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Volume 19, Issue 6 (June 2021)
Int J Reprod BioMed. 2021, 19(6): 515-524 |
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Khazaei F, Ghanbari E, Khazaei M. Improved hormonal and oxidative changes by Royal Jelly in the rat model of PCOS: An experimental study. Int J Reprod BioMed. 2021; 19 (6) :515-524
URL: http://ijrm.ir/article-1-1793-en.html
URL: http://ijrm.ir/article-1-1793-en.html
1- Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran., Fertility and Infertility Research Center, Health Technology Institute, University Ave., Shahid Shiroudi Blvd., Kermanshah, Iran. Postal code: 6714869914
2- Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran., Fertility and Infertility Research Center, Health Technology Institute, University Ave., Shahid Shiroudi Blvd., Kermanshah, Iran. Postal code: 6714869914
3- Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran. ,mkhazaei1345@yahoo.com
2- Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran., Fertility and Infertility Research Center, Health Technology Institute, University Ave., Shahid Shiroudi Blvd., Kermanshah, Iran. Postal code: 6714869914
3- Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran. ,
Abstract: (4167 Views)
Background: Polycystic ovarian syndrome (PCOS) is an endocrine and complex metabolic disorder, associated with anovulation, changes in sex hormone, biochemical factors, and ovarian tissue. Royal jelly (RJ) has antioxidant and anti-inflammatory properties.
Objective: To examine the therapeutic effect of RJ on PCOS-related hormonal and biochemical changes in a rat model of PCOS.
Materials and Methods: In this experimental study, 42 female Wistar rats (weighing 180-200 gr, aged 10-12 week) were divided into six groups (n = 7/each): control; PCOS; RJ 100 mg/kg; RJ 200 mg/kg; PCOS + RJ 100 mg/kg; and PCOS + RJ 200 mg/kg. After 21 days, the animals were weighed and dissected. The serums were used for nitric oxide (NO) and ferric-reducing antioxidant power (FRAP) assay and estradiol and progesterone measurements. The ovaries were assessed for histological changes.
Results: PCOS increased estradiol and NO levels, and decreased progesterone and FRAP levels. In PCOS+ RJ groups, the progesterone (p =0.01) and FRAP levels (p ≤ 0.001) increased and the estradiol and NO (p ≤ 0.001) levels decreased significantly. Moreover, the number of mature follicles (p = 0.01) and corpus luteum increased (p ≤ 0.001), and ovarian and uterus weight deceased significantly (p ≤ 0.001).
Conclusion: RJ improved estradiol, progesterone, FRAP, and NO levels, and ovarian structure in the rat model of PCOS.
Objective: To examine the therapeutic effect of RJ on PCOS-related hormonal and biochemical changes in a rat model of PCOS.
Materials and Methods: In this experimental study, 42 female Wistar rats (weighing 180-200 gr, aged 10-12 week) were divided into six groups (n = 7/each): control; PCOS; RJ 100 mg/kg; RJ 200 mg/kg; PCOS + RJ 100 mg/kg; and PCOS + RJ 200 mg/kg. After 21 days, the animals were weighed and dissected. The serums were used for nitric oxide (NO) and ferric-reducing antioxidant power (FRAP) assay and estradiol and progesterone measurements. The ovaries were assessed for histological changes.
Results: PCOS increased estradiol and NO levels, and decreased progesterone and FRAP levels. In PCOS+ RJ groups, the progesterone (p =0.01) and FRAP levels (p ≤ 0.001) increased and the estradiol and NO (p ≤ 0.001) levels decreased significantly. Moreover, the number of mature follicles (p = 0.01) and corpus luteum increased (p ≤ 0.001), and ovarian and uterus weight deceased significantly (p ≤ 0.001).
Conclusion: RJ improved estradiol, progesterone, FRAP, and NO levels, and ovarian structure in the rat model of PCOS.
Type of Study: Original Article |
Subject:
Fertility & Infertility
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