Volume 18, Issue 9 (September 2020)                   IJRM 2020, 18(9): 701-712 | Back to browse issues page

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Kasraeian M, Ghasemi E, Dianatpour M, Tanideh N, Razeghian Jahromi I, Khodabandeh Z, et al . In utero xenotransplantation of mice bone marrow-derived stromal/stem cells into fetal rat liver: An experimental study. IJRM 2020; 18 (9) :701-712
URL: http://ijrm.ir/article-1-1468-en.html
In utero xenotransplantation of mice bone marrow-derived stromal/stem cells into fetal rat liver: An experimental study
Maryam Kasraeian1 , Elahe Ghasemi1 , Mehdi Dianatpour2 , Nader Tanideh3 , Iman Razeghian Jahromi4 , Zahra Khodabandeh5 , Mohammad Reza Dorvash5 , Shahrokh Zare5 , Omid Koohi Hosseinabadi6 , Amin Tamadon * 7
1- Department of Obstetrics and Gynecology, Maternal-Fetal Medicine Research Center, Perinatology Ward, Shiraz University of Medical Sciences, Shiraz, Iran.
2- Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Department of Human Genetic, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
3- Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
4- Cardiovascular Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
5- Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
6- Central Lab, Shiraz University of Medical Sciences, Shiraz, Iran.
7- The Persian Gulf Marine Biotechnology Research Center, the Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran. , amintamaddon@yahoo.com
Abstract:   (2070 Views)
Background: Animals can play an important role in preparing tissues for human through the development of xenotransplantation protocols. The most common problem with liver transplantation like any other organ transplantation is organ supply shortage.
Objective: To evaluate the in utero xenotransplantation of mouse bone marrow-derived stromal/stem cells (BMSCs) to the liver of rat fetus to produce mouse liver tissue.
Materials and Methods: BMSCs were isolated and confirmed from enhanced green fluorescent protein (eGFP)-genetic labeled mice. Using a microinjection protocol, mice BMSCs were injected into the liver of rat fetuses in utero on day 14 of pregnancy. After birth, livers were collected and the presence of mice eGFP-positive cells in rat livers was evaluated through polymerase chain reaction.
Results: The eGFP mRNA was detected in the liver of injected infant rats. BMSCs of adult mice were capable to remain functional probably as hepatocyte-like cells in liver of infant rats after in utero xenotransplantation.
Conclusion: BMSCs have the potential for intrauterine xenotransplantation for the treatment of liver dysfunction before birth. This method can also be used for xenoproduction of liver tissue for transplantation.
Keywords: Xenotransplantation, Liver, Bone marrow, Stromal/stem cell, Murine.
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Type of Study: Original Article | Subject: Stem Cell & Cloning
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