Volume 20, Issue 4 (April 2022)                   IJRM 2022, 20(4): 273-288 | Back to browse issues page


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Abedpour N, Ghorbanmehr N, Salehnia M. Lysophosphatidic acid supports the development of vitrified ovarian follicles by decreasing the incidence of cell death: An experimental study. IJRM 2022; 20 (4) :273-288
URL: http://ijrm.ir/article-1-2119-en.html
1- Anatomy Department, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Anatomy Department, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
2- Biotechnology Department, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.
3- Anatomy Department, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. , salehnim@modares.ac.ir
Abstract:   (1261 Views)
Background: Lysophosphatidic acid (LPA) contributes to follicular activation, oocyte maturation, in vitro fertilization, and embryo implantation.
Objective: This study was designed to evaluate the effects of LPA to improve the development of isolated follicles derived from whole mouse cultured vitrified ovaries.
Materials and Methods: In this experimental study, first, the 1-wk-old mouse ovaries in the non-vitrified and vitrified groups were cultured in the presence of 20 µM of LPA for 1 wk. Then, their isolated preantral follicles were cultured individually for 12 days in the presence or absence of 40 µM of LPA. The following evaluations were done for the cultured follicles: a viability test using Calcein AM staining, flow cytometry using annexin V/Pi, and analysis of the expression of genes by real-time reverse transcription polymerase chain reaction. The maturation rates of the oocytes were compared among groups and some of the released metaphase II oocytes were subjected to in vitro fertilization.
Results: In all LPA treated groups, the rates of survival and follicular development were higher, and the incidence of cell death and expression of pro-apoptotic genes were lower, than in the non-LPA supplemented groups (p = 0.035). There was no significant difference between the vitrified and non-vitrified groups regarding follicular or oocyte development, but the expression of Bad and LPA receptors genes was significantly altered in the vitrified LPA supplemented group in comparison with the non-vitrified LPA supplemented group (p = 0.028).
Conclusion: LPA improved the survival and developmental potential of the isolated follicles. Despite some alterations in the expression of apoptosis-related genes in the vitrified ovaries, LPA had positive effects on the survival and development of these follicles.
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Type of Study: Original Article | Subject: Reproductive Biology

References
1. Botigelli RC, Razza EM, Pioltine EM, Gouveia Nogueira MF. New approaches regarding the in vitro maturation of oocytes: Manipulating cyclic nucleotides and their partners in crime. JBRA Assist Reprod 2017; 21: 35-44. [DOI:10.5935/1518-0557.20170010] [PMID] [PMCID]
2. Kim YY, Tamadon A, Ku SY. Potential use of antiapoptotic proteins and noncoding RNAs for efficient in vitro follicular maturation and ovarian bioengineering. Tissue Eng Part B Rev 2017; 23: 142-158. [DOI:10.1089/ten.teb.2016.0156] [PMID]
3. Kong HS, Kim EJ, Youm HW, Kim SK, Lee JR, Suh ChS, et al. Improvement in ovarian tissue quality with supplementation of antifreeze protein during warming of vitrified mouse ovarian tissue. Yonsei Med J 2018; 59: 331-336. [DOI:10.3349/ymj.2018.59.2.331] [PMID] [PMCID]
4. Abdi Sh, Salehnia M, Hosseinkhani S. Evaluation of apoptosis in long-term culture of vitrified mouse whole ovaries. Res Vet Sci 2014; 96: 1-4. [DOI:10.1016/j.rvsc.2013.09.016] [PMID]
5. Lee J, Kong HS, Kim EJ, Youm HW, Lee JR, Suh ChS, et al. Ovarian injury during cryopreservation and transplantation in mice: A comparative study between cryoinjury and ischemic injury. Hum Reprod 2016; 31: 1827-1837. [DOI:10.1093/humrep/dew144] [PMID]
6. Silva JRV, van den Hurk R, Figueiredo JR. Ovarian follicle development in vitro and oocyte competence: Advances and challenges for farm animals. Domest Anim Endocrinol 2016; 55: 123-135. [DOI:10.1016/j.domaniend.2015.12.006] [PMID]
7. Youm HW, Lee JR, Lee J, Jee BC, Suh CS, Kim SH. Optimal vitrification protocol for mouse ovarian tissue cryopreservation: Effect of cryoprotective agents and in vitro culture on vitrified-warmed ovarian tissue survival. Hum Reprod 2014; 29: 720-730. [DOI:10.1093/humrep/det449] [PMID]
8. Salehnia M, Pajokh M, Ghorbanmehr N. Short term organ culture of mouse ovary in the medium supplemented with bone morphogenetic protein 15 and follicle stimulating hormone: A morphological, hormonal and molecular study. J Reprod Infertil 2016; 17: 199-207.
9. Veshkini A, Khadem AA, Mohammadi-Sangcheshmeh A, Asadi Alamouti A, Soleimani M, Gastal EL. Linolenic acid improves oocyte developmental competence and decreases apoptosis of in vitro-produced blastocysts in goat. Zygote 2016; 24: 537-548. [DOI:10.1017/S0967199415000507] [PMID]
10. Wocławek-Potocka I, Rawińska P, Kowalczyk-Zieba I, Boruszewska D, Sinderewicz E, Waśniewski T, et al. Lysophosphatidic acid (LPA) signaling in human and ruminant reproductive tract. Mediators Inflamm 2014; 2014: 649702. [DOI:10.1155/2014/649702] [PMID] [PMCID]
11. Woclawek-Potocka I, Kowalczyk-Zieba I, Tylingo M, Boruszewska D, Sinderewicz E, Skarzynski DJ. Effects of lysophopatidic acid on tumor necrosis factor α and interferon γ action in the bovine corpus luteum. Mol Cell Endocrinol 2013; 377: 103-111. [DOI:10.1016/j.mce.2013.07.005] [PMID]
12. Ye X. Lysophospholipid signaling in the function and pathology of the reproductive system. Hum Reprod Update 2008; 14: 519-536. [DOI:10.1093/humupd/dmn023] [PMID]
13. Tokumura A, Miyake M, Nishioka Y, Yamano S, Aono T, Fukuzawa K. Production of lysophosphatidic acids by lysophospholipase D in human follicular fluids of in vitro fertilization patients. Biol Reprod 1999; 61: 195-199. [DOI:10.1095/biolreprod61.1.195] [PMID]
14. Boruszewska D, Sinderewicz E, Kowalczyk-Zieba I, Grycmacher K, Woclawek-Potocka I. Studies on lysophosphatidic acid action during in vitro preimplantation embryo development. Domest Anim Endocrinol 2016; 54: 15-29. [DOI:10.1016/j.domaniend.2015.08.003] [PMID]
15. Yung YC, Stoddard NC, Chun J. LPA receptor signaling: Pharmacology, physiology, and pathophysiology. J Lipid Res 2014; 55: 1192-1214. [DOI:10.1194/jlr.R046458] [PMID] [PMCID]
16. Bandoh K, Aoki J, Hosono H, Kobayashi S, Kobayashi T, Murakami-Murofushi K, et al. Molecular cloning and characterization of a novel human G-protein-coupled receptor, EDG7, for lysophosphatidic acid. J Biol Chem 1999; 274: 27776-27785. [DOI:10.1074/jbc.274.39.27776] [PMID]
17. Komatsu J, Yamano Sh, Kuwahara A, Tokumura A, Irahara M. The signaling pathways linking to lysophosphatidic acid-promoted meiotic maturation in mice. Life Sci 2006; 79: 506-511. [DOI:10.1016/j.lfs.2006.01.028] [PMID]
18. Thomson FJ, Perkins L, Ahern D, Clark M. Identification and characterization of a lysophosphatidic acid receptor. Mol Pharmacol 1994; 45: 718-723.
19. Sinderewicz E, Grycmacher K, Boruszewska D, Kowalczyk-Zięba I, Staszkiewicz J, Ślężak T, et al. Bovine ovarian follicular growth and development correlate with lysophosphatidic acid expression. Theriogenology 2018; 106: 1-14. [DOI:10.1016/j.theriogenology.2017.09.027] [PMID]
20. Liszewska E, Reinaud P, Billon-Denis E, Dubois O, Robin P, Charpigny G. Lysophosphatidic acid signaling during embryo development in sheep: Involvement in prostaglandin synthesis. Endocrinology 2009; 150: 422-434. [DOI:10.1210/en.2008-0749] [PMID]
21. Liszewska E, Reinaud P, Dubois O, Charpigny G. Lysophosphatidic acid receptors in ovine uterus during estrous cycle and early pregnancy and their regulation by progesterone. Domest Anim Endocrinol 2012; 42: 31-42. [DOI:10.1016/j.domaniend.2011.08.003] [PMID]
22. Su YQ, Sugiura K, Wigglesworth K, O'Brien MJ, Affourtit JP, Pangas SA, et al. Oocyte regulation of metabolic cooperativity between mouse cumulus cells and oocytes: BMP15 and GDF9 control cholesterol biosynthesis in cumulus cells. Development 2008; 135: 111-121. [DOI:10.1242/dev.009068] [PMID]
23. Boruszewska D, Torres AC, Kowalczyk-Zieba I, Diniz P, Batista M, Lopes-da-Costa L, et al. The effect of lysophosphatidic acid during in vitro maturation of bovine oocytes: Embryonic development and mRNA abundances of genes involved in apoptosis and oocyte competence. Mediators Inflamm 2014; 2014: 670670. [DOI:10.1155/2014/670670] [PMID] [PMCID]
24. Boruszewska D, Sinderewicz E, Kowalczyk-Zieba I, Grycmacher K, Woclawek-Potocka I. The effect of lysophosphatidic acid during in vitro maturation of bovine cumulus-oocyte complexes: Cumulus expansion, glucose metabolism and expression of genes involved in the ovulatory cascade, oocyte and blastocyst competence. Reprod Biol Endocrinol 2015; 13: 44. [DOI:10.1186/s12958-015-0044-x] [PMID] [PMCID]
25. Hama K, Aoki J, Bandoh K, Inoue A, Endo T, Amano T, et al. Lysophosphatidic receptor, LPA3, is positively and negatively regulated by progesterone and estrogen in the mouse uterus. Life Sci 2006; 79: 1736-1740. [DOI:10.1016/j.lfs.2006.06.002] [PMID]
26. Hinokio K, Yamano Sh, Nakagawa K, Iraharaa M, Kamada M, Tokumura A, et al. Lysophosphatidic acid stimulates nuclear and cytoplasmic maturation of golden hamster immature oocytes in vitro via cumulus cells. Life Sci 2002; 70: 759-767. [DOI:10.1016/S0024-3205(01)01448-5]
27. Jo JW, Jee BCh, Suh ChS, Kim SH. Addition of lysophosphatidic acid to mouse oocyte maturation media can enhance fertilization and developmental competence. Hum Reprod 2014; 29: 234-241. [DOI:10.1093/humrep/det427] [PMID]
28. Zhang JY, Jiang Y, Lin T, Kang JW, Lee JE, Jin DI. Lysophosphatidic acid improves porcine oocyte maturation and embryo development in vitro. Mol Reprod Dev 2015; 82: 66-77. [DOI:10.1002/mrd.22447] [PMID]
29. Abedpour N, Salehnia M, Ghorbanmehr N. The effect of lysophosphatidic acid on the follicular development and on the expression of its receptors during in vitro culture of mouse. Vet Res Forum 2018; 9: 59-66.
30. Abedpour N, Salehnia M, Ghorbanmehr N. The effects of lysophosphatidic acid on the incidence of cell death in cultured vitrified and non-vitrified mouse ovarian tissue: Separation of necrosis and apoptosis border. Cell J 2018; 20: 403-411. [DOI:10.26226/morressier.5912d9e9d462b80292385f8e]
31. Dehghan M, Shahbazi Sh, Salehnia M. Lysophosphatidic acid alters the expression of apoptosis related genes and miR-22 in cultured and autotransplanted ovaries. Cell J 2021; 23: 584-592.
32. Mohammadi Z, Hayati Roodbari N, Parivar K, Salehnia M. Supplementation of culture media with lysophosphatidic acid improves the follicular development of human ovarian tissue after xenotransplantaion into the the back muscle of γ-irradiated mice. Cell J 2020; 22: 358-366.
33. Salehnia M, Abbasian Moghadam E, Rezazadeh Velojerdi M. Ultrastructure of follicles after vitrification of mouse ovarian tissue. Fertil Steril 2002; 78: 644-645. [DOI:10.1016/S0015-0282(02)03287-9]
34. Abdi S, Salehnia M, Hosseinkhani S. Quality of oocytes derived from vitrified ovarian follicles cultured in two- and three-dimensional culture system in the presence and absence of Kit ligand. Biopreserv Biobank 2016; 14: 279-288. [DOI:10.1089/bio.2015.0069] [PMID]
35. de Castro FC, Cruz MH, Leal CL. Role of growth differentiation factor 9 and bone morphogenetic protein 15 in ovarian function and their importance in mammalian female fertility. Asian-Australas J Anim Sci 2016; 29: 1065-1074. [DOI:10.5713/ajas.15.0797] [PMID] [PMCID]
36. Sinderewicz E, Grycmacher K, Boruszewska D, Kowalczyk-Zięba I, Staszkiewicz J, Ślężak T, et al. Expression of factors involved in apoptosis and cell survival is correlated with enzymes synthesizing lysophosphatidic acid and its receptors in granulosa cells originating from different types of bovine ovarian follicles. Reprod Biol Endocrinol 2017; 15: 72. https://doi.org/10.1186/s12958-017-0298-6 [DOI:10.1186/s12958-017-0287-9]
38. Aikawa Sh, Kano K, Inoue A, Aoki J. Proliferation of mouse endometrial stromal cells in culture is highly sensitive to lysophosphatidic acid signaling. Biochem Biophys Res Commun 2017; 484: 202-208. [DOI:10.1016/j.bbrc.2016.12.154] [PMID]
39. Torres AC, Boruszewska D, Batista M, Kowalczyk-Zieba I, Diniz P, Sinderewicz E, et al. Lysophosphatidic acid signaling in late cleavage and blastocyst stage bovine embryos. Mediatoes Inflamm 2014; 2014: 678968. [DOI:10.1155/2014/678968] [PMID] [PMCID]
40. Amoushahi M, Salehnia M, Mowla SJ, Ghorbanmehr N. Morphological and molecular aspects of in vitro culture of preantral follicles derived from vitrified ovarian. Cell J 2017; 19: 332-342.

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