پنجشنبه 25 اردیبهشت 1404
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دوره 19، شماره 5 - ( 3-1400 )
جلد 19 شماره 5 صفحات 315-315 |
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Akhlaghi M, Ansari K, Tajgardoon M, Zarezadeh M, Ebrahimpour M, Haghirosadat BF. P-148 Synthesis and optimization of cationic liposomal system for miRNA-15a loading in order to use in prostate cancer treatment. IJRM 2021; 19 (5) :315-315
URL: http://ijrm.ir/article-1-3058-fa.html
URL: http://ijrm.ir/article-1-3058-fa.html
P-148 Synthesis and optimization of cationic liposomal system for miRNA-15a loading in order to use in prostate cancer treatment. International Journal of Reproductive BioMedicine. 1400; 19 (5) :315-315
چکیده: (241 مشاهده)
Background: Prostate cancer is the second most common cancer among men and the fifth leading cause of death in the world. Gene therapy is a new method for cancer treatment. Liposomes are known as carriers for gene delivery, but microRNA instability and poor translating are important challenges in miRNA delivery.
Objective: The aim of this study is to provide an optimized formulation of cationic liposomal system in order to delivery of miRNA-15a as an anti-tumor agent to prostate cancer cell line (PC3).
Materials and Methods: In this study, different formulations of the cationic liposomal system with different content of phospholipid (10, 20, 30, 40, 50%) and positive charge were synthesized, its size and charge were determined by Zeta-Sizer (DLS), then the cell viability percentage of PC3 prostate cancer cell line after treatment with various liposomal systems was evaluated.
Results: Based on the results of the DLS device, the particle size was below 150 nm and zeta potential was in the range of 0 to +15 mV. The MTT results determined that the viability percentage of cells were between 70 to 90%.
Conclusion: The optimal formulation with appropriate size, charge and cells viability percentage which could increase anti-cancer effects of miRNA-15a to PC3 cell line was selected for miRNA-15a delivery.
Keywords:
Objective: The aim of this study is to provide an optimized formulation of cationic liposomal system in order to delivery of miRNA-15a as an anti-tumor agent to prostate cancer cell line (PC3).
Materials and Methods: In this study, different formulations of the cationic liposomal system with different content of phospholipid (10, 20, 30, 40, 50%) and positive charge were synthesized, its size and charge were determined by Zeta-Sizer (DLS), then the cell viability percentage of PC3 prostate cancer cell line after treatment with various liposomal systems was evaluated.
Results: Based on the results of the DLS device, the particle size was below 150 nm and zeta potential was in the range of 0 to +15 mV. The MTT results determined that the viability percentage of cells were between 70 to 90%.
Conclusion: The optimal formulation with appropriate size, charge and cells viability percentage which could increase anti-cancer effects of miRNA-15a to PC3 cell line was selected for miRNA-15a delivery.
Keywords:
نوع مطالعه: Congress Abstract |
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