International Journal of
Reproductive Biomedicine
Thu, Jan 30, 2025
[Archive]
Volume 11, Issue 10 (12-2013)
IJRM 2013, 11(10): 843-0 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Aflatoonian N, Pourmasumi S, Aflatoonian A, Eftekhar M. Duration of storage does not influence pregnancy outcome in cryopreserved human embryos. IJRM 2013; 11 (10) :843-0
URL: http://ijrm.ir/article-1-351-en.html
URL: http://ijrm.ir/article-1-351-en.html
1- Madar Hospital, Yazd, Iran
2- Research and Clinical Center for Infertility, Shahid Sadughi University of Medical Sciences, Yazd, Iran
3- Department of Obstetrics and Gynecology, Research and Clinical Center for Infertility, Shahid Sadughi University of Medical Sciences, Yazd, Iran
4- Department of Obstetrics and Gynecology, Research and Clinical Center for Infertility, Shahid Sadughi University of Medical Sciences, Yazd, Iran ,Eftekharmaryam@yahoo.com
2- Research and Clinical Center for Infertility, Shahid Sadughi University of Medical Sciences, Yazd, Iran
3- Department of Obstetrics and Gynecology, Research and Clinical Center for Infertility, Shahid Sadughi University of Medical Sciences, Yazd, Iran
4- Department of Obstetrics and Gynecology, Research and Clinical Center for Infertility, Shahid Sadughi University of Medical Sciences, Yazd, Iran ,
Abstract: (2699 Views)
Background: Cryopreservation of embryos has been an usual component of clinic in assisted reproductive technology (ART) programs. Recently the dramatic increase in cryobiology activity in the clinical centers has enhanced methods of freezing and improved vitrification protocols are being developed.
Objective: The aim of our study was to assess the effect of storage duration of frozen embryo on ART outcome.
Materials and Methods: In this retrospective study the data of 651 frozen-thawed embryo transfer cycles were assessed over a 36-months period. Our patients were categorized according to storage time of freeze. Group I: less than 90 days, Group II: between 90-365 days. Group III: between 365-730 days. Group IV: between 730-1095 days. Group V: more than 1095 days. Clinical pregnancy and implantation rate were defined and statistical analysis was performed using Student t-test and Chi-square.
Results: According to our finding patient’s mean age was 31.05±5.231 years (range, 18-53 years), and 1204 embryos were transferred .The mean storage duration was 296.72±301.82 days. The mean number of embryo transferred per cycle was similar between groups (p=0.224). According to our analysis clinical pregnancy rate per embryo transfer cycle was similar between groups (p=0.563).
Conclusion: Our results showed that duration of storage had no negative effects on implantation of cryopreserved embryos. In our literature review we found a little article In this context. However our study showed duration of freezing don’t have any negative effects on implantation and pregnancy outcome, but more studies are needed to evaluate long term effects of storage duration on babies were born by cryopreserved embryos.
Objective: The aim of our study was to assess the effect of storage duration of frozen embryo on ART outcome.
Materials and Methods: In this retrospective study the data of 651 frozen-thawed embryo transfer cycles were assessed over a 36-months period. Our patients were categorized according to storage time of freeze. Group I: less than 90 days, Group II: between 90-365 days. Group III: between 365-730 days. Group IV: between 730-1095 days. Group V: more than 1095 days. Clinical pregnancy and implantation rate were defined and statistical analysis was performed using Student t-test and Chi-square.
Results: According to our finding patient’s mean age was 31.05±5.231 years (range, 18-53 years), and 1204 embryos were transferred .The mean storage duration was 296.72±301.82 days. The mean number of embryo transferred per cycle was similar between groups (p=0.224). According to our analysis clinical pregnancy rate per embryo transfer cycle was similar between groups (p=0.563).
Conclusion: Our results showed that duration of storage had no negative effects on implantation of cryopreserved embryos. In our literature review we found a little article In this context. However our study showed duration of freezing don’t have any negative effects on implantation and pregnancy outcome, but more studies are needed to evaluate long term effects of storage duration on babies were born by cryopreserved embryos.
