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Reproductive Biomedicine
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Volume 17, Issue 6 (June 2019 2019)
IJRM 2019, 17(6): 395-404 |
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Bakhteyari A, Zarrin Y, Nikpour P, Hosseiny Z S, Mostafavi F S, Eskandari N, et al . Diabetes mellitus increased integrins gene
expression in rat endometrium at the time of
embryo implantation. IJRM 2019; 17 (6) :395-404
URL: http://ijrm.ir/article-1-1557-en.html
URL: http://ijrm.ir/article-1-1557-en.html
Abbas Bakhteyari1 
, Yasaman Zarrin1 , Parvaneh Nikpour2 , Zeinab Sadat Hosseiny1 , Fatemah Sadat Mostafavi1 , Nahid Eskandari3 , Mohammad Matinfar4 , Roshanak Aboutorabi * 5
, Yasaman Zarrin1 , Parvaneh Nikpour2 , Zeinab Sadat Hosseiny1 , Fatemah Sadat Mostafavi1 , Nahid Eskandari3 , Mohammad Matinfar4 , Roshanak Aboutorabi * 5
1- Department of Anatomical Sciences, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
2- Department of Genetics and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
3- Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
4- Department of Internal Medicine Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
5- Department of Anatomical Sciences, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. , aboutorabi.r@gmail.com
2- Department of Genetics and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
3- Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
4- Department of Internal Medicine Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
5- Department of Anatomical Sciences, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. , aboutorabi.r@gmail.com
Abstract: (2698 Views)
Background: Diabetes mellitus deeply changes the genes expression of integrin (Itg) subunits in several cells and tissues such as monocytes, arterial endothelium, kidney glomerular cells, retina. Furthermore, hyperglycemia could impress and reduce the rate of successful assisted as well as non-assisted pregnancy. Endometrium undergoes thorough changes in normal menstrual cycle and the question is: What happens in the endometrium under diabetic condition?
Objective: The aim of the current study was to investigate the endometrial gene expression of α3, α4, αv, Itg β1 and β3 subunits in diabetic rat models at the time of embryo implantation.
Materials and Methods: Twenty-eight rats were randomly divided into 4 groups: control group, diabetic group, pioglitazone-treated group, and metformin-treated group. Realtime PCR was performed to determine changes in the expression of Itg α3, α4, αv, β1, and β3 genes in rat’s endometrium.
Results: The expression of all Itg subunits increased significantly in diabetic rats’ endometrium compared with control group. Treatment with pioglitazone significantly reduced the level of Itg subunits gene expression compared with diabetic rats. While metformin had a different effect on α3 and α4 and elevated these two subunits gene expression.
Conclusion: Diabetes mellitus significantly increased the expression of studied Itg subunits, therefore untreated diabetes could be potentially assumed as one of the preliminary elements in embryo implantation failure.
Objective: The aim of the current study was to investigate the endometrial gene expression of α3, α4, αv, Itg β1 and β3 subunits in diabetic rat models at the time of embryo implantation.
Materials and Methods: Twenty-eight rats were randomly divided into 4 groups: control group, diabetic group, pioglitazone-treated group, and metformin-treated group. Realtime PCR was performed to determine changes in the expression of Itg α3, α4, αv, β1, and β3 genes in rat’s endometrium.
Results: The expression of all Itg subunits increased significantly in diabetic rats’ endometrium compared with control group. Treatment with pioglitazone significantly reduced the level of Itg subunits gene expression compared with diabetic rats. While metformin had a different effect on α3 and α4 and elevated these two subunits gene expression.
Conclusion: Diabetes mellitus significantly increased the expression of studied Itg subunits, therefore untreated diabetes could be potentially assumed as one of the preliminary elements in embryo implantation failure.
Type of Study: Original Article |
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