International Journal of
Reproductive Biomedicine
Sat, Nov 23, 2024
[Archive]
Volume 19, Issue 8 (August 2021)
IJRM 2021, 19(8): 715-724 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Behdarvand-Margha Z, Ahangarpour A, Shahraki M, Komeili G, Khorsandi L. The effects of gallic acid and metformin on male reproductive dysfunction in diabetic mice induced by methylglyoxal: An experimental study. IJRM 2021; 19 (8) :715-724
URL: http://ijrm.ir/article-1-1921-en.html
URL: http://ijrm.ir/article-1-1921-en.html
Zeinab Behdarvand-Margha1 
, Akram Ahangarpour * 2, Mohammadreza Shahraki1 , Gholamreza Komeili1 , Layasadat Khorsandi3
, Akram Ahangarpour * 2, Mohammadreza Shahraki1 , Gholamreza Komeili1 , Layasadat Khorsandi3
1- Department of Physiology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
2- Department of Physiology, Faculty of Medicine, Physiology Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. ,akramahangarpour@gmail.com
3- Department of Anatomical Sciences, Faculty of Medicine, Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
2- Department of Physiology, Faculty of Medicine, Physiology Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. ,
3- Department of Anatomical Sciences, Faculty of Medicine, Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Abstract: (1651 Views)
Background: Diabetes mellitus is a disease that has reached a dangerous point. Today, nearly 500 million men and women around the world live with diabetes. Gallic acid (Gal) affects diabetes.
Objective: To evaluate the effects of Gal and metformin (met) on the levels of glucose, insulin, testosterone, luteinizing hormone (LH), follicle-stimulating hormone (FSH), sperm count, antioxidant status, and histological changes in the testes of diabetic mice induced by methylglyoxal (MGO).
Materials and Methods: In this experimental study, 50 male adult NMRI mice, weighting 25-30 gr, aged 3-4 months were randomly divided into five equal groups (n = 10/each). (i) Control (vehicle, normal saline), (ii) MGO (600 mg/kg/d) orally for 28 days, (iii) Gal (50 mg/kg/d), (iv) MGO+Gal, and (v) MGO+met (200 mg/kg/d). Gal and met were administered orally for 21 consecutive days after the induction of diabetes. Blood samples were taken at 24 hr after the latest doses of treatment. Histological assessment of the testis was done, and the epididymis sperm count was obtained. Antioxidant indices, glucose, insulin, LH, FSH, and testosterone levels were measured.
Results: In the MGO group compared to the control group, insulin, glucose (p = 0.001), LH (p = 0.04) and malondialdehyde (p = 0.001) were increased. However, the level of testosterone (p = 0.001), seminiferous tubule diameters, epithelial height, sperm count, superoxide dismutase activity (p = 0.02), and testis volume (p = 0.01) were decreased. The results indicated that Gal and met ameliorated the MGO effects.
Conclusion: These findings suggested that the animals receiving MGO became diabetic. According to the results, Gal and met can effectively prevent MGO-induced diabetes. The effect of Gal was equivalent and sometimes better than metformin.
Objective: To evaluate the effects of Gal and metformin (met) on the levels of glucose, insulin, testosterone, luteinizing hormone (LH), follicle-stimulating hormone (FSH), sperm count, antioxidant status, and histological changes in the testes of diabetic mice induced by methylglyoxal (MGO).
Materials and Methods: In this experimental study, 50 male adult NMRI mice, weighting 25-30 gr, aged 3-4 months were randomly divided into five equal groups (n = 10/each). (i) Control (vehicle, normal saline), (ii) MGO (600 mg/kg/d) orally for 28 days, (iii) Gal (50 mg/kg/d), (iv) MGO+Gal, and (v) MGO+met (200 mg/kg/d). Gal and met were administered orally for 21 consecutive days after the induction of diabetes. Blood samples were taken at 24 hr after the latest doses of treatment. Histological assessment of the testis was done, and the epididymis sperm count was obtained. Antioxidant indices, glucose, insulin, LH, FSH, and testosterone levels were measured.
Results: In the MGO group compared to the control group, insulin, glucose (p = 0.001), LH (p = 0.04) and malondialdehyde (p = 0.001) were increased. However, the level of testosterone (p = 0.001), seminiferous tubule diameters, epithelial height, sperm count, superoxide dismutase activity (p = 0.02), and testis volume (p = 0.01) were decreased. The results indicated that Gal and met ameliorated the MGO effects.
