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Volume 20, Issue 12 (December 2022)
IJRM 2022, 20(12): 1029-1038 |
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Javanmard F, Bidmeshkipour A, Ghasemian Yadegari J. Ceratonia siliqua L improved cryodamage of asthenozoospermic specimens: An experimental study. IJRM 2022; 20 (12) :1029-1038
URL: http://ijrm.ir/article-1-2572-en.html
URL: http://ijrm.ir/article-1-2572-en.html
1- Department of Biology, Faculty of Basic Science, Razi University, Kermanshah, Iran.
2- Department of Biology, Faculty of Basic Science, Razi University, Kermanshah, Iran. , abidmeshki@razi.ac.ir
3- Department of Pharmacognosy, School of Pharmacy, Lorestan University of Medical Sciences, Khorramabad, Iran
2- Department of Biology, Faculty of Basic Science, Razi University, Kermanshah, Iran. , abidmeshki@razi.ac.ir
3- Department of Pharmacognosy, School of Pharmacy, Lorestan University of Medical Sciences, Khorramabad, Iran
Abstract: (660 Views)
Abstract
Background: Sperm freezing is an important procedure in assisted reproductive technology. Freezing results in physical and chemical changes in the sperm. Ceratonia siliqua L (C.siliqua) is a tree that has antioxidant properties.
Objective: This study aimed to investigate the effect of different concentrations of C.siliqua in a freezing medium on semen parameters, and some biochemical parameters in asthenozoospermic specimens.
Materials and Methods: 40 asthenozoospermic specimens (semen specimens with motility < 32%) were obtained from men aged between 20-40 yr according to the World Health Organization criteria. Each sample was divided into 6 groups: I) fresh, II) control, III) 5, IV) 10, V) 20, and VI) 30 µg/ml C.siliqua extract were added to a freezing medium respectively. Then sperm parameters, malondialdehyde, total antioxidant capacity, reactive oxygen species, and sperm DNA assay were evaluated using related protocols after thawing.
Results: Data analysis shows that sperm parameter, and total antioxidant capacity level increased at a concentration of 20 µg/ml of C. siliqua extract compared to the other concentrations of C.siliqua extract after cryopreservation and thawing (p < 0.001). Also, the sperm DNA fragmentation assay, reactive oxygen species, and malondialdehyde levels were significantly reduced by adding 20 µg/ml of C. siliqua extract to the sperm freezing medium compared to the other treated groups after cryopreservation (p < 0.001).
Conclusion: C.siliqua extract significantly improved sperm parameters after cryopreservation and thawing in asthenozoospermic specimens, and the greatest impact was observed at the 20 µg/ml C.siliqua L extract concentration (p < 0.001).
Background: Sperm freezing is an important procedure in assisted reproductive technology. Freezing results in physical and chemical changes in the sperm. Ceratonia siliqua L (C.siliqua) is a tree that has antioxidant properties.
Objective: This study aimed to investigate the effect of different concentrations of C.siliqua in a freezing medium on semen parameters, and some biochemical parameters in asthenozoospermic specimens.
Materials and Methods: 40 asthenozoospermic specimens (semen specimens with motility < 32%) were obtained from men aged between 20-40 yr according to the World Health Organization criteria. Each sample was divided into 6 groups: I) fresh, II) control, III) 5, IV) 10, V) 20, and VI) 30 µg/ml C.siliqua extract were added to a freezing medium respectively. Then sperm parameters, malondialdehyde, total antioxidant capacity, reactive oxygen species, and sperm DNA assay were evaluated using related protocols after thawing.
Results: Data analysis shows that sperm parameter, and total antioxidant capacity level increased at a concentration of 20 µg/ml of C. siliqua extract compared to the other concentrations of C.siliqua extract after cryopreservation and thawing (p < 0.001). Also, the sperm DNA fragmentation assay, reactive oxygen species, and malondialdehyde levels were significantly reduced by adding 20 µg/ml of C. siliqua extract to the sperm freezing medium compared to the other treated groups after cryopreservation (p < 0.001).
Conclusion: C.siliqua extract significantly improved sperm parameters after cryopreservation and thawing in asthenozoospermic specimens, and the greatest impact was observed at the 20 µg/ml C.siliqua L extract concentration (p < 0.001).
Keywords: Asthenozoospermia, Ceratonia, Cryopreservation, Fertility preservation, Infertility, Male.Infertility.
Type of Study: Original Article |
Subject:
Fertility & Infertility
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