Volume 13, Issue 4 (5-2015)                   IJRM 2015, 13(4): 237-242 | Back to browse issues page

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Faramarzi A, Khalili M A, Soleimani M. First successful pregnancies following embryo selection using Time-lapse technology in Iran: Case report. IJRM 2015; 13 (4) :237-242
URL: http://ijrm.ir/article-1-639-en.html
First successful pregnancies following embryo selection using Time-lapse technology in Iran: Case report
Azita Faramarzi1 , Mohammad Ali Khalili * 2, Mehrdad Soleimani1
1- Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2- Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , khalili59@hotmail.com
Abstract:   (4128 Views)
Background: Embryo selection is a vital part of in vitro fertilization (IVF) programs, with morphology-based grading systems having been widely used for decades. Time-lapse imaging combined with embryo morph kinetics may proffer a non-invasive means for improving embryo selection. We report the first ongoing and chemical pregnancies using Time-lapse embryo scope to select best embryos for transfer in Iran.
Cases: A case with tubal factor infertility was admitted to IVF program with normozoospermia. After ovarian hyper stimulation, 6 COCs were retrieved and inseminated with 25,000 progressive sperms/ oocyte. Five zygotes were placed individually into the micro wells of equilibrated embryo scope dish for Time-lapse observation, and incubated at 37°C, 5% CO2. On day 3, single embryo transfer (SET) took place based on kinetic parameters of the embryos. Clinical pregnancy was confirmed 7 weeks after SET. The second case with history of previous ICSI failure was admitted with azoospermia. Nine MII oocytes underwent ICSI, and incubated in Time-lapse facilities. The rest of procedures were followed as described for case 1. Chemical pregnancy was confirmed 15 days after SET.
Conclusion: This approach opens a way to select best embryo non-invasively for SET; thus, increasing implantation, while reducing multiple pregnancy complications.
Keywords: Kinetics, Time-lapse, Embryo, Selection.
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Type of Study: Original Article |
References
1. Khalili M, Moinia F. Role of embryo morphology and cumulative embryo score in pregnancy outcome from in vitro fertilization and intracytoplasmic sperm injection cycles. Mid East Fertil Soc J 2002; 7: 231-236.
2. Kirkegaard K, Agerholm IE, Ingerslev HJ. Time-lapse monitoring as a tool for clinical embryo assessment. Hum Reprod 2012; 27: 1277–1285. [DOI:10.1093/humrep/des079]
3. Desai N, Ploskonka S, Goodman LR, Austin C, Goldberg J, Falcone T. Analysis of embryo morphokinetics, multinucleation and cleavage anomalies using continuous time-lapse monitoring in blastocyst transfer cycles. Reprod Biol Endocrinol 2014, 12: 54. [DOI:10.1186/1477-7827-12-54]
4. Chamayou S, Patrizio P, Storaci G, Tomaselli V, Alecci C, Ragolia C, et al. The use of morphokinetic parameters to select all embryos with full capacity to implant. J Assist Reprod Genet 2013; 30: 703–710. [DOI:10.1007/s10815-013-9992-2]
5. Meseguer M, Herrero J, Tejara A, Hilligoe KM, Ramsing Nb, Remohi J. The use of morphokinetic as a predictor of embryo implantation. Human Reprod 2011; 26: 2658-2671. [DOI:10.1093/humrep/der256]
6. HerreroJ, Meseguer M. Selection of high potential embryos using time-lapse imaging: the era of morphokinetics. Fertil Steril 2013; 99: 1030–1034. [DOI:10.1016/j.fertnstert.2013.01.089]
7. Seli E, Robert C, Sirard MA. OMICS in assisted reproduction: possibilities and pitfalls. Mol Hum Reprod 2010; 16: 513–530. [DOI:10.1093/molehr/gaq041]
8. Montag M, Liebenthron J, Koster M. Which morphological scoring system is relevant in human embryo development? Placenta 2011; 32:S252–S256. [DOI:10.1016/j.placenta.2011.07.009]
9. Chen AA, Tan L, Suraj V, Reijo Pera R, Shen S. Biomarkers identified with time-lapse imaging: discovery, validation, and practical application. Fertil Steril 2013; 99: 1035–1043. [DOI:10.1016/j.fertnstert.2013.01.143]
10. Conaghan J, Chen AA, Willman SP, Ivani K, Chenette PE, Boostanfar R, et al. Improving embryo selection using a computer-automated time-lapse image analysis test plus day 3 morphology: results from a prospective multicenter trial. Fertil Steril 2013; 100: 412–419. [DOI:10.1016/j.fertnstert.2013.04.021]
11. Nygren KG, Sullivan E, Zegers-Hochschild F, Mansour R, Ishihara O, Adamson GD, et al. International Committee for Monitoring Assisted Reproductive Technology (ICMART) world report: assisted reproductive technology 2003. Fertil Steril 2011; 95: 2209–2222. [DOI:10.1016/j.fertnstert.2011.03.058]
12. Meseguer M, Rubio I, Cruz M, Basile N, Marcos J, Requena A. Embryo incubation and selection in a time-lapse monitoring system improves pregnancy outcome compared with a standard incubator: a retrospective cohort study. Fertil Steril 2012; 98:1481–1489. [DOI:10.1016/j.fertnstert.2012.08.016]
13. Park H, Bergh C, Selleskog U, Thurin-Kjellberg A, Lundin K. No benefit of culturing embryos in a closed system compared with a conventional incubator in terms of number of good quality embryos: results from an RCT. Hum Reprod 2015; 30: 268-275. [DOI:10.1093/humrep/deu316]
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