Volume 6, Issue 5 (7-2008)                   IJRM 2008, 6(5): 199-204 | Back to browse issues page

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Javed A, Rezaei-Zarchi S, Anvari M, Javeed Ghani M, Barzegari Firouzabadi F, Jamil A, et al . Determination of effective dosage of FSH and hCG in the maturation of preantral follicles and enclosed oocytes in mice. IJRM 2008; 6 (5) :199-204
URL: http://ijrm.ir/article-1-124-en.html
Determination of effective dosage of FSH and hCG in the maturation of preantral follicles and enclosed oocytes in mice
Aisha Javed1 , Saeed Rezaei-Zarchi2 , Morteza Anvari1 , Madiha Javeed Ghani3 , Fatemeh Barzegari Firouzabadi2 , Amer Jamil4 , Seyed Mehdi Kalantar * 5, Habibollah Nazem2
1- Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2- Department of Biology, Payam-e-Noor University, Yazd, Iran
3- Department of Bioinformatics, Government College University, Faisalabad, Pakistan
4- Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan
5- Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , smkalantar@yahoo.com
Abstract:   (2922 Views)
Background: In vitro maturation (IVM) of oocytes reduces the costs and averts the side-effects of gonadotropin stimulation for in vitro fertilization (IVF). Reliable IVM is an intellectual, scientific and clinical challenge with a number of potential applications.
Objective: The effect of hCG was evaluated on the timing and regulation of in vitro ovulation for the Syrian mice oocytes in the presence and absence of FSH. 
Materials and Methods: Preantral follicles, isolated from the ovaries of 6 weeks-old mice, were cultured in TCM-199 medium. The effect of 10-200 mIU/ml FSH and 1.5 IU/ml hCG was seen on the follicle maturation, as well as the changes in ovulation capacity of enclosed oocytes, after the incubation period of 6 days at 37 °C, 92% humidity and 5% CO2 in air.
Results: 100 mIU/ml FSH showed increased follicle diameter, survival, germinal vesicle breakdown (GVBD) and oocyte maturation rates (p<0.0001). Significantly higher number of follicles showed cumulus attachment when ovulation started within 16-24 hours post hCG (97% and 80% respectively; p<0.0001) as compared to the cultures without hCG or when the ovulation time increased from 24 hours post hCG. Combination of FSH and hCG showed 97% (p<0.0001) ovulation as compared to that seen for FSH-containing medium only (81%) or control (10%).
Conclusion: The combined administration of 1.5 IU/ml hCG and 100 mIU/ml FSH induces the in vitro follicle maturation, ovulation capacity and proper timing of mice oocytes. 
Keywords: Follicle stimulating hormone, HCG, Preantral follicles, Oocyte maturation, GVBD.
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Type of Study: Original Article |
References
1. Weon-Young S, Jin-Tae C, Ri-Cheng C, Belen H, Ezgi D, Shai E, et al. 38 h interval between hCG priming and oocyte retrieval increases in vivo and in vitro oocyte maturation rate in programmed IVM cycles. Human Reprod 2008; 23: 2010-2016. [DOI:10.1093/humrep/den210]
2. Chian RC, Buckett WM, Tulandi T, Tan SL. Prospective randomized study of human chorionic gonadotrophin priming before immature oocyte retrieval from unstimulated women with polycystic ovarian syndrome. Hum Reprod 2000; 15: 165-170. [DOI:10.1093/humrep/15.1.165]
3. Lin YH, Hwang JL, Huang LW, Mu SC, Seow KM, Chung J, et al. Combination of FSH priming and hCG priming for in-vitro maturation of human oocytes. Hum Reprod 2003; 18: 1632-1636. [DOI:10.1093/humrep/deg335]
4. Son WY, Lee SY, Yoon SH, Lim JH. Pregnancies and deliveries after transfer of human blastocysts derived from in vitro matured oocytes in in vitro maturation cycles. Fertil Steril 2007; 87: 1491-1493. [DOI:10.1016/j.fertnstert.2006.11.027]
5. Smitz JEJ, Cortvrindt GR. The earliest stages of folliculogenesis in vitro. Reprod 2002; 123: 185-202. [DOI:10.1530/rep.0.1230185]
6. O'Brien MJ, Pendola JK, Eppig JJ. A revised protocol for in vitro development of mouse oocytes from primordial follicles dramatically improves their developmental competence. Biol Reprod 2003; 68: 1682-1686. [DOI:10.1095/biolreprod.102.013029]
7. Liu X, Andoh K, Mizunuma H, Kamijo T, Kikuchi N, Yamada K, et al. Effects of recombinant human FSH (rhFSH), urinary purified FSH (uFSH) and hMG on small preantral follicles and tertiary follicles from normal adult and androgen-sterilized female mice. Fertil Steril 2000; 73: 372-380. [DOI:10.1016/S0015-0282(99)00494-X]
