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Volume 18, Issue 8 (August 2020)
IJRM 2020, 18(8): 571-578 |
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Tahtamouni L H, Hamdan M N, Al-Mazaydeh Z A, Bawadi R M, Rammaha M S, Zghoul A M, et al . Alu-repeat polymorphism in the tissue plasminogen activator (t-PA) gene, seminal t-PA concentration, and male fertility impairment: A case-control study. IJRM 2020; 18 (8) :571-578
URL: http://ijrm.ir/article-1-1510-en.html
URL: http://ijrm.ir/article-1-1510-en.html
Lubna Hamid Tahtamouni * 
1, Mahmoud Nael Hamdan2 , Zainab Ali Al-Mazaydeh2 , Randa Mahmoud Bawadi3 , Majdoleen Sobhi Rammaha2 , Ahmad Mohammad Zghoul2 , Mamoun Ahmad Ahram3 , Salem Yasin2
1, Mahmoud Nael Hamdan2 , Zainab Ali Al-Mazaydeh2 , Randa Mahmoud Bawadi3 , Majdoleen Sobhi Rammaha2 , Ahmad Mohammad Zghoul2 , Mamoun Ahmad Ahram3 , Salem Yasin2
1- Department of Biology and Biotechnology, Faculty of Science, the Hashemite University, Zarqa, Jordan. Department of Biochemistry and Molecular Biology, College of Natural Sciences, Colorado State University, Fort Collins, Colorado, USA. , lubnatahtamuni@hu.edu.jo
2- Department of Biology and Biotechnology, Faculty of Science, the Hashemite University, Zarqa, Jordan.
3- Department of Physiology and Biochemistry, School of Medicine, the University of Jordan, Amman, Jordan.
2- Department of Biology and Biotechnology, Faculty of Science, the Hashemite University, Zarqa, Jordan.
3- Department of Physiology and Biochemistry, School of Medicine, the University of Jordan, Amman, Jordan.
Abstract: (1943 Views)
Background: Tissue plasminogen activator (t-PA) is a protein involved in the fibrinolytic system that catalyzes the conversion of plasminogen into the active plasmin. The activity of t-PA is controlled by plasminogen activator inhibitor-1. t-PA has crucial functions during spermatogenesis. One polymorphism was reported for t-PA gene, either the presence of a 300-bp Alu-repeat (Alu+) or its absence (Alu-).
Objective: The current work aimed at studying the association between Alu polymorphism in the t-PA gene and male infertility.
Materials and Methods: Using polymerase chain reaction on genomic DNA isolated from the blood of 79 participants, a region polymorphic for Alu element insertion in t-PA gene was amplified. In addition, total t-PA concentration, plasminogen activator inhibitor-1 /t-PA complex concentration, and t-PA activity in seminal plasma were measured by enzyme-linked immunosorbent assay.
Results: The results indicate that the percentage of infertile participants (n = 50) who were homozygous for t-PA Alu insertion (Alu+/+), heterozygous Alu+/- or homozygous for t-PA Alu deletion (Alu-/-) did not change significantly (p = 0.43, 0.81, and 0.85, respectively) when compared with the control participants (n = 29). On the other hand, a significant decrease (p = 0.0001) of t-PA total concentration in seminal plasma was observed in the infertile group in comparison with the control group. However, the results indicate that there is no association between the t-PA Alu different genotypes and the total t-PA seminal concentration in the infertile group when compared to the control group (p = 0.63).
Conclusion: Data obtained from the current study does not support an association between t-PA Alu polymorphism and t-PA seminal concentration or male infertility.
Objective: The current work aimed at studying the association between Alu polymorphism in the t-PA gene and male infertility.
Materials and Methods: Using polymerase chain reaction on genomic DNA isolated from the blood of 79 participants, a region polymorphic for Alu element insertion in t-PA gene was amplified. In addition, total t-PA concentration, plasminogen activator inhibitor-1 /t-PA complex concentration, and t-PA activity in seminal plasma were measured by enzyme-linked immunosorbent assay.
Results: The results indicate that the percentage of infertile participants (n = 50) who were homozygous for t-PA Alu insertion (Alu+/+), heterozygous Alu+/- or homozygous for t-PA Alu deletion (Alu-/-) did not change significantly (p = 0.43, 0.81, and 0.85, respectively) when compared with the control participants (n = 29). On the other hand, a significant decrease (p = 0.0001) of t-PA total concentration in seminal plasma was observed in the infertile group in comparison with the control group. However, the results indicate that there is no association between the t-PA Alu different genotypes and the total t-PA seminal concentration in the infertile group when compared to the control group (p = 0.63).
Conclusion: Data obtained from the current study does not support an association between t-PA Alu polymorphism and t-PA seminal concentration or male infertility.
Type of Study: Original Article |
Subject:
Fertility & Infertility
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