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Volume 21, Issue 7 (July 2023)
IJRM 2023, 21(7): 577-584 |
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Fallah Asl H, Jalali Mashayekhi F, Zendedel A, Baazm M. The time course of nod-like receptor family pyrin domain containing 3 inflammasome complex expressions in the testis tissue of an experimental varicocele rat model: An experimental study. IJRM 2023; 21 (7) :577-584
URL: http://ijrm.ir/article-1-3076-en.html
URL: http://ijrm.ir/article-1-3076-en.html
1- Students Research Committee, Arak University of Medical Sciences, Arak, Iran.
2- Department of Genetics and Biochemistry, School of Medicine, Arak University of Medical Sciences, Arak, Iran.
3- Institute of Neuroanatomy, Uniklinik RWTH Aachen, 52074 Aachen, Germany.
4- Department of Anatomy, School of Medicine, Arak University of Medical Sciences, Arak, Iran. Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran. ,dr.baazm@arakmu.ac.ir
2- Department of Genetics and Biochemistry, School of Medicine, Arak University of Medical Sciences, Arak, Iran.
3- Institute of Neuroanatomy, Uniklinik RWTH Aachen, 52074 Aachen, Germany.
4- Department of Anatomy, School of Medicine, Arak University of Medical Sciences, Arak, Iran. Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran. ,
Abstract: (542 Views)
Background: Varicocele is characterized by abnormal dilation of the testicular vein, which results in hypoxia, the accumulation of reactive oxygen species, and the production of proinflammatory cytokines. It seems that a group of cytosolic receptors named nod-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome, is activated and involved in the pathogenesis of varicocele.
Objective: We aim to determine the time course of NLRP3 inflammasome expression in the testis tissue following varicocele induction.
Materials and Methods: In this experimental study, 36 adult Wistar rats (8 wk, 200-250 gr) were used. For the varicocele induction, the left renal vein was partially ligated. The mRNA levels of NLRP3, apoptosis-associated speck-like protein containing a caspase recruitment domain, and caspase-1 were evaluated by real-time polymerase chain reaction at 1, 2, 4, 8, and 12 wk after varicocele induction.
Results: Results showed that the gene expression of NLRP3 inflammasome component including NLRP3, apoptosis-associated speck-like protein containing a caspase recruitment domain, and caspase-1 did not alter during week 1, 2, 4, and 8 after operation (p = 0.09). 12 wk after varicocele induction, gene expression levels were significantly up-regulated (p = 0.02).
Conclusion: Our data provides clear evidence that varicocele stimulates inflammasome activation in the testis tissue 12 wk after the operation, and this time is required for investigating NLRP3 activity in the varicocele rat model.
Objective: We aim to determine the time course of NLRP3 inflammasome expression in the testis tissue following varicocele induction.
Materials and Methods: In this experimental study, 36 adult Wistar rats (8 wk, 200-250 gr) were used. For the varicocele induction, the left renal vein was partially ligated. The mRNA levels of NLRP3, apoptosis-associated speck-like protein containing a caspase recruitment domain, and caspase-1 were evaluated by real-time polymerase chain reaction at 1, 2, 4, 8, and 12 wk after varicocele induction.
Results: Results showed that the gene expression of NLRP3 inflammasome component including NLRP3, apoptosis-associated speck-like protein containing a caspase recruitment domain, and caspase-1 did not alter during week 1, 2, 4, and 8 after operation (p = 0.09). 12 wk after varicocele induction, gene expression levels were significantly up-regulated (p = 0.02).
Conclusion: Our data provides clear evidence that varicocele stimulates inflammasome activation in the testis tissue 12 wk after the operation, and this time is required for investigating NLRP3 activity in the varicocele rat model.
Type of Study: Original Article |
Subject:
Reproductive Anatomy
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