Volume 18, Issue 6 (June 2020)                   IJRM 2020, 18(6): 425-438 | Back to browse issues page

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Darbandi S, Darbandi M, Agarwal A, Khorram Khorshid H R, Sadeghi M R, C.Esteves S, et al . Comparing four laboratory three-parent techniques to construct human aged non-surrounded nucleolus germinal vesicle oocytes: A case-control study. IJRM 2020; 18 (6) :425-438
URL: http://ijrm.ir/article-1-1424-en.html
Comparing four laboratory three-parent techniques to construct human aged non-surrounded nucleolus germinal vesicle oocytes: A case-control study
Sara Darbandi1 , Mahsa Darbandi * 2, Ashok Agarwal3 , Hamid Reza Khorram Khorshid4 , Mohammad Reza Sadeghi5 , Sandro C.Esteves6 , Pallav Sengupta7 , Sulagna Dutta7 , Zohreh Fathi1 , Hojjat Zeraati8 , Mohammad Mehdi Akhondi1
1- Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
2- Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran. , mahsadarbandi@hotmail.com
3- American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, Ohio, 44195, USA.
4- Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.
5- Department of Embryology and Andrology, Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
6- Androfert, Andrology and Human Reproduction Clinic, Campinas, Brazil.
7- Department of Physiology, Faculty of Medicine, Mahsa University, Selangor, Malaysia.
8- Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Abstract:   (2754 Views)
Background: The three-parent assisted reproductive technique may increase oocyte competence.
Objective: In this case-control study, the suitability of germinal vesicle transfer (GVT), synchronous ooplasmic transfer (sOT), asynchronous ooplasmic transfer using cryopreserved MII oocyte (caOT), and asynchronous ooplasmic transfer using waste MII oocyte (waOT) for maturation of the human-aged non-surrounded nucleolus germinal vesicle-stage (NSN-GV) oocyte were investigated.
Materials and Methods: NSN-GV oocytes were subjected to four methods: group A (GVT), B (sOT), C (caOT) D (waOT), and E (Control). The fusion rates, MI, MII, ICSI observations and cleavage at 2-cell, 4-cell, and 8-cell stages were compared in the groups.
Results: In GVT, none of the oocytes fused. In sOT, all oocytes fused, 20 achieved the MI, 14 progressed to MII, 8 fertilized, 6 cleaved and 5, 4, and 3 achieved the 2-cells, 4-cells and 8-cells, respectively. In caOT, all oocytes fused and achieved the MI, 8 progressed to MII and fertilized, 6 cleaved and 6, 5, and 5 achieved the 2-cells, 4-cells, and 8-cells respectively. In waOT, all oocytes fused, 5 and 3 progressed to MI and MII, respectively, but only one fertilized, cleaved and reached a 4-cells stage. In group E, 6 and 2 oocytes progressed to MI and MII, respectively, and only one fertilized but arrested at the zygote stage. caOT had the highest survival rate when compared to sOT (p = 0.04), waOT (p = 0.002), and control (p = 0.001).
Conclusion: The caOT method was beneficial over sOT, waOT, and GVT in supplementing the developmental capacity of human-aged NSN-GV oocytes.
Keywords: Assisted reproductive techniques, In vitro oocyte maturation techniques, Nuclear transfer techniques, Oocytes, Oocyte donation.
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Type of Study: Original Article | Subject: Reproductive Biology
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