Volume 20, Issue 10 (October 2022)                   IJRM 2022, 20(10): 861-872 | Back to browse issues page

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Mashayekhi P, Noruzinia M, Khodaverdi S. Metformin as a potential agent for modulating the faulty endometriotic mesenchymal stem cells: A case-control study. IJRM 2022; 20 (10) :861-872
URL: http://ijrm.ir/article-1-2209-en.html
1- Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
2- Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. , noruzinia@modares.ac.ir
3- Endometriosis Research Center, Iran University of Medical Sciences, Tehran, Iran.
Abstract:   (840 Views)
Background: According to stem cell theory, it seems that the proliferation/differentiation imbalance in endometrial mesenchymal stem cells (eMSCs) is the leading cause of endometriosis, so targeting them to modulate stemness-relevant factors seems to be a wise choice for endometriosis treatment.
Objective: We aimed to investigate the effects of metformin on stemness properties of eMSCs by evaluating the expression profile of stemness-related genes and microRNAs (miRNAs).
Materials and Methods: In this case-control study, MSCs were isolated from the eutopic endometrium of 3 endometriotic and 3 healthy women. After their characterization and culture, they were treated with 0.1, 1, and 10 mM metformin for 72 hr. Finally, the expression of octamer-binding transcription factor (OCT) 4A, OCT4B, OCT4B1, sex determining region Y-Box transcription factor 2, nanog homeobox, microRNA-200b, microRNA-145, and lethal-7b were analyzed by quantitative reverse transcription-polymerase chain reaction.
Results: Metformin modulated the expression of stemness-related genes and miRNAs, OCT4A, OCT4B, OCT4B1, sex determining region Y-Box transcription factor 2, nanog homeobox, microRNA-200b, microRNA-145, and lethal-7b in eMSCs, especially at 1 and 10 mM concentration. Notably, metformin had a paradoxical effect on normal eMSCs.
Conclusion: We showed that metformin could modulate the expression of deregulated genes and miRNAs in faulty eMSCs, and restore their skewed self-renewal/differentiation balance, so it might be a promising drug for endometriosis treatment. The paradoxical effect of metformin on eMSCs and normal eMSCs might be because of their different metabolic patterns, so it requires further investigation to illustrate.
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Type of Study: Original Article | Subject: Reproductive Genetics

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