractionated human spermatozoa and plasma membrane translocation of phosphatidylserine. Fertil Steril 2001; 75:263-268. [DOI:10.1016/S0015-0282(00)01694-0]
6. Schuffner A, Morshedi M, Oehninger S. Cryopreservation of fractionated, highly motile human spermatozoa: Effect on membrane phosphatidylserine externalization and lipid peroxidation. Hum Reprod 2001; 16:2148-2153. [DOI:10.1093/humrep/16.10.2148]
7. Paasch U, Sharma RK, Gupta KA, Grunewald S, Mascha EJ, Thomas AJ, et al: Cryopreservation and thawing is associated with varying extent of activation of apoptotic machinery in subsets of ejaculated human spermatozoa. Biol Reprod 2004; 71:1828-1837. [DOI:10.1095/biolreprod.103.025627]
8. Vayena E, Rowe PJ, Griffin PD. Report of a WHO meeting Current practices and controversies in assisted reproduction. WHO, Geneva 2002; 156.
9. Ortiz A, Cuadrado SG, Lorz C, Egido J. Apoptosis in renal diseases. Front Biosci 1996; 1:30-47. [DOI:10.2741/A114]
10. Nagata S. Apoptosis by death factor. Cell 1997; 88:355-365. [DOI:10.1016/S0092-8674(00)81874-7]
11. Glander HJ, Schaller J. Binding of annexin V to plasma membranes of human spermatozoa: A rapid assay for detection of membrane changes after cryostorage. Mol Hum Reprod 1999 ; 5:109-115. [DOI:10.1093/molehr/5.2.109]
12. Schwartz LM, Ashwell JD: Apoptosis. Volume 66, USA, Academic press, 2001; 33-84
13. Shen HM, Dai J, Chia SE, Lim A, Ong CN: Detection of apoptotic alterations in sperm in subfertile patients and their correlations with sperm quality. Hum Reprod 2002;17:1266-1273. [DOI:10.1093/humrep/17.5.1266]
14. World Health Organization (WHO) laboratory manual for the examination of human semen and semen-servical mucus interaction. 4th ed, Cambridge :Cambridge University Press;1999: 6-23.
15. Vermes I, Haanen C, Steffens-Nakken H, Reutelinqsperger C: A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J Immunol Methods 1995;184:39-51. [DOI:10.1016/0022-1759(95)00072-I]
16. Barroso G, Morshedi M, Oehninger S: Analysis of DNA fragmentation, plasma membrane translocation of phosphatidylserine and oxidative stress in human spermatozoa. Hum Reprod 2000; 15:1338-1344. [DOI:10.1093/humrep/15.6.1338]
17. Alhasani S, Ozmen B, Kouttaki N, Schoepper B, Diedrich K, Schultze-Mosgau A: Three years of routin vitrification of human zygotes: is still fair to advocate slow rate freezing? Biomedicine Online 2007; 14: 288-293. [DOI:10.1016/S1472-6483(10)60869-3]

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