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Reproductive Biomedicine
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Volume 19, Issue 11 (November 2021)
IJRM 2021, 19(11): 987-996 |
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Moradi A, Ghasemian F, Mashayekhi F. The reaggregation of normal granulosa-cumulus cells and mouse oocytes with polycystic ovarian syndrome in vitro: An experimental study. IJRM 2021; 19 (11) :987-996
URL: http://ijrm.ir/article-1-1944-en.html
URL: http://ijrm.ir/article-1-1944-en.html
1- Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran.
2- Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran. , ghasemian.21@gmail.com
2- Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran. , ghasemian.21@gmail.com
Abstract: (1103 Views)
Background: The dialogue between oocytes and their surrounding cells plays a major role in the progress of oocyte meiosis and their developmental potential.
Objective: This study aimed to evaluate the effect of co-culture of normal granulosa-cumulus cells (GCCs) with oocytes from polycystic ovarian syndrome (PCOS) mice.
Materials and Methods: Normal GCCs were collected from 10 virgin adult Naval Medical Research Institute female mice (30-35 gr, 7-8 wk old), and were cultured in an alpha-minimum essential medium supplemented with 5% fetal calf serum for 24-48 hr (1×106 cells/well). Then, germinal-vesicle oocytes from PCOS mice were cultured in the presence of cultured normal GCCs (experimental group) and without GCCs (control group). The maturation rate and quality of the PCOS oocytes were examined by evaluating TFAM and Cx43 gene expression (qReal Time-PCR) and the connection among PCOS oocytes and normal GCCs after 24 hr of culture.
Results: The co-culture of normal GCCs and PCOS oocytes in the experimental group led to the formation of a complex called a PCOS oocyte-normal GCCs complex. The maturation rate of these complexes was significantly increased compared to that of the control group (p ≤ 0.001). A significant difference was also found in the expression of Cx43 (p ≤ 0.001) and TFAM (p < 0.05) genes in the experimental group compared with the control group. The connection between PCOS oocytes and normal GCCs was observed in the scanning electron microscope images.
Conclusions: Co-culture with normal GCCs improves the capacity of PCOS oocytes to enter meiosis, which may result in the promotion of assisted reproduction techniques.
Objective: This study aimed to evaluate the effect of co-culture of normal granulosa-cumulus cells (GCCs) with oocytes from polycystic ovarian syndrome (PCOS) mice.
Materials and Methods: Normal GCCs were collected from 10 virgin adult Naval Medical Research Institute female mice (30-35 gr, 7-8 wk old), and were cultured in an alpha-minimum essential medium supplemented with 5% fetal calf serum for 24-48 hr (1×106 cells/well). Then, germinal-vesicle oocytes from PCOS mice were cultured in the presence of cultured normal GCCs (experimental group) and without GCCs (control group). The maturation rate and quality of the PCOS oocytes were examined by evaluating TFAM and Cx43 gene expression (qReal Time-PCR) and the connection among PCOS oocytes and normal GCCs after 24 hr of culture.
Results: The co-culture of normal GCCs and PCOS oocytes in the experimental group led to the formation of a complex called a PCOS oocyte-normal GCCs complex. The maturation rate of these complexes was significantly increased compared to that of the control group (p ≤ 0.001). A significant difference was also found in the expression of Cx43 (p ≤ 0.001) and TFAM (p < 0.05) genes in the experimental group compared with the control group. The connection between PCOS oocytes and normal GCCs was observed in the scanning electron microscope images.
Conclusions: Co-culture with normal GCCs improves the capacity of PCOS oocytes to enter meiosis, which may result in the promotion of assisted reproduction techniques.
