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Volume 12, Issue 5 (6-2014)
IJRM 2014, 12(5): 307-0 |
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Rahimipour M, Talebi A R, Anvari M, Abbasi Sarcheshmeh A, Omidi M. Saccharin consumption increases sperm DNA fragmentation and apoptosis in mice. IJRM 2014; 12 (5) :307-0
URL: http://ijrm.ir/article-1-543-en.html
URL: http://ijrm.ir/article-1-543-en.html
Marzieh Rahimipour1 
, Ali Reza Talebi * 2, Morteza Anvari1 , Abolghasem Abbasi Sarcheshmeh1 , Marjan Omidi1
, Ali Reza Talebi * 2, Morteza Anvari1 , Abolghasem Abbasi Sarcheshmeh1 , Marjan Omidi1
1- Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2- Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran ,prof_talebi@ssu.ac.ir
2- Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran ,
Abstract: (2777 Views)
Background: Saccharin is an artificial non-caloric sweetener that used to sweeten products such as drinks, candies, medicines, and toothpaste, but our bodies cannot metabolize it. Sodium saccharin is considered as an important factor in tumor promotion in male rats but not in humans.
Objective: The objective of this study was to investigate the effect of saccharin consumption on sperm parameters and apoptosis in adult mice.
Materials and Methods: Totally 14 adult male mice were divided into 2 groups. Group 1 served as control fed on basal diet and group 2 or experimental animals received distilled water containing saccharin (0.2% w/v) for 35 days. After that, the left cauda epididymis of each mouse was cut and placed in Ham’s F10. Swimmed-out spermatozoa were used to analyze count, motility, morphology (Pap-staining) and viability (eosin-Y staining). Sperm DNA integrity, as an indicator of apoptosis, was assessed by SCD (sperm chromatin dispersion) and terminal deoxynucleotidyl transferase (TUNEL) assay.
Results: Following saccharin consumption, we had a reduction in sperm motility with respect to control animals (p=0.000). In addition, the sperm count diminished (17.70±1.11 in controls vs. 12.80±2.79 in case group, p=0.003) and the rate of sperm normal morphology decreased from 77.00±6.40 in control animals into 63.85±6.81 in saccharin-treated mice (p=0.001). Also, we saw a statistically significant increase in rates of sperm DNA damage and apoptosis in experimental group when compared to control one (p=0.001, p=0.002 respectively).
Conclusion: Saccharin consumption may have negative effects on sperm parameters, and increases the rate of sperm DNA fragmentation and apoptosis in mice.
Objective: The objective of this study was to investigate the effect of saccharin consumption on sperm parameters and apoptosis in adult mice.
Materials and Methods: Totally 14 adult male mice were divided into 2 groups. Group 1 served as control fed on basal diet and group 2 or experimental animals received distilled water containing saccharin (0.2% w/v) for 35 days. After that, the left cauda epididymis of each mouse was cut and placed in Ham’s F10. Swimmed-out spermatozoa were used to analyze count, motility, morphology (Pap-staining) and viability (eosin-Y staining). Sperm DNA integrity, as an indicator of apoptosis, was assessed by SCD (sperm chromatin dispersion) and terminal deoxynucleotidyl transferase (TUNEL) assay.
Results: Following saccharin consumption, we had a reduction in sperm motility with respect to control animals (p=0.000). In addition, the sperm count diminished (17.70±1.11 in controls vs. 12.80±2.79 in case group, p=0.003) and the rate of sperm normal morphology decreased from 77.00±6.40 in control animals into 63.85±6.81 in saccharin-treated mice (p=0.001). Also, we saw a statistically significant increase in rates of sperm DNA damage and apoptosis in experimental group when compared to control one (p=0.001, p=0.002 respectively).
Conclusion: Saccharin consumption may have negative effects on sperm parameters, and increases the rate of sperm DNA fragmentation and apoptosis in mice.
Type of Study: Original Article |
References
1. Mitchell H. Sweeteners and sugar alternatives in food technology. 1th Ed. Blackwell; 2006. [DOI:10.1002/9780470996003]
2. Schardt D. Sweet nothings. Nutr Act 2004; 9: 8-11.
3. No DCR. Saccharin and its salts. 2th Ed. 1987.
4. Zurlo J, Squire RA. Is saccharin safe? Animal testing revisited. J Natl Cancer Inst 1998; 90: 2-3. [DOI:10.1093/jnci/90.1.2]
5. Turner SD, Tinwell H, Piegorsch W, Schmezer P, Ashby J. The male rat carcinogens limonene and sodium saccharin are not mutagenic to male Big BlueTM rats. Mutagenesis 2001; 16: 329-332. [DOI:10.1093/mutage/16.4.329]
6. Wolff S, Rodin B. Saccharin-induced sister chromatid exchanges in Chinese hamster and human cells. Science 1978; 200: 543-545. [DOI:10.1126/science.644315]
7. Hikim AS, Swerdloff RS. Hormonal and genetic control of germ cell apoptosis in the testis. Rev Reprod 1999; 4: 38-47. [DOI:10.1530/ror.0.0040038]
8. Porcelli F, Meggiolaro D, Carnevali A, Ferrandi B. Fas ligand in bull ejaculated spermatozoa: a quantitative immunocytochemical study. Acta Histochem 2006; 108: 287-292. [DOI:10.1016/j.acthis.2006.05.006]
9. Nagata S. Fas ligand-induced apoptosis. Annu Rev Genet 1999; 33: 29-55. [DOI:10.1146/annurev.genet.33.1.29]
10. Ozmen B, Koutlaki N, Youssry M, Diedrich K, Al-Hasani S. DNA damage of human spermatozoa in assisted reproduction: origins, diagnosis, impacts and safety. Reprod Biomed Online 2007; 14: 384-395. [DOI:10.1016/S1472-6483(10)60883-8]
11. Liu W, Staecker H, Stupak H, Malgrange B, Lefebvre P, Van De Water TR. Caspase inhibitors prevent cisplatin-induced apoptosis of auditory sensory cells. Neuroreport 1998; 9: 2609-2614. [DOI:10.1097/00001756-199808030-00034]
12. Wyllie AH, Kerr JF, Currie AR. Cell death: the significance of apoptosis. Int Rev Cytol 1980; 68: 251. [DOI:10.1016/S0074-7696(08)62312-8]
