Volume 1, Issue 1 (1-2003)                   IJRM 2003, 1(1): 7-11 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Khalili M A, G Rabchevsky A. Restoration of Spermatogenesis by Adenoviral Gene Transfer into Injured Spinal Cords of Rats. IJRM 2003; 1 (1) :7-11
URL: http://ijrm.ir/article-1-8-en.html
Restoration of Spermatogenesis by Adenoviral Gene Transfer into Injured Spinal Cords of Rats
Mohammad A Khalili * , Alexander G Rabchevsky
Abstract:   (2503 Views)
Materials and Methods: Young adult Sprague-Dawley rats (200-250g) were assigned into one of the three different groups of control, SCI, and adenovirus transfer (Ad) (n=3/ group). Control rats received no injury, nor any surgery. For SCI rats, SCI was produced by a 10g brass rod with a tip diameter of 2 mm which was dropped from a height of 12.5 mm onto exposed spinal cord at level of T10 with NYU impactor. Animals were perfused transcardially 43 days post SCI. Both spinal cord and testicular tissues were cryo-sectioned and ultra thin-sectioned, respectively. Cellular morphology and morphometry were done for spinal cord tissues. The testicular samples were processed for both light and transmission electron microscopy (TEM). The third group of rats underwent SCI first, followed by microinjection of LacZ adenoviral vectors (5x106 p.f.u./ �l) along the T6-T10 dorsal root entry zone bilaterally. The immune system of animals were suppressed before the Ad administration. Each Ad injection was done using a glass micropipet and a Nonoject injector. Rats were killed 43 days after Ad injections, and the tissues were studied as for other groups.
Keywords: Spinal cord injury, Gene therapy, Spermatogenesis, Rat
Full-Text [PDF 445 kb]   (522 Downloads) |   |   Full-Text (HTML)  (333 Views)  
Type of Study: Original Article |
References
1. Bors E, Comarr E. Neurological disturbances of sexual function with speical reference to 529 patients with spinal cord injury. Urol Surv 1960; 10: 191-222.
2. Hirsch IH, Huang B, Chancellor MB, Rivas DA, Salzman SK, Jost LK, Evenson DP. Spermatogenesis in early and chronic phases of experimental spinal cord injury in the rodent model. J Androl 1999; 20: 63-71.
3. Holstein AE, Saverwein D, Schirren U. Spermatogenesis in patients with traumatic transverse paralysis. Urologe 1985; 24: 208-11.
4. Linsenmeyer TA, Perkash I. Infertility in men with spinal cord injury. Arch Phys Med Rehabil 1991; 72: 747-54.
5. Linsenmeyer TA, Pogach L, Ottenweller JE, Huang HF. Spermatogenesis and the pituitary testicular hormone axis in rats during acute phase of spinal cord injury. J Urol 1994; 152: 1303-7. [DOI:10.1016/S0022-5347(17)32572-7]
6. Liu Y, Himes BT, Moul J, Huang W, Chow S, Tessler A, Fischer I. Application of recombinant adenovirus for in vivo gene delivery to spinal cord. Brain Research 1997; 768: 19-29. [DOI:10.1016/S0006-8993(97)00587-8]
7. Rabchevsky AG, Fugaccia I, Turner AF, Blades DA, Mattson MP, Scheff SW. Basic fibroblast growth factors enhances functional recovery following severe spinal cord injury to the rat. Exp Neurol 2000; 164: 280-91. [DOI:10.1006/exnr.2000.7399] [PMID]
8. Rajasekaran M, Monga M. Cellular and molecular causes of male infertility in spinal cord injury. J Androl 1999; 20: 326-30.
9. Robbins PD, Ghivizzani SC. Viral vectors for gene therapy. Pharmacol Ther 1998; 80: 35-47. [DOI:10.1016/S0163-7258(98)00020-5]
10. Romero MI, Smith GM. Adenoviral gene transfer into the normal and injured spinal cord: enhanced transgene stability by combined administration of temperature-sensitive virus and transient immune blockade. Gene Therapy 1998; 5: 1612-21. [DOI:10.1038/sj.gt.3300774] [PMID]
11. Stribley JM, Rehman KS, Niu H, Christman GM. Gene therapy and reproductive medicine. Fert Steril 2002; 77: 645-57. [DOI:10.1016/S0015-0282(01)03233-2]
12. Wickham Tj. Targeting adenovirus. Gene Therapy 2000; 7: 110-14. [DOI:10.1038/sj.gt.3301115] [PMID]
13. Wood M, Charlton H, Wood K, Kajiwara K, Byrnes A. Immune responses to adenovirus vectors in the nervous system. Trends Neurosci 1996; 19; 497-501. [DOI:10.1016/S0166-2236(96)10060-6]
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb