Volume 9, Issue 1 (7-2011)                   IJRM 2011, 9(1): 0-0 | Back to browse issues page

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Barati F, Papahn A A, Afrough M, Barati M. Effects of Tyrode's solution osmolarities and milk on bull sperm storage above zero temperatures. IJRM 2011; 9 (1)
URL: http://ijrm.ir/article-1-207-en.html
Effects of Tyrode's solution osmolarities and milk on bull sperm storage above zero temperatures
Farid Barati * 1, Ahmad Ali Papahn2 , Mahsa Afrough2 , Mohammad Barati3
1- Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran , fabrtir@yahoo.com, baratif@scu.ac.ir
2- Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3- Department of Applied Chemistry, University of Tabriz, Tabriz, Iran
Abstract:   (2562 Views)
Background: Control of the medium osmolarity and temperature during long or short time sperm manipulation is essential.
Objective: The objectives of the present study were to find the effects of different osmolarities of modified Tyrode's solution and milk on the bull sperm during incubation at above zero temperatures.
Materials and Methods: Semen samples were collected twice from five Najdi bull. Centrifuged and most parts of seminal plasma were removed. First experiment: The concentrated semen were splited into nine aliquots to incubate in three different osmolarities (200, 300 and 400 mOsm) at three temperatures (5, 25 and 39°C) for 15 and 60 min of incubation. Second experiment: The semen samples were splited, mixed with the same volume of whole cow milk (5 and 25°C) and milk with 7% glycerol (5°C) and incubated for 15 and 60 min.
Results: Sperm motility severely affected (p<0.05) by incubation at low ionic tension (200 mOsm/l) especially at low temperature (5°C). The impact of low osmolarity on sperm viability can reduce by increasing the incubation temperature to 39°C. The decreased sperm motility, which was induced by lowering osmolarity, was not improved (p>0.05) by increasing temperature during 1 h of incubation. Milk can protect the sperm viability and motility at cool conditions and there is no beneficial effect of glycerol in combination of milk on sperm incubation at above zero temperatures (p<0.05).
Conclusion: Iso- and hyper-osmotic solutions protect bull sperm motility and viability at 25 and 39°C, while milk can be used for protecting sperm at 5°C. 
Keywords:  Bull sperm, Tyrode's solution, Whole milk, Osmolarity, Glycerol
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Type of Study: Original Article |
References
1. Gao DO, Mazur P, Critser JK. Fundamental cryobiology of mammalian spermatozoa, In: Karow AM, Critser JK. (Eds.). Reproductive tissue banking: scientific priniciples, San Diego, USA: Academic press; 1997: 263-328. [DOI:10.1016/B978-012399770-8/50007-1]
2. Morris GJ, Faszer K, Green JE, Draper D, Grout BWW, Fonseca F. Rapidly cooled horse spermatozoa: Loss of viability is due to osmotic imbalance during thawing, not intracellular ice formation. Theriogenology 2007; 68:804-812. [DOI:10.1016/j.theriogenology.2007.06.009]
3. Niasari-Naslaji A, Mosaferi S, Bahmani N, Abarghani A, Gharahdaghi AA, Gerami A. Effect of lactose extender with different levels of osmolality and pH on the viability of Bactrian camel (Camelus bactrianus) spermatozoa. IJVR 2006; 7: 14-22.
4. Andrade AFCd, Celeghini ECC, Yonezawa LA, Spers A, Arruda RPd. In vitro efficiency of three extenders for rabbit semen. Braz J Vet Res Anim Sci 2008; 45: 33-39.
