Volume 16, Issue 9 (September 2018)                   IJRM 2018, 16(9): 571-576 | Back to browse issues page

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Mirabutalebi S H, Karami N, Ashrafzadeh H R, Akhvansales Z, Tavakoli M, Ghasemi N. Detection of 4977-bp deletion of mitochondrial DNA in in vitro fertilization failure women: A case-control study. IJRM 2018; 16 (9) :571-576
URL: http://ijrm.ir/article-1-1241-en.html
Detection of 4977-bp deletion of mitochondrial DNA in in vitro fertilization failure women: A case-control study
Seyed Hamidreza Mirabutalebi1 , Noorodin Karami1 , Hamid Reza Ashrafzadeh2 , Zhima Akhvansales3 , Maryam Tavakoli4 , Nasrin Ghasemi * 5
1- Genetics Department, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2- Abortion Research Centre, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
3- Biotechnology Research Centre, International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
4- Research and Clinical Center for infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
5- Abortion Research Centre, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , ghasemi@ssu.ac.ir
Abstract:   (2968 Views)
Background: The quality of oocyte is often considered as a limiting factor for fertility, especially IVF. Some mitochondrial mutations, particularly the 4977-bp deletion increase with the age. Thus, this mutation can serve as a marker for cell aging, which indicates the reduced quality of the oocytes for fertilization. It has been suggested that this can also be investigated in the blood cells of women with IVF failure.
Objective: 1-Determination of the frequency of 4977-bp deletion in women with IVF failure, 2-Investigation of the relationship between 4977-bp deletion and the age of patients.
Materials and Methods: Polymerase chain reaction was used to detect the 4977-bp deletion in blood samples of 52 IVF failure women and 52 women who had at least one healthy child. After polymerase chain reaction with deleted and wild-type primers, the products were examined using agarose gel electrophoresis.
Results: 48.07% of women with IVF failure and 34.62% of healthy women had a mitochondrial 4977-bp deletion, with p=0.163 and OR: 1.749. Also, in association with the age of these patients and the frequency of 4977-bp mutation, p and OR were obtained 0.163 and 1.749, respectively and frequency of this mutation was higher in patients over 35 yr old compared to other subgroups (Patients ≥35: 57.69).
Conclusion: According to the findings of this study, there is no a significant relationship between the frequency of mitochondrial 4977-bp mutation and failure in IVF.
Keywords: In vitro fertilization, Mitochondria, Mutation, Cell aging
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Type of Study: Original Article |
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