Volume 20, Issue 10 (October 2022)                   IJRM 2022, 20(10): 831-840 | Back to browse issues page

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Aledani T H W, Al-Hayder M N, Al-Mayyahi R S. Severe deterioration in sperm parameters and testes of rats administered naproxen and diclofenac at pre-puberty: An experimental study. IJRM 2022; 20 (10) :831-840
URL: http://ijrm.ir/article-1-2352-en.html
Severe deterioration in sperm parameters and testes of rats administered naproxen and diclofenac at pre-puberty: An experimental study
Tamadir H. W. Aledani * 1, Manal N. Al-Hayder2 , Rawaa S. Al-Mayyahi3
1- Department of Clinical Laboratory Sciences, College of Pharmacy, University of Basrah, Basrah, Iraq. , tamadir.wadi@uobasrah.edu.iq
2- Department of Pharmacology and Toxicology, College of Pharmacy, University of Basrah, Basrah, Iraq.
3- Department of Clinical Laboratory Sciences, College of Pharmacy, University of Basrah, Basrah, Iraq.
Abstract:   (1734 Views)
Background: Although naproxen and diclofenac are the most commonly used nonsteroidal drugs, their toxicity affecting male reproductivity has not been sufficiently studied, particularly when used in pre-puberty.
Objective: This study aims to investigate the toxic effects of naproxen and diclofenac on sperm parameters and testes in pre-pubertal rats.
Materials and Methods: In this experimental study, 15 pre-pubertal male albino rats (aged 5 wk, weighted 70-80 gr) were used. The animals were divided in to 3 groups (n = 5/each) of control (0.1 ml dimethyl sulfoxide), naproxen (50 mg/kg), and diclofenac sodium (5 mg/kg), and were orally administered with these drugs every day for 3 wk. Epididymal spermatozoa were taken to assess sperm count, viability, and morphology. After preparing tissue sections, testicular histopathological and histomorphometric analyses were performed.
Results: The body weights of rats in both naproxen and diclofenac groups significantly decreased (p < 0.001 and p = 0.03, respectively) in comparison to the control group. Remarkably, the testis weight and total sperm number significantly decreased (p = 0.002, and p = 0.004 respectively) in the naproxen-administered rats only. The sperm viability percentage decreased significantly in both diclofenac and naproxen-administered groups (p ≤ 0.001 and p = 0.03 respectively). Moreover, there was a significant increase (p < 0.001) in the percentage of sperm morphological anomalies in both drug-administered groups. Also, the histological and morphometric findings exhibited severe histopathological appearances in the testes and seminiferous tubules parameters in both drug-administered groups.
Conclusion: Naproxen and diclofenac administrations of rats before their puberty induce considerable harm to sperm parameters and testicular histology and morphometry. These severe toxic effects can lead to potential infertility.
Keywords: Male infertility, Non-steroidal drugs, Pathology, Spermatozoa, Testis.
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Type of Study: Original Article | Subject: Reproductive Biology
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