Volume 22, Issue 8 (August 2024)                   IJRM 2024, 22(8): 627-638 | Back to browse issues page

Ethics code: IR.TUMS.MEDICINE.REC.1400.957


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Asadi B, Rakhshan K, Ranjbaran M, Abdi A, Vaziripour M, Seifi B. Carbon monoxide refines ovarian structure changes and attenuates oxidative stress via modulating of heme oxygenase system in a rat model of polycystic ovary syndrome: An experimental study. IJRM 2024; 22 (8) :627-638
URL: http://ijrm.ir/article-1-3229-en.html
1- Physiology Department, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
2- Physiology Department, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. , b-seifi@tums.ac.ir
Abstract:   (296 Views)
Background: Carbon monoxide (CO), influences ovarian function, pregnancy, and placental health. Heme oxygenase (HO)-1 and its products, including CO, exhibit protective and anti-inflammatory properties.
Objective: This study investigate the protective effects of CO released by the carbon dioxide-releasing molecule (CORM)-2 against oxidative stress, functional and structural changes of the ovaries, and HO-1 expressions in female rats suffering from polycystic ovary syndrome (PCOS).
Materials and Methods: In this experimental study, 24 Rattus norvegicus var. Albinus female rats (180-200 gr, 8 wk) were randomly divided into 4 groups (n = 6/each): control, CORM-2 (10 mg/kg), PCOS (induced by 4 mg/kg, intramuscular injection and a single dose of estradiol valerate), PCOS + CORM-2. Ovary histological changes were evaluated by crystal violet staining. Malondialdehyde (MDA) level and superoxide dismutase (SOD) activity of ovarian tissue were assessed using enzyme-linked immunosorbent assay. HO-1 expression was evaluated using Western blot.
Results: Corpus luteal formation significantly decreased in the PCOS group and was significantly restored with CORM-2 administration compared to the control group (p < 0.05). The expression of ovarian HO-1 protein was reduced in the PCOS group compared to controls (p < 0.01), and administration of CORM in PCOS rats significantly increased its expression (p < 0.0001). In addition, CORM administration markedly reduced ovarian MDA levels and restored SOD activity (p < 0.0001).
Conclusion: CORM-2 administration to PCOS rats created protective effects by reducing oxidative stress (reducing MDA level and restoring SOD activity) and increasing ovarian HO-1 protein.
Full-Text [PDF 1613 kb]   (318 Downloads)    
Type of Study: Original Article | Subject: Reproductive Biology

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb