International Journal of
Reproductive Biomedicine
Tue, May 13, 2025
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Volume 19, Issue 5 (Suppl- 2021)
IJRM 2021, 19(5): 118-118 |
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Akbari A, Kazemi M, Aboutorabi R, Mostafavi F. O-17 Expression of CALM1, PSMD6, and AK124742 LncRNA genes in cumulus cells of infertile PCO women: A good predictor of successful fertilization. IJRM 2021; 19 (5) :118-118
URL: http://ijrm.ir/article-1-2949-en.html
URL: http://ijrm.ir/article-1-2949-en.html
1- Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
2- Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
3- Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. ,fs.mostafavi@gmail.com
2- Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
3- Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. ,
Abstract: (252 Views)
Background: In human assisted reproductive technology (ART), selection of high-quality embryos to transfer usually is based on morphological criteria but it cannot be always a good predictor of successful fertilization. Analyzing gene expression of cumulus cells (CCs) might lead to some important molecular information about the embryo quality. Calmodulin 1 (CALM1), Proteasome 26S Subunit, Non-ATPase 6 (PSMD6), and AK124742 expression in the CCs of pregnant patients were more significant compared to the non-pregnant ones. One of the well-known causes of female infertility is polycystic ovary syndrome (PCOS) and the number of retrieved oocytes with a higher implantation potential is limited, so the process of selecting good embryos in PCOS patients is very important.
Objective: The aim of this study was to compare the expression of CALM1, PSMD6, and AK124742 genes in the CCs of infertile PCO patients with control fertile group.
Materials and Methods: Samples were the CCs from 33 fertile egg donor women and 33 infertile PCO women. They undergo ART and the CCs were collected and frizzed till real time PCR (RT-PCR) was performed. The expression of CALM1, PSMD6, and AK124742 genes was detected by RT-PCR. Chemical pregnancy rates were used to assess the success of ART.
Results: Clinical pregnancy was observed in 38 of the 66 patients. Expression of all three genes CALM1, PSMD6, and AK124742 in the pregnant group were higher than the non-pregnant group. This increase was not significant for the CALM1 gene but for two genes PSMD6 (p < 0.001) and AK124742 (p < 0.05) were significant. The expression of CALM1 and ak124274 gene increased significantly and the expression of psmd6 decreased significantly in PCOs group compared to the control group (p < 0.05).
Conclusion: All three genes are proper markers for predicting embryo competence due to increased expression levels in pregnant groups.
Objective: The aim of this study was to compare the expression of CALM1, PSMD6, and AK124742 genes in the CCs of infertile PCO patients with control fertile group.
Materials and Methods: Samples were the CCs from 33 fertile egg donor women and 33 infertile PCO women. They undergo ART and the CCs were collected and frizzed till real time PCR (RT-PCR) was performed. The expression of CALM1, PSMD6, and AK124742 genes was detected by RT-PCR. Chemical pregnancy rates were used to assess the success of ART.
Results: Clinical pregnancy was observed in 38 of the 66 patients. Expression of all three genes CALM1, PSMD6, and AK124742 in the pregnant group were higher than the non-pregnant group. This increase was not significant for the CALM1 gene but for two genes PSMD6 (p < 0.001) and AK124742 (p < 0.05) were significant. The expression of CALM1 and ak124274 gene increased significantly and the expression of psmd6 decreased significantly in PCOs group compared to the control group (p < 0.05).
Conclusion: All three genes are proper markers for predicting embryo competence due to increased expression levels in pregnant groups.
Type of Study: Congress Abstract |
Subject:
Pregnancy Health
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