Volume 12, Issue 8 (8-2014)                   IJRM 2014, 12(8): 573-0 | Back to browse issues page

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Dehghan T, Mozdarani H, Khoradmehr A, Kalantar S M, Bakhshandeh M, Bouzarjomehri F, et al . Evaluation of embryo quality after concurrent use of ovarian stimulating hormones and gamma irradiation. IJRM 2014; 12 (8) :573-0
URL: http://ijrm.ir/article-1-569-en.html
Evaluation of embryo quality after concurrent use of ovarian stimulating hormones and gamma irradiation
Tahere Dehghan1 , Hossein Mozdarani * 2, Arezoo Khoradmehr3 , Seyed Mehdi Kalantar3 , Mohsen Bakhshandeh4 , Fathollah Bouzarjomehri5 , Seyed Milad Kalantar6 , Morteza Sepehr Javan7
1- Shahid Beheshti University of Medical Sciences, Tehran, Iran
2- Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran , mozdarah@modares.ac.ir
3- Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
4- Department of Radiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5- Department of Medical Physics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
6- Department of Biotechnology, School of Agriculture, Islamic Azad University, Isfahan, Iran
7- Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Abstract:   (2393 Views)
Background: Radiotherapy has many side effects on fertilization in young women. Radiation can lead to ovarian failure in women who underwent abdomen or pelvic radiotherapy.
Objective: This study helps us to investigate ovarian response of NMRI female mice to ovarian stimulating hormones (PMSG, HCG) after whole-body gamma irradiation.
Materials and Methods: 45 pregnant mice were divided into two groups of control and experimental. The experimental group was classified into three sub-groups: Irradiation group (2 or 4Gy),Superovulation group (10 or 15IU),and superovulation and gamma-radiation group (2Gy & 10IU, 2Gy & 15IU, 4Gy & 10IU,4Gy & 15IU). Female mice were killed and embryos were removed from oviduct .The number of embryos cells counted and the quality of them was evaluated in each group. Kruskal-Wallis test and Mann-Whitney test were used to analyze the data.
Results: There was a significant difference in the number of 2-4 cells grade D embryos in 2Gy & 15IU group compared with control and 2Gy groups (p=0.01), and the number of embryos in 4Gy group was more than in 10IU and 15IU (p=0.03) and 2Gy & 15IU groups (p=0.01). It was more significantly embryos in 4Gy & 15IU group compared to 2Gy & 15IU group (p=0.01).In addition There were no significant differences in the number of 2-4 cells grades A, B and C embryos and also number of 4-8 cells grades A, B and C, D embryos in groups.
Conclusion: The concurrent use of ovulation stimulating hormones and gamma rays ameliorates this problem of drastic decrease in number of living embryos due to whole-body irradiation.
Keywords: PMSG-HCG, Gamma rays, Whole body irradiation
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Type of Study: Original Article |
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