8. Abel MH, Wootton AN, Wilkins V, Huhtaniemi I, Knight PG, Charlton HM. The effect of a null mutation in the follicle-stimulating hormone receptor gene on mouse reproduction. Endocrinol 2000; 141: 1795-1803. [DOI:10.1210/endo.141.5.7456]
9. Wang Y, Rippstein PU, Tsang BK. Role and gonadotrophic regulation of X-linked inhibitor of apoptosis protein expression during rat ovarian follicular development in vitro. Biol Reprod 2003; 68: 610-619. [DOI:10.1095/biolreprod.102.007807]
10. Tan SL, Child TJ, Gulekli B. In vitro maturation and fertilization of oocytes from unstimulated ovaries: predicting the number of immature oocytes retrieved by early follicular phase ultrasonography. Am J Obstet Gynecol 2002; 186: 684-689. [DOI:10.1067/mob.2002.122146]
11. Son WY, Yoon SH, Lee SW, Ko Y, Yoon HG, Lim JH. Blastocyst development and pregnancies after IVF of mature oocytes retrieved from un-stimulated patients with PCOS after in-vivo HCG priming: case report. Hum Reprod 2002; 17: 134-136. [DOI:10.1093/humrep/17.1.134]
12. Mikkelsen AL, Lindenberg S. Influence of the dominant follicle on in-vitro maturation of human oocytes: a prospective non-randomized study. RBM Online 2001; 3: 199-204. [DOI:10.1016/S1472-6483(10)62036-6]
13. Conti M. Specificity of the cyclic adenosine 3', 5'-monophosphate signal in granulosa cell function. Biol Reprod 2002; 67: 1653-1661. [DOI:10.1095/biolreprod.102.004952]
14. Mahmoudi R, Subhani A, Pasbakhsh, Abolhasani F, Amiri I, Salehnia M, et al. The Effects of cumulus cells on in vitro maturation of mouse germinal vesicle stage oocytes. Ir J Reprod Med 2005; 3: 74-78.
15. Javed A, Jamil A, Rezaei-Zarchi S, Anvari M, Nazem H, Kalantar SM. An in vitro comparative study of follicle stimulating hormone (FSH) and activin A effects on the maturation of preantral follicle-enclosed oocytes from immature Syrian mice. Ir Biomed J 2008; 12: 85-92.
16. Javed A, Rezaei-Zarchi S, Jamil A, Kalantar SM. An in vitro comparative study of the effects of ascorbic acid and FSH on the maturation of Syrian mice follicles. Mid East Fretil Soc J 2008; 13: 135-142.
17. Mitchell LM, Kennedy CR, Hartshorne GM. Effects of varying gonadotrophin dose and timing on antrum formation and ovulation efficiency of mouse follicles in vitro. Hum Reprod 2002; 17: 1181-1188. [DOI:10.1093/humrep/17.5.1181]
18. Bishonga C, Takashi Y, Katagiri S, Nagano M, Ishikawa A. In vitro growth of ovarian peantral follicles and the capacity of their oocytes to develop to the blastocyst stage. J Vet Med Sci 2001; 63: 619-624. [DOI:10.1292/jvms.63.619]
19. Albertini DF, Combelles CM, Benecchi E, Carabatsos MJ. Cellular basis for paracrine regulation of ovarian follicle development. Reprod 2001; 121: 647-653. [DOI:10.1530/rep.0.1210647]
20. Mao J, Wu G, Smith MF, McCauley MC, Cantley TC, Prather RS, et al. Effects of culture medium, serum type, and various concentrations of follicle-stimulating hormone on porcine preantral follicular development and antrum formation in vitro. Biol Reprod 2002; 67: 1197-1203. [DOI:10.1095/biolreprod67.4.1197]
21. Demeestere I, Centner J, Gervy C, Englert Y, Delbaere A. Impact of various endocrine and paracrine factors on in vitro culture of preantral follicles in rodents. Reprod 2005; 130: 147-156. [DOI:10.1530/rep.1.00648]
22. Cecconi S, Rossi G, Coticchio G, Macchiarelli G, Borini A, Canipari R. Influence of thyroid hormone on mouse preantral follicle development in vitro. Fertil Steril 2004; 81: 919-924. [DOI:10.1016/j.fertnstert.2003.11.014]
23. Demeestere I, Gervy C, Centner J, Devreker F, Englert Y, Delbaere A. Effect of insulin-like growth factor-I during preantral follicular culture on steroidogenesis, in vitro oocyte maturation and embryo development in mice. Biol Reprod 2004; 70: 1664-1669. [DOI:10.1095/biolreprod.103.023317]
24. Marilyn JO, Janice KP, John J. A revised protocol for in vitro development of mouse oocytes from primordial follicles dramatically improves their developmental competence. Biol Reprod 2003; 68: 1682-1686. [DOI:10.1095/biolreprod.102.013029]
25. Hreinsson J, Bjoern R, Barbro F, Lev L, Ingvar E, Anne-Maria S, et al. Recombinant LH is equally effective as recombinant hCG in promoting oocyte maturation in a clinical in-vitro maturation programme: a randomized study. Human Reprod 2003; 18: 2131-2136. [DOI:10.1093/humrep/deg422]
26. Erickson GF, Shimasaki S. The physiology of folliculogenesis: the role of novel growth factors. Fertil Steril 2001; 76: 943-949. [DOI:10.1016/S0015-0282(01)02859-X]
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