5. Rigby SL, Brinsko SP, Cochran M, Blanchard TL, Love CC, Varner DD. Advances in cooled semen technologies: seminal plasma and semen extender. Anim Reprod Sci 2001;68: 171-180. [DOI:10.1016/S0378-4320(01)00154-3]
6. Agca Y, Gilmore J, Byers M, Woods EJ, Liu J, Critser JK. Osmotic characteristics of mouse spermatozoa in the presence of extenders and sugars. Biol Reprod 2002; 67:1493-1501. [DOI:10.1095/biolreprod.102.005579]
7. Liu ZS, Foote RH. Osmotic effects on volume and motility of bull sperm exposed to membrane permeable and nonpermeable agents. Cryobiology 1998; 37:207-218. [DOI:10.1006/cryo.1998.2116]
8. Waterhouse KE, Gjeldnes A, Tverdal A, De Angelis PM, Farstad W, Haard M, Kommisrud E. Alterations of sperm DNA integrity during cryo preservation procedure and in vitro incubation of bull semen. Anim Reprod Sci 2010; 117: 34-42. [DOI:10.1016/j.anireprosci.2009.04.011]
9. Pommer AC, Rutllant J, Meyers SA. The role of osmotic resistance on equine spermatozoal function. Theriogenology 2002; 58: 1373-1384. [DOI:10.1016/S0093-691X(02)01039-7]
10. Chaveiro A, Liu J, Engel B, Critser JK, Woelders H. Significant variability among bulls in the sperm membrane permeability for water and glycerol: Possible implications for semen freezing protocols for individual males. Cryobiology 2006; 53:349-359. [DOI:10.1016/j.cryobiol.2006.08.005]
11. Varisli O, Uguz C, Agca C, Agca Y. Motility and acrosomal integrity comparisons between electro-ejaculated and epididymal ram sperm after exposure to a range of anisosmotic solutions, cryoprotective agents and low temperatures. Anim Reprod Sci 2009; 110: 256-268. [DOI:10.1016/j.anireprosci.2008.01.012]
12. Zhao YY, Buhr MM. Cryopreservation extenders affect calcium flux in bovine spermatozoa during a temperature challenge. J Androl 1995; 16: 278-285.
13. Vishwanath R, Shannon P. Storage of bovine semen in liquid and frozen state. Anim Reprod Sci 2000; 62: 23-53. [DOI:10.1016/S0378-4320(00)00153-6]
14. Cooper TG, Hellenkemper B. Method-Related Estimates of Sperm Vitality. J Androl 2009; 30: 214-218. [DOI:10.2164/jandrol.108.005819]
15. SAS. SAS/STAT User's Guide. Release 6.03 Edn., Statistical Analysis Systems Institute, Inc., Cary, NC.; 1996: 550-641.
16. Brackett BG, Oliphant G. Capacitation of rabbit spermatozoa in vitro. Biol Reprod 1975; 12:260-274. [DOI:10.1095/biolreprod12.2.260]
17. Webb GW, Arns MJ. Influence of modified Tyrode's media on motility of cold-stored stallion spermatozoa. J Equine Vet Sci 1995; 15: 441-444. [DOI:10.1016/S0737-0806(06)81836-8]
18. Parks JE. Hypothermia and mammalian gametes. In: Karow AM, Critser JK. (Eds.). Reproductive tissue banking: scientific principles, San Diego, USA: Academic press; 1997: 229-247. [DOI:10.1016/B978-012399770-8/50006-X]
19. Batellier F, Vidament M, Fauquant J, Duchamp G, Arnaud G, Yvon JM, Magistrini M. Advances in cooled semen technology. Anim Reprod Sci 2001; 68: 181-190. [DOI:10.1016/S0378-4320(01)00155-5]
20. Ball BA. Oxidative stress, osmotic stress and apoptosis: Impacts on sperm function and preservation in the horse. Anim Reprod Sci 2008; 107:257-267. [DOI:10.1016/j.anireprosci.2008.04.014]
21. Zhang BR, Buhr M, Kroetsch T, Leibo SP. Glycine betaine improves survival of fresh bovine spermatozoa. Reproduction Fertility Dev 2001; 13: 187-192. [DOI:10.1071/RD01006]
22. Zhou JB, Yue KZ, Luo MJ, Chang ZL, Liang H, Wang ZY, Tan JH. Effect of extenders and temperatures on sperm viability and fertilizing capacity of Harbin white boar semen during long-term liquid storage. AJAS 2004; 17: 1501-1508. [DOI:10.5713/ajas.2004.1501]
23. Li G, Saenz J, Godke RA, Devireddy RV. Effect of glycerol and cholesterol-loaded cyclodextrin on freezing-induced water loss in bovine spermatozoa. Reproduction 2006; 131: 875-886. [DOI:10.1530/rep.1.00995]
24. Van Thuan N, Wakayama S, Kishigami S, Wakayama T. New preservation method for mouse spermatozoa without freezing. Biol Reprod 2005; 72: 444-450. [DOI:10.1095/biolreprod.104.034678]
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