Volume 15, Issue 5 (6-2017)                   IJRM 2017, 15(5): 279-286 | Back to browse issues page

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Sabeti P, Amidi F, Kalantar S M, Sedighi Gilani M A, Pourmasumi S, Najafi A et al . Evaluation of intracellular anion superoxide level, heat shock protein A2 and protamine positive spermatozoa percentages in teratoasthenozoospermia. IJRM 2017; 15 (5) :279-286
URL: http://ijrm.ir/article-1-824-en.html
Evaluation of intracellular anion superoxide level, heat shock protein A2 and protamine positive spermatozoa percentages in teratoasthenozoospermia
Parvin Sabeti1 , Fardin Amidi2 , Seyed Mahdi Kalantar3 , Mohammad Ali Sedighi Gilani4 , Soheila Pourmasumi3 , Atefeh Najafi2 , Ali Reza Talebi * 5
1- Department of Anatomy, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
2- Department Of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
3- Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
4- Department of Urology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
5- Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , prof_talebi@ssu.ac.ir
Abstract:   (2946 Views)
Background: Teratoasthenozoospermia (TA) is a severe form of male infertilitywith no clear etiology.
Objective: To compare the level of intracellular anion superoxide (O2–), heat shockprotein A2 (HSPA2) and protamine deficiencies in ejaculated spermatozoa betweenteratoasthenozoospermic and normozoospermic men.
Materials and Methods: In this case- control study, semen samples of 20 infertilemen, with TA (with normal morphology lower than 4%_ and total motility lowerthan 40% ) as the case group and 20 normozoospermic fertile men as the controlgroup were evaluated for intracellular O2– and HSPA2 by flow cytometry andprotamine deficiency by Chromomycin A3 (CMA3) test.
Results: The rate of CMA3+ spermatozoa in the case group was higher thancontrols (p=0.001). The percentages of HSPA2+ spermatozoa in the cases weresignificantly lower than controls (p=0.001). Also, intracellular O2– levels in the casegroup were significantly higher than controls (p=0.001) and had positivecorrelations with sperm apoptosis (r=0.79, p=0.01) and CMA3 positive sperm(r=0.76, p=0.01), but negative correlations with normal morphology (r=-0.81,p=0.01) and motility (r=-0.81, p=0.01). There was no significant correlation betweenintracellular O2– and HSPA2 in the case group (r=0.041, p=0.79).
Conclusion: We suggest that the increase in intracellular O2–, decrease inspermatozoa HSPA2+, and high percentages of spermatozoa with immaturechromatin might be considered as etiologies of infertility in TA patients.
Keywords: Male infertility, Sperm chromatin, HSPA2, Protamine deficiency.
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Type of Study: Original Article |
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