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Reproductive Biomedicine
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Volume 15, Issue 7 (8-2017)
IJRM 2017, 15(7): 413-422 |
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Mohammadzadeh E, Amjadi F, Movahedin M, Zandieh Z, Nazmara Z, Eslahi N, et al . In vitro development of embryos from experimentally Kerack-addicted Mice. IJRM 2017; 15 (7) :413-422
URL: http://ijrm.ir/article-1-843-en.html
URL: http://ijrm.ir/article-1-843-en.html
Elham Mohammadzadeh1 
, Fatemeh-Sadat Amjadi2 , Mansoureh Movahedin3 , Zahra Zandieh4 , Zohreh Nazmara4 , Neda Eslahi3 , Peymaneh Shirinbayan5 , Hamid Reza Asgari2 , Nahid Azad6 , Maryam Salimi6 , Morteza Koruji * 7
, Fatemeh-Sadat Amjadi2 , Mansoureh Movahedin3 , Zahra Zandieh4 , Zohreh Nazmara4 , Neda Eslahi3 , Peymaneh Shirinbayan5 , Hamid Reza Asgari2 , Nahid Azad6 , Maryam Salimi6 , Morteza Koruji * 7
1- Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran
2- Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran
3- Anatomical Sciences Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran
4- Department of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
5- Pediatric Neuro-Rehabilitation Research Center, the University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.
6- Department of Reproductive Biology and Anatomical Sciences, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
7- Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran , koruji.m@iums.ac.ir
2- Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran
3- Anatomical Sciences Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran
4- Department of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
5- Pediatric Neuro-Rehabilitation Research Center, the University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.
6- Department of Reproductive Biology and Anatomical Sciences, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
7- Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran , koruji.m@iums.ac.ir
Abstract: (3003 Views)
Background: Prenatal drug exposure, as a common public health concern, is associated with an increased risk of adverse effects on early embryo development.
Objective: To investigate the in vitro development of - embryo from experimentally Kerack-addicted mice.
Materials and Methods: Twenty-five female mice were studied in five groups: control, vehicle, and three experimental groups of Kerack-dependent mice (I, II, and III) which received different doses of Kerack for 14 days. After the establishment of addiction model (7 days), experimental groups I, II, and III were given Kerack intraperitoneally at the doses of 5, 35, and 70 mg/kg, twice a day for a period of 7 days, respectively. The vehicle group received normal saline and lemon juice whilst the control group just received water and food. Morulae were obtained through oviduct flashing. The survived embryos were cultured in T6+ 5mg/ml bovine serum albumin. The developmental rates up to hatched stage daily and embryo quality (differential staining and Tunnel staining) were also assessed
Results: The developmental potential of embryos obtained from the addicted mother was significantly decreased in comparison with control group. There was a significant reduction in the rate of blastocyst formation in the high dose Kerack dependent group. However, in addicted mice there was reduction in the total cell number (40.92% vs. 65.08% in control) and, inner cell mass percentage (17.17% vs. 26.15% in control) while apoptotic cells numbers were increased (7.17 vs. 1.46 in control) (p<0.05).
Conclusion: The Kerack addiction during pregnancy retards preimplantation development and induces apoptosis.
Objective: To investigate the in vitro development of - embryo from experimentally Kerack-addicted mice.
Materials and Methods: Twenty-five female mice were studied in five groups: control, vehicle, and three experimental groups of Kerack-dependent mice (I, II, and III) which received different doses of Kerack for 14 days. After the establishment of addiction model (7 days), experimental groups I, II, and III were given Kerack intraperitoneally at the doses of 5, 35, and 70 mg/kg, twice a day for a period of 7 days, respectively. The vehicle group received normal saline and lemon juice whilst the control group just received water and food. Morulae were obtained through oviduct flashing. The survived embryos were cultured in T6+ 5mg/ml bovine serum albumin. The developmental rates up to hatched stage daily and embryo quality (differential staining and Tunnel staining) were also assessed
Results: The developmental potential of embryos obtained from the addicted mother was significantly decreased in comparison with control group. There was a significant reduction in the rate of blastocyst formation in the high dose Kerack dependent group. However, in addicted mice there was reduction in the total cell number (40.92% vs. 65.08% in control) and, inner cell mass percentage (17.17% vs. 26.15% in control) while apoptotic cells numbers were increased (7.17 vs. 1.46 in control) (p<0.05).
Conclusion: The Kerack addiction during pregnancy retards preimplantation development and induces apoptosis.
Type of Study: Original Article |
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