13. Talebi AR. Sperm Nuclear Maturation: a basic and clinical approach. 1th Ed. New York, Nova Science Publishers; 2011.
14. Schulte RT, Ohl DA, Sigman M, Smith GD. Sperm DNA damage in male infertility: etiologies, assays, and outcomes. J Assist Reprod Genet 2010; 27: 3-12. [DOI:10.1007/s10815-009-9359-x]
15. Zini A, Bielecki R, Phang D, Zenzes MT. Correlations between two markers of sperm DNA integrity, DNA denaturation and DNA fragmentation, in fertile and infertile men. Fertil Steril 2001; 75: 674. [DOI:10.1016/S0015-0282(00)01796-9]
16. Makler A. The improved ten-micrometer chamber for rapid sperm count and motility evaluation. Fertil Steril 1980; 33: 337. [DOI:10.1016/S0015-0282(16)44606-6]
17. Kermani-Alghoraishi M, Anvari M, Talebi AR, Amini-Rad O, Ghahramani R, Miresmaili SM. The effects of acrylamide on sperm parameters and membrane integrity of epididymal spermatozoa in mice. Eur J Obstet Gynecol Reprod Biol 2010; 153: 52-55. [DOI:10.1016/j.ejogrb.2010.07.008]
18. Kruger TF, Menkveld R, Stander F, Lombard C, Van der Merwe J, Van Zyl J, et al. Sperm morphologic features as a prognostic factor in in vitro fertilization. Fertil Steril 1986; 46: 1118. [DOI:10.1016/S0015-0282(16)49891-2]
19. Fernández JL, Muriel L, Rivero MT, Goyanes V, Vazquez R, Alvarez JG. The sperm chromatin dispersion test: a simple method for the determination of sperm DNA fragmentation. J Androl 2003; 24: 59-66.
20. Gandini L, Lombardo F, Paoli D, Caponecchia L, Familiari G, Verlengia C, et al. Study of apoptotic DNA fragmentation in human spermatozoa. Hum Reprod 2000; 15: 830-839. [DOI:10.1093/humrep/15.4.830]
21. Ellwein LB, Cohen SM. The health risks of saccharin revisited. Crit Rev Toxicol 1990; 20: 311-326. [DOI:10.3109/10408449009089867]
22. Dybing E. Development and implementation of the IPCS conceptual framework for evaluating mode of action of chemical carcinogens. Toxicology 2002; 181: 121-125. [DOI:10.1016/S0300-483X(02)00266-4]
23. Sarıözkan S, Bucak MN, Canturk F, Özdamar S, Yay A, Tuncer PB, et al. The effects of different sugars on motility, morphology and DNA damage during the liquid storage of rat epididymal sperm at 4°C. Cryobiology 2012; 65: 93-97. [DOI:10.1016/j.cryobiol.2012.05.007]
24. Corcuera B, Marigorta P, Sagüés A, Saiz-Cidoncha F, Pérez-Gutiérrez J. Effect of lactose and glycerol on the motility, normal apical ridge, chromatin condensation and chromatin stability of frozen boar spermatozoa. Theriogenology 2007; 67: 1150-1157. [DOI:10.1016/j.theriogenology.2007.01.002]
25. Sánchez R, Risopatrón J, Schulz M, Villegas J, Isachenko V, Kreinberg R, et al. Canine sperm vitrification with sucrose: effect on sperm function. Andrologia 2011; 43: 233-241. [DOI:10.1111/j.1439-0272.2010.01054.x]
26. Dasgupta J, Elliott RA, Doshani A, Tincello DG. Enhancement of rat bladder contraction by artificial sweeteners via increased extracellular Ca< sup> 2+ influx. Toxicol Appl Pharmacol 2006; 217: 216-224. [DOI:10.1016/j.taap.2006.09.004]
27. Andreatta M, Mu-oz S, Lantieri M, Eynard A, Navarro A. Artificial sweetener consumption and urinary tract tumors in Cordoba, Argentina. Prev Med 2008; 47: 136-139. [DOI:10.1016/j.ypmed.2008.03.015]
28. Tavalaee M, Nasr-Esfahani MH, Deemeh MR. Etiology and evaluation of sperm chromatin anomalies. Int J Fertil Steril 2008; 2: 1-8.
29. Kensler TW, Trush MA. Role of oxygen radicals in tumor promotion. Environ Mutagen 1984; 6: 593-616. [DOI:10.1002/em.2860060412]
30. Al-Saleh AM. Effect of Artificial Sweeteners on Insulin Secretion, ROS, and Oxygen Consumption in Pancreatic [Beta]-cells. Boston University, Boston, 2010.
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