شنبه 8 دی 1403
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دوره 5، شماره 5 - ( 4-1386 )
جلد 5 شماره 5 صفحات 181-177 |
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Mostajeran F, Naderi M, Adibi S. Effects of melatonin on histopathological changes after experimental ovarian torsion-detorsion in cat. IJRM 2007; 5 (5) :177-181
URL: http://ijrm.ir/article-1-88-fa.html
URL: http://ijrm.ir/article-1-88-fa.html
Effects of melatonin on histopathological changes after experimental ovarian torsion-detorsion in cat. International Journal of Reproductive BioMedicine. 1386; 5 (5) :177-181
چکیده: (2215 مشاهده)
Background: During the detorsion of a torsioned ovary, oxidant agents are released and melatonin as an antioxidant can reduce ischemia. We studied the histopathological changes after using melatonin on experimental torsioned ovary in cat.
Objective: The aim of this experimental study was to investigate the effects of Melatonin on histopathological changes in torsion – detorsion injury in cat ovary.
Materials and Methods: An adnexal torsion – detorsion model was created by using 20 adult cats randomly divided equally in to 2 groups of Saline and Melatonin. Ischemia was induced by iathrogenic 360° clockwise torsion of the cat adnex for 3 hr. Reperfusion was achieved for 3 hr. Melatonin or saline were injected intra peritoneally (10mg/kg) 30 min before ovarian detorsion in both groups. After 3 hr of ovarian detorsion, ovarian tissue was removed and fixed in 10% formalin solution, embedded in paraffin and evaluated for ischemic indices.
Results: Histological examination showed a significant improvement in ovarian morphology in the melatonin treated cats. Edema and vasoconstriction in saline group were more severe than Melatonin group (p-value = 0.009). Hemorrhage and leukocyte infiltration were also more obvious in saline group (p-value 0.0018)
Conclusion: Our results demonstrated that Melatonin administration reduced ovarian histopathological damage due to oxidative injury associated with torsion.
Objective: The aim of this experimental study was to investigate the effects of Melatonin on histopathological changes in torsion – detorsion injury in cat ovary.
Materials and Methods: An adnexal torsion – detorsion model was created by using 20 adult cats randomly divided equally in to 2 groups of Saline and Melatonin. Ischemia was induced by iathrogenic 360° clockwise torsion of the cat adnex for 3 hr. Reperfusion was achieved for 3 hr. Melatonin or saline were injected intra peritoneally (10mg/kg) 30 min before ovarian detorsion in both groups. After 3 hr of ovarian detorsion, ovarian tissue was removed and fixed in 10% formalin solution, embedded in paraffin and evaluated for ischemic indices.
Results: Histological examination showed a significant improvement in ovarian morphology in the melatonin treated cats. Edema and vasoconstriction in saline group were more severe than Melatonin group (p-value = 0.009). Hemorrhage and leukocyte infiltration were also more obvious in saline group (p-value 0.0018)
Conclusion: Our results demonstrated that Melatonin administration reduced ovarian histopathological damage due to oxidative injury associated with torsion.
نوع مطالعه: Original Article |
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