Volume 22, Issue 8 (August 2024)                   IJRM 2024, 22(8): 627-638 | Back to browse issues page

Ethics code: IR.TUMS.MEDICINE.REC.1400.957

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Asadi B, Rakhshan K, Ranjbaran M, Abdi A, Vaziripour M, Seifi B. Carbon monoxide refines ovarian structure changes and attenuates oxidative stress via modulating of heme oxygenase system in a rat model of polycystic ovary syndrome: An experimental study. IJRM 2024; 22 (8) :627-638
URL: http://ijrm.ir/article-1-3229-en.html
Carbon monoxide refines ovarian structure changes and attenuates oxidative stress via modulating of heme oxygenase system in a rat model of polycystic ovary syndrome: An experimental study
Bahareh Asadi1 , Kamran Rakhshan1 , Mina Ranjbaran1 , Arash Abdi1 , Maryam Vaziripour1 , Behjat Seifi * 2
1- Physiology Department, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
2- Physiology Department, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. , b-seifi@tums.ac.ir
Abstract:   (338 Views)
Background: Carbon monoxide (CO), influences ovarian function, pregnancy, and placental health. Heme oxygenase (HO)-1 and its products, including CO, exhibit protective and anti-inflammatory properties.
Objective: This study investigate the protective effects of CO released by the carbon dioxide-releasing molecule (CORM)-2 against oxidative stress, functional and structural changes of the ovaries, and HO-1 expressions in female rats suffering from polycystic ovary syndrome (PCOS).
Materials and Methods: In this experimental study, 24 Rattus norvegicus var. Albinus female rats (180-200 gr, 8 wk) were randomly divided into 4 groups (n = 6/each): control, CORM-2 (10 mg/kg), PCOS (induced by 4 mg/kg, intramuscular injection and a single dose of estradiol valerate), PCOS + CORM-2. Ovary histological changes were evaluated by crystal violet staining. Malondialdehyde (MDA) level and superoxide dismutase (SOD) activity of ovarian tissue were assessed using enzyme-linked immunosorbent assay. HO-1 expression was evaluated using Western blot.
Results: Corpus luteal formation significantly decreased in the PCOS group and was significantly restored with CORM-2 administration compared to the control group (p < 0.05). The expression of ovarian HO-1 protein was reduced in the PCOS group compared to controls (p < 0.01), and administration of CORM in PCOS rats significantly increased its expression (p < 0.0001). In addition, CORM administration markedly reduced ovarian MDA levels and restored SOD activity (p < 0.0001).
Conclusion: CORM-2 administration to PCOS rats created protective effects by reducing oxidative stress (reducing MDA level and restoring SOD activity) and increasing ovarian HO-1 protein.
Keywords: PCOS, Rat, Oxidative stress, Heme oxygenase, CORM.
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Type of Study: Original Article | Subject: Reproductive Biology
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