Volume 14, Issue 9 (9-2016)                   IJRM 2016, 14(9): 567-576 | Back to browse issues page

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Esmaeli A, Moshrefi M, Shamsara A, Eftekhar-vaghefi S H, Nematollahi-mahani S N. Xeno-free culture condition for human bone marrow and umbilical cord matrix-derived mesenchymal stem/stromal cells using human umbilical cord blood serum. IJRM 2016; 14 (9) :567-576
URL: http://ijrm.ir/article-1-785-en.html
Xeno-free culture condition for human bone marrow and umbilical cord matrix-derived mesenchymal stem/stromal cells using human umbilical cord blood serum
Azadeh Esmaeli1 , Mojgan Moshrefi1 , Ali Shamsara2 , Seyed Hasan Eftekhar-vaghefi3 , Seyed Noureddin Nematollahi-mahani * 4
1- Physiology Research Center, Institute of neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
2- Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
3- Department of Anatomy, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
4- Physiology Research Center, Institute of neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran , nnematollahi@kmu..ac.ir
Abstract:   (4088 Views)
Background: Fetal bovine serum (FBS) is widely used in cell culture laboratories,risk of zoonotic infections and allergic side effects create obstacles for its use inclinical trials. Therefore, an alternative supplement with proper inherent growthpromotingactivities is demanded.
Objective: To find FBS substitute, we tested human umbilical cord blood serum(hUCS) for proliferation of human umbilical cord matrix derived mesenchymal stemcells (hUC-MSCs) and human bone marrow-derived mesenchymal cells (hBMMSCs).
Materials and Methods: Umbilical cord blood of healthy neonates, delivered byCaesarian section, was collected and the serum was separated. hUC-MSCs andhBM-MSCs were isolated and characterized by assessment of cell surface antigensby flow cytometry, alkaline phosphatase activity and osteogenic/adipogenicdifferentiation potential. The cells were then cultured in Iscove's ModifiedDulbecco's Medium (IMDM) by conventional methods in three preparations: 1- withhUCS, 2- with FBS, and 3- without serum supplements. Cell proliferation wasmeasured using WST-1 assay, and cell viability was assessed by trypan bluestaining.
Results: The cells cultured in hUCS and FBS exhibited similar morphology andmesenchymal stem cells properties. WST-1 proliferation assay data showed nosignificant difference between the proliferation rate of either cells following hUCSand FBS supplementation. Trypan blue exclusion dye test also revealed nosignificant difference for viability between hUCS and FBS groups. A significantdifference was detected between the proliferation rate of stem cells cultured inserum-supplemented medium compared with serum-free medium.
Conclusion: Our results indicate that human umbilical cord serum can effectivelysupport proliferation of hBM-MSCS and hUC-MSCs in vitro and can be used as anappropriate substitute for FBS, especially in clinical studies.
Keywords: Bone marrow, Cell proliferation, Cord blood, Mesenchymal stem/stromal cells, Umbilical cord, Wharton jelly
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Type of Study: Original Article |
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