Volume 5, Issue 5 (7-2007)                   IJRM 2007, 5(5): 177-181 | Back to browse issues page

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Mostajeran F, Naderi M, Adibi S. Effects of melatonin on histopathological changes after experimental ovarian torsion-detorsion in cat. IJRM 2007; 5 (5) :177-181
URL: http://ijrm.ir/article-1-88-en.html
Effects of melatonin on histopathological changes after experimental ovarian torsion-detorsion in cat
Fatemeh Mostajeran * 1, Maryam Naderi2 , Shahriar Adibi3
1- Department of Obstetric and Gynecology, Isfahan University of Medical Sciences, Isfahan, Iran , mostajeran@med.mui.ac.ir
2- Department of Obstetric and Gynecology, Isfahan University of Medical Sciences, Isfahan, Iran
3- Torabienejad Dental Research Center, Isfahan University
Abstract:   (1977 Views)
Background: During the detorsion of a torsioned ovary, oxidant agents are released and melatonin as an antioxidant can reduce ischemia. We studied the histopathological changes after using melatonin on experimental torsioned ovary in cat.
Objective: The aim of this experimental study was to investigate the effects of Melatonin on histopathological changes in torsion – detorsion injury in cat ovary.
Materials and Methods: An adnexal torsion – detorsion model was created by using 20 adult cats randomly divided equally in to 2 groups of Saline and Melatonin. Ischemia was induced by iathrogenic 360° clockwise torsion of the cat adnex for 3 hr. Reperfusion was achieved for 3 hr. Melatonin or saline were injected intra peritoneally (10mg/kg) 30 min before ovarian detorsion in both groups. After 3 hr of ovarian detorsion, ovarian tissue was removed and fixed in 10% formalin solution, embedded in paraffin and evaluated for ischemic indices.
Results: Histological examination showed a significant improvement in ovarian morphology in the melatonin treated cats. Edema and vasoconstriction in saline group were more severe than Melatonin group (p-value = 0.009). Hemorrhage and leukocyte infiltration were also more obvious in saline group (p-value 0.0018)
Conclusion: Our results demonstrated that Melatonin administration reduced ovarian histopathological damage due to oxidative injury associated with torsion.
Keywords: Melatonin, Saline, Ischemia, Reperfusion, Cat.
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Type of Study: Original Article |
References
1. Chen M, Chen CD, Yang YS. Torsion of the previously normal uterine adnexa. Evaluation of the correlation between the pathological changes and the clinical characteristics. Acta obstet Gynecol scand 2001; 85:58-61. [DOI:10.1034/j.1600-0412.2001.800111.x]
2. Haskins T, Shull B. Adnexal torsion: a mind - twisting diagnosis. South Med J 1986; 79: 576- 577. [DOI:10.1097/00007611-198605000-00013]
3. Meyer JS, Harman CM, Harty MP , Markowitz RI, Hubba AM, Bellah RD. Ovarian torsion: Clinical and imaging presentation in children. J Pediatr Surg 1995; 30:1433 -1436. [DOI:10.1016/0022-3468(95)90399-2]
4. Taskin O, Birincioglu M, Aydin A, Buhur A, Burak F, Yilmaz I, et al. The effects of twisted ischemic adnexa managed by detorsion on Ovarion viability and histology: an ischemia-reperfusion rodent model. Hum Reprod 1998; 13: 2823-2827. [DOI:10.1093/humrep/13.10.2823]
5. Kaakaji Y, Nghiem HV, Nodell C, Winter TC. Sonography of obstetric and Gynecologic emergencies: part II, gynecologic emergencies . AM J Roentgend 2000; 174:651-656. [DOI:10.2214/ajr.174.3.1740651]
6. Bird JE, Milhoan K, Wilson CB, Young SG, Mundy CA. Ischemic acute renal failure and antioxidant therapy in the rat: the relation between glomerular and tubular dysfurction. J Din Invest 1988; 81:1630-1638. [DOI:10.1172/JCI113498]
7. Baker GL, Corry RJ, Autor AP. Oxygen free radical induced damage in kidneys subjected to warm ischemia and reperfusion: protection effect of superoxide dismutase. Ann Surg 1985; 202:628-641. [DOI:10.1097/00000658-198511000-00016]
8. Gilham B, Papa Chri Stodoulou Dk, Thomas Jh. Will's Biochemical Basis of Medicine 3rd Ed. Butterworth - Heinemann, London 1997: 343-354.