Type of Study: Original Article |
Subject:
Reproductive Biology
References
1. Azziz R. Polycystic ovary syndrome. Obstet Gynecol 2018; 132: 321-336. [DOI:10.1097/AOG.0000000000002698] [PMID]
2. Shah D, Rasool S. Polycystic ovary syndrome and metabolic syndrome: The worrisome twosome? Climacteric 2016; 19: 7-16. [DOI:10.3109/13697137.2015.1116505] [PMID]
3. Hassani F, Oryan S, Eftekhari-Yazdi P, Bazrgar M, Moini A, Nasiri N, et al. Association between the number of retrieved mature oocytes and insulin resistance or sensitivity in infertile women polycystic ovary syndrome. Int J Fertil Steril 2019; 12: 310-315.
4. Xi W, Yang Y, Mao H, Zhao X, Liu M, Fu S. Circulating anti-mullerian hormone as predictor of ovarian response to clomiphene citrate in women with polycystic ovary syndrome. J Ovarian Res 2016; 9: 3. [DOI:10.1186/s13048-016-0214-2] [PMID] [PMCID]
5. Palomba S, Daolio J, La Sala GB. Oocyte competence in women with polycystic ovary syndrome. Trends Endocrinol Metab 2017; 28: 186-198. [DOI:10.1016/j.tem.2016.11.008] [PMID]
6. Amer SA, Mahran A, Abdelmaged A, El-Adawy AR, Eissa MK, Shaw RW. The influence of circulating anti-Müllerian hormone on ovarian responsiveness to ovulation induction with gonadotrophins in women with polycystic ovarian syndrome: A pilot study. Reprod Biol Endocrinol 2013; 11: 115. [DOI:10.1186/1477-7827-11-115] [PMID] [PMCID]
7. Tahaei LS, Eimani H, Eftekhar-Yazdi P, Ebrahimi B, Fathi R. Effects of retinoic acid on maturation of immature mouse oocytes in the presence and absence of a granulosa cell co-culture system. J Assist Reprod Genet 2011; 28: 553-558. [DOI:10.1007/s10815-011-9579-8] [PMID] [PMCID]
8. Jafari Atrabi M, Akbarinejad V, Khanbabaee R, Dalman A, Amorim CA, Najar-Asl M, et al. Formation and activation induction of primordial follicles using granulosa and cumulus cells conditioned media. J Cell Physiol 2019; 234: 10148-10156. [DOI:10.1002/jcp.27681] [PMID]
9. Palmerini MG, Nottola SA, Tunjung WA, Kadowaki A, Bianchi S, Cecconi S, et al. EGF-FSH supplementation reduces apoptosis of pig granulosa cells in co-culture with cumulus-oocyte complexes. Biochem Biophys Res Commun 2016; 481: 159-164. [DOI:10.1016/j.bbrc.2016.10.151] [PMID]
10. Oi A, Tasaki H, Munakata Y, Shirasuna K, Kuwayama T, Iwata H. Effects of reaggregated granulosa cells and oocytes derived from early antral follicles on the properties of oocytes grown in vitro. J Reprod Dev 2015; 61: 191-197. [DOI:10.1262/jrd.2014-123] [PMID] [PMCID]
11. Sharma GT, Dubey PK, Nath A, Saikumar G. Co-culture of buffalo (Bubalus bubalis) preantral follicles with antral follicles: A comparative study of developmental competence of oocytes derived from in vivo developed and in vitro cultured antral follicles. Zygote 2013; 21: 286-294. [DOI:10.1017/S0967199411000700] [PMID]
12. Zhang Y, Xu Y, Kuai Y, Wang S, Xue Q, Shang J. Effect of testosterone on the Connexin37 of sexual mature mouse cumulus oocyte complex. J Ovarian Res 2016; 9: 82. [DOI:10.1186/s13048-016-0290-3] [PMID] [PMCID]
13. Russell DL, Robker RL. Molecular mechanisms of ovulation: Co-ordination through the cumulus complex. Hum Reprod Update 2007; 13: 289-312. [DOI:10.1093/humupd/dml062] [PMID]
14. Wu CH, Yang JG, Yang JJ, Lin YM, Tsai HD, Lin CY, et al. Androgen excess down-regulates connexin43 in a human granulosa cell line. Fertil Steril 2010; 94: 2938-2941. [DOI:10.1016/j.fertnstert.2010.06.077] [PMID]
15. Bahadori MH, Azarnia M, Ghasemian F. [The effect of hepatocyte growth factor on mouse oocyte in vitro maturation and subsequent fertilization and embryo development]. Zahedan J Res Med Sci 2011; 13: 26-30. (in Persian)
16. Cheraghi E, Soleimani Mehranjani M, Shariatzadeh SMA, Nasr Esfahani MH, Alani B. N-acetylcysteine compared to metformin, improves the expression profile of growth differentiation factor-9 and receptor tyrosine kinase c-Kit in the oocytes of patients with polycystic ovarian syndrome. Int J Fertil Steril 2018; 11: 270-278.
