Volume 14, Issue 7 (7-2016)                   IJRM 2016, 14(7): 459-464 | Back to browse issues page

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Alizadeh N, Mosaferi E, Farzadi L, Majidi J, Monfaredan A, Yousefi B et al . Frequency of null allele of Human Leukocyte Antigen-G (HLA-G) locus in subjects to recurrent miscarriage. IJRM 2016; 14 (7) :459-464
URL: http://ijrm.ir/article-1-766-en.html
Frequency of null allele of Human Leukocyte Antigen-G (HLA-G) locus in subjects to recurrent miscarriage
Nazila Alizadeh1 , Elnaz Mosaferi1 , Laya Farzadi2 , Jafar Majidi1 , Amir Monfaredan3 , Bahman Yousefi4 , Behzad Baradaran * 5
1- Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2- Women Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
3- Department of Hematology, Faculty of Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
4- Students Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
5- Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran , behzad_im@yahoo.com
Abstract:   (3339 Views)
Background: Human leukocyte antigen-G (HLA-G) is a non-classical class I molecule highly expressed by extravillous cytotrophoblast cells. Due to a single base pair deletion, its function can be compensated by other isoforms. Investigating the frequency of null allele in Recurrent Miscarriage (RM) subjects could be useful in understanding the relationship between frequency of this allele and RM in a given population. 
Objective: This study aimed to determine the frequency of HLA-G*0105N null allele and its potential association with down-regulation of HLA-G in subjects with RM. 
Materials and Methods: Western blotting was used to assess the level of HLA-G protein expression. For investigating the frequency of HLA-G*0105N null allele in RM subjects, PCR-RFLP method was used. Exon 3 of HLA-G gene was amplified by polymerase chain reaction (PCR). Subsequently, PpuM-1 enzyme was employed to digest the PCR products and fragments were analyzed using gel electrophoresis. 
Results: Digestion using restriction enzyme showed the presence of heterozygous HLA-G*0105N null allele in 10% of the test population. Western blotting results confirmed the decrease in expression of HLA-G in the placental tissue of subjects with RM compared to subjects who could give normal birth. 
Conclusion: The frequency of heterozygous HLA-G*0105N null allele was high to some extent in subjects with RM. The mutation rate in subjects suggested that there is a significant association between RM and frequency of mutations in this allele.
Keywords: Allele, HLA-G*0105N, Recurrent miscarriage, RFLPs
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Type of Study: Original Article |
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