International Journal of
Reproductive Biomedicine
Wed, May 14, 2025
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Volume 19, Issue 5 (Suppl- 2021)
IJRM 2021, 19(5): 315-315 |
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Akhlaghi M, Ansari K, Tajgardoon M, Zarezadeh M, Ebrahimpour M, Haghirosadat BF. P-148 Synthesis and optimization of cationic liposomal system for miRNA-15a loading in order to use in prostate cancer treatment. IJRM 2021; 19 (5) :315-315
URL: http://ijrm.ir/article-1-3058-en.html
URL: http://ijrm.ir/article-1-3058-en.html
1- Department of Biochemistry, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Nano-Biotech Foresight Company Biotechnology Campus, Science and Technology Park of Yazd, Yazd, Iran.
2- Nano-Biotech Foresight Company Biotechnology Campus, Science and Technology Park of Yazd, Yazd, Iran.
3- Medical Nanotechnology and Tissue Engineering Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
4- Faculty of Dentistry, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
5- Tissue Engineering Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
6- Department of Biochemistry, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Department of Advanced Medical Sciences and Technologies, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. ,fhaghirosadat@gmail.com
2- Nano-Biotech Foresight Company Biotechnology Campus, Science and Technology Park of Yazd, Yazd, Iran.
3- Medical Nanotechnology and Tissue Engineering Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
4- Faculty of Dentistry, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
5- Tissue Engineering Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
6- Department of Biochemistry, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Department of Advanced Medical Sciences and Technologies, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. ,
Abstract: (240 Views)
Background: Prostate cancer is the second most common cancer among men and the fifth leading cause of death in the world. Gene therapy is a new method for cancer treatment. Liposomes are known as carriers for gene delivery, but microRNA instability and poor translating are important challenges in miRNA delivery.
Objective: The aim of this study is to provide an optimized formulation of cationic liposomal system in order to delivery of miRNA-15a as an anti-tumor agent to prostate cancer cell line (PC3).
Materials and Methods: In this study, different formulations of the cationic liposomal system with different content of phospholipid (10, 20, 30, 40, 50%) and positive charge were synthesized, its size and charge were determined by Zeta-Sizer (DLS), then the cell viability percentage of PC3 prostate cancer cell line after treatment with various liposomal systems was evaluated.
Results: Based on the results of the DLS device, the particle size was below 150 nm and zeta potential was in the range of 0 to +15 mV. The MTT results determined that the viability percentage of cells were between 70 to 90%.
Conclusion: The optimal formulation with appropriate size, charge and cells viability percentage which could increase anti-cancer effects of miRNA-15a to PC3 cell line was selected for miRNA-15a delivery.
Keywords:
Objective: The aim of this study is to provide an optimized formulation of cationic liposomal system in order to delivery of miRNA-15a as an anti-tumor agent to prostate cancer cell line (PC3).
Materials and Methods: In this study, different formulations of the cationic liposomal system with different content of phospholipid (10, 20, 30, 40, 50%) and positive charge were synthesized, its size and charge were determined by Zeta-Sizer (DLS), then the cell viability percentage of PC3 prostate cancer cell line after treatment with various liposomal systems was evaluated.
Results: Based on the results of the DLS device, the particle size was below 150 nm and zeta potential was in the range of 0 to +15 mV. The MTT results determined that the viability percentage of cells were between 70 to 90%.
Conclusion: The optimal formulation with appropriate size, charge and cells viability percentage which could increase anti-cancer effects of miRNA-15a to PC3 cell line was selected for miRNA-15a delivery.
Keywords:
Type of Study: Congress Abstract |
Subject:
Reproductive Genetics
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