Volume 20, Issue 10 (October 2022)                   IJRM 2022, 20(10): 851-860 | Back to browse issues page


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Rodríguez Gallego M V, Aparicio Prieto M V, Leza García A, Hernández Hernández J, Arizaleta Uralde J A, Casis Sáenz L. Metabolism of renin-angiotensin and enkephalin in human follicular fluid: An experimental study. IJRM 2022; 20 (10) :851-860
URL: http://ijrm.ssu.ac.ir/article-1-2354-en.html
1- Clínica Ginecológica Juana, Hernández, Logroño La Rioja Country, Spain. , marivirg80@gmail.com
2- Human Reproduction Unit, Cruces University Hospital, Barakaldo (Basque Country), Spain.
3- Clínica Ginecológica Juana, Hernández, Logroño La Rioja Country, Spain.
4- Department of Physiology, Faculty of Medicine and Nursing, University of the Basque Country (UPV/EHU), Bilbao, Spain.
Abstract:   (288 Views)
Background: The relationship between the biochemical characteristics of follicular fluid (FF), oocyte quality and embryonic development has not yet been elucidated. We compared samples of FF with a normal metabolic profile against samples with metabolic abnormalities to identify potential predictive biomarkers of reproductive success.
Objective: To analyze peptide activity in the FF of women undergoing in vitro fertilization using 3 samples of FF per individual.
Materials and Methods: FF samples were obtained by ovum pick-up. Pathological samples were defined as samples of FF obtained from women with a gynecological condition or with infertility. A total of 30 women participated in this study. 3 samples of FF were obtained per individual (90 samples), but 8 samples were excluded because they were hemolyzed. The samples (n = 82 FF) included controls (n = 36, donors without fertility problems), women with endometriosis (n = 15), unexplained infertility (n = 19), and aged > 39 (n = 12). We assessed local encephalinergics: aminopeptidase-N (puromycin sensitive aminopeptidase and neutral endopeptidase; and components of the angiotensin system of the reproductive tract: prolyl-endopeptidase, APN, aspartate-aminopeptidase, and basic-aminopeptidase.
Results: No differences were observed in peptide metabolism based on the presence or absence of oocytes in the FF. Women with endometriosis and aged > 39 yr showed alterations in puromycin sensitive aminopeptidase (p = 0.01), aminopeptidase-B (p = 0.01), aspartate-aminopeptidase (p < 0.001) and neutral endopeptidase (p < 0.001).
Conclusion: This study reveals alterations in the metabolism of enkephalin and angiotensin in pathological FF, which points to these components as potential diagnostic biomarkers.
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Type of Study: Original Article | Subject: Reproductive Physiology

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