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Volume 19, Issue 6 (June 2021)
IJRM 2021, 19(6): 577-578 |
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Kadir R E, Ibrahim A, Ibrahim B A, Gwadabe S M, Sulaiman-Jaji R, Adigun M F et al . Author's Reply: "Low-dose bitter leaf improves sperm quality disrupted in immunosuppressed Wistar rats: An experimental study". IJRM 2021; 19 (6) :577-578
URL: http://ijrm.ir/article-1-2296-en.html
URL: http://ijrm.ir/article-1-2296-en.html
Risikat Eniola Kadir1 
, Abdulmumin Ibrahim * 2, Balkis Abimbola Ibrahim1 , Sadiya Musa Gwadabe1 , Rukayat Sulaiman-Jaji1 , Munirat Foyeke Adigun1 , Adeoye Oyetunji Oyewopo1
, Abdulmumin Ibrahim * 2, Balkis Abimbola Ibrahim1 , Sadiya Musa Gwadabe1 , Rukayat Sulaiman-Jaji1 , Munirat Foyeke Adigun1 , Adeoye Oyetunji Oyewopo1
1- Department of Anatomy, Faculty of Basic Medical Sciences, University of Ilorin, Ilorin, PMB 1515, Nigeria.
2- Department of Anatomy, Faculty of Basic Medical Sciences, University of Ilorin, Ilorin, PMB 1515, Nigeria. Department of Human Biology, University of Cape Town, Cape Town, South Africa. ,ibrahim.am@unilorin.edu.ng
2- Department of Anatomy, Faculty of Basic Medical Sciences, University of Ilorin, Ilorin, PMB 1515, Nigeria. Department of Human Biology, University of Cape Town, Cape Town, South Africa. ,
Abstract: (1302 Views)
Thank you for the observations made, clarifications required as well as the suggestions.
We have gone through and our response is thus:
1) We understand that spermatogenesis cycle in rats lasts on an average for 56
days. We administered rats with prednisolone, then treated them with various doses
of Vernonia amygdalina, and a comparison was drawn between the groups and the
control. This comparison demonstrates the differences. We treated the rats for 21
days, which has shown to make a difference in their spermatogenesis parameters
when compared with the untreated group.. Similar studies with shorter duration of
administration have also shown difference in these sperm parameters, even with as
low as 14 days of treatment when compared to controls (1–3). In our study, the rats
were immunosuppressed with prednisolone, and then treated with low and high doses
of Vernonia amygdalina extract, to observe for the different possible effects. These
groupings were ideas conceived by the authors, which was part of the experimental
design of the study. Furthermore, studies have shown various administrations of similar
and higher doses being used (4, 5). According to the published data by Akah et al. and
Nwanjo, acute toxicity test in rats gave an LD50 of 1122 mg/kg as well as 1265.22 ± 56
mg/kg (6, 7).
We have gone through and our response is thus:
1) We understand that spermatogenesis cycle in rats lasts on an average for 56
days. We administered rats with prednisolone, then treated them with various doses
of Vernonia amygdalina, and a comparison was drawn between the groups and the
control. This comparison demonstrates the differences. We treated the rats for 21
days, which has shown to make a difference in their spermatogenesis parameters
when compared with the untreated group.. Similar studies with shorter duration of
administration have also shown difference in these sperm parameters, even with as
low as 14 days of treatment when compared to controls (1–3). In our study, the rats
were immunosuppressed with prednisolone, and then treated with low and high doses
of Vernonia amygdalina extract, to observe for the different possible effects. These
groupings were ideas conceived by the authors, which was part of the experimental
design of the study. Furthermore, studies have shown various administrations of similar
and higher doses being used (4, 5). According to the published data by Akah et al. and
Nwanjo, acute toxicity test in rats gave an LD50 of 1122 mg/kg as well as 1265.22 ± 56
mg/kg (6, 7).
Keywords: quality, immunosuppressed
Type of Study: Letter to Editor |
References
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2. Ebokaiwe AP, Ijomone OM, Osawe ShO, Chukwu ChJ, Ejike Ch ECC, Zhang G, et al. Alteration in sperm characteristics, endocrine balance and redox status in rats rendered diabetic by streptozotocin treatment: Attenuating role of Loranthusmicranthus. Redox Report 2018; 23: 194-205. [DOI:10.1080/13510002.2018.1540675] [PMID] [PMCID]
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5. Oluwatosin A, Bayo O, Oluchi A, Michael F. Protective effects of extracts of vernonia amygdalina, hibiscus sabdariffa and vitamin C against radiation-induced liver damage in rats. J Radiat Res 2008; 49: 123-131. [DOI:10.1269/jrr.07062] [PMID]
6. Akah PA, Okafor CL. Blood sugar lowering effect of Vernonia amygdalina Del, in an experimental rabbit model. Phytother Res 1992; 6: 171-173. [DOI:10.1002/ptr.2650060318]
7. Nwanjo HU. Efficacy of aqueous leaf extract of Vernonia amygdalina on plasma lipoprotein and oxidative status in diabetic rat models. Nigerian J Physiol Sci 2005; 20: 39-42.
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