Conclusion: These findings suggested that the animals receiving MGO became diabetic. According to the results, Gal and met can effectively prevent MGO-induced diabetes. The effect of Gal was equivalent and sometimes better than metformin.
Type of Study: Original Article |
Subject:
Reproductive Endocrinology
References
1. Maresch CC, Stute DC, Alves MG, Oliveira PF, de Kretser DM, Linn Th. Diabetes-induced hyperglycemia impairs male reproductive function: A systematic review. Hum Reprod Update 2018; 24: 86-105. [DOI:10.1093/humupd/dmx033] [PMID]
2. Fabian UA, Charles-Davies MA, Fasanmade AA, Olaniyi JA, Oyewole OE, Owolabi MO, et al. Male sexual dysfunction, leptin, pituitary and gonadal hormones in Nigerian males with metabolic syndrome and type 2 diabetes mellitus. J Reprod Infertil 2016; 17: 17-25.
3. Oroojan AA, Ahangarpour A, Paknejad B, Zareian P, Hami Z, Abtahi SR. Effects of myricitrin and solid lipid nanoparticle-containing myricitrin on reproductive system disorders induced by diabetes in male mouse. World J Mens Health 2021; 39: 147-157. [DOI:10.5534/wjmh.190010] [PMID] [PMCID]
4. Maessen DEM, Stehouwer CDA, Schalkwijk CG. The role of methylglyoxal and the glyoxalase system in diabetes and other age-related diseases. Clin Sci (Lond) 2015; 128: 839-861. [DOI:10.1042/CS20140683] [PMID]
5. Dhar A, Dhar I, Jiang B, Desai KM, Wu L. Chronic methylglyoxal infusion by minipump causes pancreatic beta-cell dysfunction and induces type 2 diabetes in Sprague-Dawley rats. Diabetes 2011; 60: 899-908. [DOI:10.2337/db10-0627] [PMID] [PMCID]
6. Karimi J, Goodarzi MT, Tavilani H, Khodadadi I, Amiri I. Relationship between advanced glycation end products and increased lipid peroxidation in semen of diabetic men. Diabetes Res Clin Pract 2011; 91: 61-66. [DOI:10.1016/j.diabres.2010.09.024] [PMID]
7. Abdel-Moneim A, El-Twab SMA, Yousef AI, Reheim ESA, Ashour MB. Modulation of hyperglycemia and dyslipidemia in experimental type 2 diabetes by gallic acid and p-coumaric acid: The role of adipocytokines and PPARγ. Biomed Pharmacother 2018; 105: 1091-1097. [DOI:10.1016/j.biopha.2018.06.096] [PMID]
8. Punithavathi VR, Prince PSM, Kumar R, Selvakumari J. Antihyperglycaemic, antilipid peroxidative and antioxidant effects of gallic acid on streptozotocin induced diabetic Wistar rats. Eur J Pharmacol 2011; 650: 465-471. [DOI:10.1016/j.ejphar.2010.08.059] [PMID]
9. Oyagbemi AA, Omobowale TO, Saba AB, Adedara IA, Olowu ER, Akinrinde AS, et al. Gallic acid protects against cyclophosphamide-induced toxicity in testis and epididymis of rats. Andrologia 2016; 48: 393-401. [DOI:10.1111/and.12459] [PMID]
10. Lee BH, Hsu WH, Chang YY, Kuo HF, Hsu YW, Pan TM. Ankaflavin: A natural novel PPARγ agonist upregulates Nrf2 to attenuate methylglyoxal-induced diabetes in vivo. Free Radic Biol Med 2012; 53: 2008-2016. [DOI:10.1016/j.freeradbiomed.2012.09.025] [PMID]
11. Patel SS, Goyal RK. Cardioprotective effects of gallic acid in diabetes-induced myocardial dysfunction in rats. Pharmacognosy Res 2011; 3: 239-245. [DOI:10.4103/0974-8490.89743] [PMID] [PMCID]
12. Abdelsamia EM, Khaleel SA, Balah A, Abdel Baky NA. Curcumin augments the cardioprotective effect of metformin in an experimental model of type I diabetes mellitus; impact of Nrf2/HO-1 and JAK/STAT pathways. Biomed Pharmacother 2019; 109: 2136-2144. [DOI:10.1016/j.biopha.2018.11.064] [PMID]
13. Ahangarpour A, Oroojan AA, Radan M. Effect of aqueous and hydro-alcoholic extracts of lettuce (Lactuca sativa) seed on testosterone level and spermatogenesis in NMRI mice. Iran J Reprod Med 2014; 12: 65-72.