9. Breen AP, Murphy JA. Reactions of oxyl radicals with DNA. Free Radic Biol Med 1995 ; 18: 1033-1077. [DOI:10.1016/0891-5849(94)00209-3]
10. Ames BN. Endogenous dna damage as related to cancer and aging. Mutat Res 1989 ; 214: 41-46 [DOI:10.1016/0027-5107(89)90196-6]
11. Tan Dx, Chen Ld. Poeggeller B. Melatonin: a potent endogenous hydroxyl radical scavenger. Endocr J 1993; 1:57-60.
12. Allegra M, Reiter RJ, Tan DX. The chemistry of melatonin's interaction with reactive species, J Pineal Res 2003; 34:1. [DOI:10.1034/j.1600-079X.2003.02112.x]
13. Reiter RJ. Pineal Melatonin: Cell biology of its synthesis and its physiolosical interactions . Endocr Rev 1991; 12: 151-156. [DOI:10.1210/edrv-12-2-151]
14. Marshall KA, Reiter RJ, Poeggeler B, Aruoma OI, Halliwell B. Evaluation of the antioxidant activity of melatonin in vitro. Free Radic Biol Med 1996; 21:307-315. [DOI:10.1016/0891-5849(96)00046-9]
15. Turkoz Y, Celik O , Hascalik S, Cigremis Y, Hascalik M, Mizrak B, et al. Melatonin reduces torsion - detorsion injury in rat ovary: Biochemical And Histopathologic Evaluation. J .Pineal Res 2004; 37: 137-141. [DOI:10.1111/j.1600-079X.2004.00146.x]
16. Celik O, Turkoz Y, Hascalik S, Hascalik M, Cigremis Y, Mizrak B, et al. The protective effect of caffeic acid phenethyl ester on ischemia-reperfusion injury in rat ovary: European Journal of Obstetrics & Gynecology and Reproductive Biology 2004; 117: 183-188. [DOI:10.1016/j.ejogrb.2004.05.007]
17. Hascalik S, Celik O, Turkoz Y, Hascalik M, Cigremis Y, Mizrak B, et al. Resveratrol, a red wine constituent polyphenol, protects from ischemia-reperfusion damage of the ovaries. Gynecol Obstet Invest 2004; 57: 218-223. [DOI:10.1159/000076760]
18. Melchiorri D, Reiter RJ, Sewerynek E, Hara M, Chen I, Nistico G. Paraquat toxicity and oxidative damage reduction by melatonin. Biochem Biochem Pharmacol 1996; 51: 1095-1099. [DOI:10.1016/0006-2952(96)00055-X]
19. MC Cord JM. Oxygen - derived free radicals in post ischemic tissue injury. N Engl J Med 1985; 312:159 - 163. [DOI:10.1056/NEJM198501173120305]
20. Koltuksuz U , Ozen S , Vz E, Aydinc M , Karaman A , Gultek A, et al. Caffeic acid phenethyl ester prevents intestinal reperfusion injury in rats. J Pediatr Surg 1999; 34:1458-1462. [DOI:10.1016/S0022-3468(99)90103-3]
21. Suzuki S, Toledo - Pereyra LH, Rodriguez F, Lopez F. Role of kupffer cells in neutrophil activation and infiltration following total hepatic ischemia and reperfusion. Circ Shock 1994; 42:204-209.
