شنبه 1 شهریور 1404
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دوره 19، شماره 5 - ( 3-1400 )
جلد 19 شماره 5 صفحات 118-118 |
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Akbari A, Kazemi M, Aboutorabi R, Mostafavi F. O-17 Expression of CALM1, PSMD6, and AK124742 LncRNA genes in cumulus cells of infertile PCO women: A good predictor of successful fertilization. IJRM 2021; 19 (5) :118-118
URL: http://ijrm.ir/article-1-2949-fa.html
URL: http://ijrm.ir/article-1-2949-fa.html
O-17 Expression of CALM1, PSMD6, and AK124742 LncRNA genes in cumulus cells of infertile PCO women: A good predictor of successful fertilization. International Journal of Reproductive BioMedicine. 1400; 19 (5) :118-118
چکیده: (373 مشاهده)
Background: In human assisted reproductive technology (ART), selection of high-quality embryos to transfer usually is based on morphological criteria but it cannot be always a good predictor of successful fertilization. Analyzing gene expression of cumulus cells (CCs) might lead to some important molecular information about the embryo quality. Calmodulin 1 (CALM1), Proteasome 26S Subunit, Non-ATPase 6 (PSMD6), and AK124742 expression in the CCs of pregnant patients were more significant compared to the non-pregnant ones. One of the well-known causes of female infertility is polycystic ovary syndrome (PCOS) and the number of retrieved oocytes with a higher implantation potential is limited, so the process of selecting good embryos in PCOS patients is very important.
Objective: The aim of this study was to compare the expression of CALM1, PSMD6, and AK124742 genes in the CCs of infertile PCO patients with control fertile group.
Materials and Methods: Samples were the CCs from 33 fertile egg donor women and 33 infertile PCO women. They undergo ART and the CCs were collected and frizzed till real time PCR (RT-PCR) was performed. The expression of CALM1, PSMD6, and AK124742 genes was detected by RT-PCR. Chemical pregnancy rates were used to assess the success of ART.
Results: Clinical pregnancy was observed in 38 of the 66 patients. Expression of all three genes CALM1, PSMD6, and AK124742 in the pregnant group were higher than the non-pregnant group. This increase was not significant for the CALM1 gene but for two genes PSMD6 (p < 0.001) and AK124742 (p < 0.05) were significant. The expression of CALM1 and ak124274 gene increased significantly and the expression of psmd6 decreased significantly in PCOs group compared to the control group (p < 0.05).
Conclusion: All three genes are proper markers for predicting embryo competence due to increased expression levels in pregnant groups.
Objective: The aim of this study was to compare the expression of CALM1, PSMD6, and AK124742 genes in the CCs of infertile PCO patients with control fertile group.
Materials and Methods: Samples were the CCs from 33 fertile egg donor women and 33 infertile PCO women. They undergo ART and the CCs were collected and frizzed till real time PCR (RT-PCR) was performed. The expression of CALM1, PSMD6, and AK124742 genes was detected by RT-PCR. Chemical pregnancy rates were used to assess the success of ART.
Results: Clinical pregnancy was observed in 38 of the 66 patients. Expression of all three genes CALM1, PSMD6, and AK124742 in the pregnant group were higher than the non-pregnant group. This increase was not significant for the CALM1 gene but for two genes PSMD6 (p < 0.001) and AK124742 (p < 0.05) were significant. The expression of CALM1 and ak124274 gene increased significantly and the expression of psmd6 decreased significantly in PCOs group compared to the control group (p < 0.05).
Conclusion: All three genes are proper markers for predicting embryo competence due to increased expression levels in pregnant groups.
نوع مطالعه: Congress Abstract |
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