Volume 19, Issue 7 (July 2021)                   IJRM 2021, 19(7): 589-598 | Back to browse issues page

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solgi T, Amiri I, Soleimani Asl S, Saidijam M, Mirzaei seresht B, Artimani T. Antiapoptotic and antioxidative effects of cerium oxide nanoparticles on the testicular tissues of streptozotocin-induced diabetic rats: An experimental study. IJRM 2021; 19 (7) :589-598
URL: http://ijrm.ir/article-1-1819-en.html
Antiapoptotic and antioxidative effects of cerium oxide nanoparticles on the testicular tissues of streptozotocin-induced diabetic rats: An experimental study
Torab Solgi1 , Iraj Amiri2 , Sara Soleimani Asl2 , Massoud Saidijam3 , Banafsheh Mirzaei seresht2 , Tayebe Artimani * 4
1- Department of Anatomy, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
2- Endometrium and Endometriosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
3- Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
4- Endometrium and Endometriosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran. , artimani@umsha.ac.ir
Abstract:   (1478 Views)
Background: Cerium dioxide nanoparticles (CNPs) due to the antidiabetic and antioxidant activities are proposed for the treatment of oxidative stress-associated diseases.
Objective: To examine the impact of CNPs on hyperglycemia-induced apoptosis and oxidative stress in the testis of diabetic rats.
Materials and Methods: Twenty-four male rats were divided into four groups (n = 6/each) as diabetic rats, CNPs group, diabetic + CNPs rats, and controls. The control group was fed only with mouse food and water. Rats became diabetic through receiving streptozotocin (STZ) 60 mg/kg. CNPs were given to the rats at a dose of 30 mg/kg daily for two weeks. Malondialdehyde and total thiol group (TTG) levels were measured using spectrofluorometer. Expression of b-cell lymphoma protein 2-associated X protein (BAX) and b-cell lymphoma protein 2 (Bcl-2) were investigated using quantitative real-time polymerase chain reaction. Western blot analysis was used to examine caspase 3 protein levels.
Results: The content of malondialdehyde significantly increased in the STZ-diabetic rats, while TTG levels demonstrated a remarkable decrease. Caspase-3, BAX, and BAX/Bcl-2 mRNA ratio raised significantly in the STZ-diabetic rats. On the other hand, Bcl-2 mRNA levels reduced in the testis of diabetic rats (p = 0.006). Intervention with CNPs caused a substantial increase in the TTG levels, while the malondialdehyde contents, caspase-3, BAX levels, as well as BAX/Bcl-2 mRNA ratio were considerably decreased following CNPs treatment. Administration of CNPs increased mRNA levels of Bcl-2 (p < 0.0001).
Conclusion: CNPs treatment attenuates testicular apoptosis and oxidative stress induced by diabetes. This nanoparticle might be suggested for the treatment of diabetes-associated reproductive disorders.
Keywords: Apoptosis, Nanoceria, Diabetes, Oxidative stress, Testis.
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Type of Study: Original Article | Subject: Reproductive Andrology
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