22. Reiter RJ, Tan DX, Manchester LC, Qi W. Biochemical reactivity of melatonin with reactive oxygen and nitrogen species. Cell Biochem Biophys 2001; 34:237-256. [DOI:10.1385/CBB:34:2:237]
23. Tan Dx, Reiter RJ, Manchester LC. Chemical and physical properties and potential mechanisms: melatonin as a broad spectrum anti oxidant and free radical scavenser. Curr Top Med Chem 2002; 2:181-197. [DOI:10.2174/1568026023394443]
24. Reiter RJ, Guerrero JM, Garcia JJ, Acuna-Castroviejo D. Reactive oxygen intermediates, molecular damage, and aging relation to melatonin. Ann NY Acad Sci 1998; 854:410-424. [DOI:10.1111/j.1749-6632.1998.tb09920.x]
25. Okatani Y. Melatonin increases activities of glutathione peroxidase and super oxide dismutase in fetal rat brain. J Pineal Res 2000; 28:89-96. [DOI:10.1034/j.1600-079X.2001.280204.x]
26. Rodriguez C, Mayo JC , Sains RM, Antolin I, Herrera F, Martin V, et al. Regulation of anti oxidant enzymes: a signficant role for melatonin . J Pineal Res 2004; 36: 1-9 [DOI:10.1046/j.1600-079X.2003.00092.x]
27. Hara M, Abe M, Suzuki T, Rieter RJ. Tissue changes in glutathione metabolism and lipid peroxidation induced by swimming are partially prevented by melatonin. Pharmacol Toxicol 1996; 78:308-312. [DOI:10.1111/j.1600-0773.1996.tb01380.x]
28. Durmus O, Aricioglu A, Guven T, Oguz M, Yel M, Turkozkan N. The effects of allopurinol on the liver ultrastructure, reduced glutathione and lipid percxide levels during liver ischemia in guinea pigs. Gen Pharmacol 1994; 25:781-786. [DOI:10.1016/0306-3623(94)90260-7]
29. Bertuglia S, Marchiafava Pl, Colantuoni A. Melatonin prevents ischemia reperfusion injury in hamster cheek. Pouch Micro Circulation Cardiovasc Res 1996; 31:947-952. [DOI:10.1016/S0008-6363(96)00030-2]
30. Sewerynek E, Reiter RJ, Melchiorri D, Ortiz GG, Lewinski A. Oxidative damage in the liver induced by ischemia-reperfusion: Protective by melatonin .Hepato - Gasteroenterology 1996; 43:898-905.
31. Celik H, Ayar A,Tug N, Simsek M, Ozercan I, Cikim G, et al . Effects of melatonin on noncardiogenic pulmonary edema secondary to adnexial ischemia- reperfusion in guinea pig. Neuroendocrinol Lett 2002; 23:115-118.
32. Torri K, Uneyama H, Nishino H, Kondoh T. Melatonin suppresses cerebral edema caused by middle cerebral artery occlusion/reperfusion in rats assesed by magnetic resonance imaging. J Pineal Res 2004; 36: 18-24. [DOI:10.1046/j.1600-079X.2003.00097.x]
33. Lahiri OK, Ghosh C. Interactions between melatonin, reactive oxygen species, and nitric oxide. Ann Ny Acad Sci 1999; 893: 325-330. [DOI:10.1111/j.1749-6632.1999.tb07847.x]
34. Oktem F, Ozguner F, Yilmaz Hr, Uz E. Melatonin reduces urinary excretion of n-acetyl-beta-d-glucosaminidase, albumin and renal oxidative markers in diabetic rats. Clin Exp Pharmacol Physiol 2006; 33:95-101. [DOI:10.1111/j.1440-1681.2006.04330.x]
35. Ozacmak VH, Sayan H, Arslan SO, Altaner S, Aktas RG. Protective effect of melatonin on contractile activity and oxidative injury induced by ischemia and reperfusion of rat ileum. Life Sci 2005; 76:1575-1588. [DOI:10.1016/j.lfs.2004.08.031]
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