17. Li J, Luo W, Huang T, Gong Y. Growth differentiation factor 9 promotes follicle-stimulating hormone-induced progesterone production in chicken follicular granulosa cells. Gen Comp Endocrinol 2019; 276: 69-76. [DOI:10.1016/j.ygcen.2019.03.005] [PMID]
18. Yamamoto N, Christenson LK, McAllister JM, Strauss JF. Growth differentiation factor-9 inhibits 3'5'-adenosine monophosphate-stimulated steroidogenesis in human granulosa and theca cells. J Clin Endocrinol Metab 2002; 87: 2849-2856.
https://doi.org/10.1210/jcem.87.6.8551 [DOI:10.1210/jc.87.6.2849] [PMID]
19. Omar Farouk FN, Stott D, Vlad M. Mouse embryo co-culture with autologous cumulus cells and fetal development post-embryo transfer. Anim Sci J 2011; 82: 420-427. [DOI:10.1111/j.1740-0929.2010.00869.x] [PMID]
20. Sowinska N, Müller K, Niżański W, Jewgenow K. Mitochondrial characteristics in oocytes of the domestic cat (Felis catus) after in vitro maturation and vitrification. Reprod Domest Anim 2017; 52: 806-813.
https://doi.org/10.1111/rda.12895 [DOI:10.1111/rda.12982]
21. Mizumachi S, Aritomi T, Sasaki K, Matsubara K, Hirao Y. Macromolecular crowded conditions strengthen contacts between mouse oocytes and companion granulosa cells during in vitro growth. J Reprod Dev 2018; 64: 153-160.
https://doi.org/10.1262/jrd.2017-162 [DOI:10.1262/jrd.2017-162e] [PMID] [PMCID]
22. Lin YH, Hwang JL, Seow KM, Huang LW, Chen HJ, Tzeng CR. Effects of growth factors and granulosa cell co-culture on in-vitro maturation of oocytes. Reprod Biomed Online 2009; 19: 165-170. [DOI:10.1016/S1472-6483(10)60068-5]
23. Adeldust H, Zeinoaldini S, Kohram H, Amiri Roudbar M, Daliri Joupari M. In vitro maturation of ovine oocyte in a modified granulosa cells co-culture system and alpha-tocopherol supplementation: Effects on nuclear maturation and cleavage. J Anim Sci Technol 2015; 57: 27. [DOI:10.1186/s40781-015-0061-5] [PMID] [PMCID]
24. Sirard MA, Bilodeau S. Granulosa cells inhibit the resumption of meiosis in bovine oocytes in vitro. Biol Reprod 1990; 43: 777-783. [DOI:10.1095/biolreprod43.5.777] [PMID]
25. Ghaffari Novin M, Noruzinia M, Allahveisi A, Saremi A, Fadaei Fathabadi F, Mastery Farahani R, et al. Comparison of mitochondrial-related transcriptional levels of TFAM, NRF1 and MT-CO1 genes in single human oocytes at various stages of the oocyte maturation. Iran Biomed J 2015; 19: 23-28.
26. Carabatsos MJ, Sellitto C, Goodenough DA, Albertini DF. Oocyte-granulosa cell heterologous gap junctions are required for the coordination of nuclear and cytoplasmic meiotic competence. Dev Biol 2000; 226: 167-179. [DOI:10.1006/dbio.2000.9863] [PMID]
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