14. Amaral S, Oliveira PJ, Ramalho-Santos J. Diabetes and the impairment of reproductive function: Possible role of mitochondria and reactive oxygen species. Curr Diabetes Rev 2008; 4: 46-54. [DOI:10.2174/157339908783502398] [PMID]
15. Ahangarpour A, Oroojan AA, Heydari H. [Effect of hydro-alcoholic extract of dorema aucheri on serum levels of testosterone, FSH and sperm count in nicotinamide-STZ-induced diabetic rat models]. J Adv Med Biomed Res 2013; 21: 22-31. (in Persian)
16. Alves MG, Martins AD, Cavaco JE, Socorro S, Oliveira PF. Diabetes, insulin-mediated glucose metabolism and Sertoli/blood-testis barrier function. Tissue Barriers 2013; 1: e23992. [DOI:10.4161/tisb.23992] [PMID] [PMCID]
17. Cheng ASh, Cheng YH, Lee ChY, Chung ChY, Chang WCh. Resveratrol protects against methylglyoxal-induced hyperglycemia and pancreatic damage in vivo. Nutrients 2015; 7: 2850-2865. [DOI:10.3390/nu7042850] [PMID] [PMCID]
18. Kender Z, Fleming T, Kopf S, Torzsa P, Grolmusz V, Herzig S, et al. Effect of metformin on methylglyoxal metabolism in patients with type 2 diabetes. Exp Clin Endocrinol Diabetes 2014; 122: 316-319. [DOI:10.1055/s-0034-1371818] [PMID]
19. Engelen L, Stehouwer CDA, Schalkwijk CG. Current therapeutic interventions in the glycation pathway: Evidence from clinical studies. Diabetes Obes Metab 2013; 15: 677-689. [DOI:10.1111/dom.12058] [PMID]
20. Latha RC, Daisy P. Insulin-secretagogue, antihyperlipidemic and other protective effects of gallic acid isolated from Terminalia bellerica Roxb. In streptozotocin-induced diabetic rats. Chem Biol Interact 2011; 189: 112-118. [DOI:10.1016/j.cbi.2010.11.005] [PMID]
21. Vinayagam R, Jayachandran M, Xu B. Antidiabetic effects of simple phenolic acids: A comprehensive review. Phytother Res 2016; 30: 184-199. [DOI:10.1002/ptr.5528] [PMID]
22. Variya BC, Bakrania AK, Patel SS. Antidiabetic potential of gallic acid from Emblica officinalis: Improved glucose transporters and insulin sensitivity through PPAR-γ and Akt signaling. Phytomedicine 2020; 73: 152906. [DOI:10.1016/j.phymed.2019.152906] [PMID]
23. Yousuf MJ, Vellaichamy E. Protective activity of gallic acid against glyoxal-induced renal fibrosis in experimental rats. Toxicol Rep 2015; 2: 1246-1254. [DOI:10.1016/j.toxrep.2015.07.007] [PMID] [PMCID]
24. Gandhi GR, Jothi G, Antony PJ, Balakrishna K, Paulraj MG, Ignacimuthu S, et al. Gallic acid attenuates high-fat diet fed-streptozotocin-induced insulin resistance via partial agonism of PPARgamma in experimental type 2 diabetic rats and enhances glucose uptake through translocation and activation of GLUT4 in PI3K/p-Akt signaling pathway. Eur J Pharmacol 2014; 745: 201-216. [DOI:10.1016/j.ejphar.2014.10.044] [PMID]
25. Yigitturk G, Acara AC, Erbas O, Oltulu F, Yavasoglu NUK, Uysal A, et al. The antioxidant role of agomelatine and gallic acid on oxidative stress in STZ induced type I diabetic rat testes. Biomed Pharmacother 2017; 87: 240-246. [DOI:10.1016/j.biopha.2016.12.102] [PMID]
26. Condorelli R, Calogero AE, La Vignera S. Relationship between testicular volume and conventional or nonconventional sperm parameters. Int J Endocrinol 2013; 2013: 145792. [DOI:10.1155/2013/145792] [PMID] [PMCID]
27. Johnson L. Evaluation of the human testis and its age-related dysfunction. Prog Clin Biol Res 1989; 302: 35-60.
28. Kim TB, Park IN. Larger testicular volume is independently associated with favorable indices of lung function. Tuberc Respir Dis 2017; 80: 385-391. [DOI:10.4046/trd.2016.0027] [PMID] [PMCID]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