Type of Study: Original Article |
References
1. Davar R, Eftekhar M, Tayebi N. Transfer of cryopreserved-thawed embryos in a cycle using exogenous steroids with or without prior gonadotropihin-releasing hormone agonist. J Med Sci 2007; 7: 880-883. [DOI:10.3923/jms.2007.880.883]
2. D'Angelo A. Ovarian hyperstimulation syndrome prevention strategies: cryopreservation of all embryos. Semin Reprod Med 2010; 28: 513-518. [DOI:10.1055/s-0030-1265679]
3. Pattinson HA, Hignett M, Dunphy BC, Fleetham JA. Outcome of thaw embryo transfer after cryopreservation of all embryos in patients at risk of ovarian hyperstimulation syndrome. Fertil Steril 1994; 62: 1192-1196. [DOI:10.1016/S0015-0282(16)57184-2]
4. Imudia AN, Awonuga AO, Kaimal AJ, Wright DL, Styer AK, Toth TL. Elective cryopreservation of all embryos with subsequent cryothaw embryo transfer in patients at risk for ovarian hyperstimulation syndrome reduces the risk of adverse obstetric outcomes: a preliminary study. Fertil Steril 2012; 99: 168-173. [DOI:10.1016/j.fertnstert.2012.08.060]
5. Tanos V, Friedler S, Zajicek G, Neiger M, Lewin A, Schenker JG. The impact of endometrial preparation on implantation following cryopreserved-thawed-embryo transfer. Gynecol Obstet Invest 2010; 41: 227-231. [DOI:10.1159/000292274]
6. Dulioust E, Toyama K, Busnel MC, Moutier R, Carlier M, Marchaland C, et al. Long-term effects of embryo freezing in mice. Proc Nat Acad Sci 1995; 92: 589-593. [DOI:10.1073/pnas.92.2.589]
7. Saragusty J, Arav A. Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification. Reproduction 2011; 141: 1-19. [DOI:10.1530/REP-10-0236]
8. Auroux M, Cerutti I, Ducot B, Loeuillet A. Is embryo-cryopreservation really neutral?: A new long-term effect of embryo freezing in mice: protection of adults from induced cancer according to strain and sex. Reprod Toxicol 2004; 18: 813-818. [DOI:10.1016/j.reprotox.2004.04.010]
9. Aflatoonian A, Oskouian H, Ahmadi Sh, Oskouian L. Can fresh embryo transfers be replaced by cryopreserved-thawed embryo transfers in assisted reproductive cycles? A randomized controlled trial. J Assist Reprod Genet 2010; 27: 357-363. [DOI:10.1007/s10815-010-9412-9]
10. Dowling-Lacey D, Mayer JF, Jones E, Bocca S, Stadtmauer L, Oehninger S. Live birth from a frozen–thawed pronuclear stage embryo almost 20 years after its cryopreservation. Fertil Steril 2011; 95: 1120. [DOI:10.1016/j.fertnstert.2010.08.056]
11. AbdelHafez FF, Desai N, Abou-Setta AM, Falcone T, Goldfarb J. Slow freezing, vitrification and ultra-rapid freezing of human embryos: a systematic review and meta-analysis. Reprod Biomed Online 2010; 20: 209-222. [DOI:10.1016/j.rbmo.2009.11.013]
12. Testart J, Lassalle B, Forman R, Gazengel A, Belaisch-Allart J, Hazout A, et al. Factors influencing the success rate of human embryo freezing in an in vitro fertilization and embryo transfer program. Fertil Steril 1987; 48: 107-112. [DOI:10.1016/S0015-0282(16)59298-X]
13. Cohen J, et al. Duration of storage of cyropreserved human embryos. J In vitro Fert Embryo Transf 1988; 5: 301-303. [DOI:10.1007/BF01132183]
14. Ashrafi M, Jahangiri N, Hassani F, Akhoond MR, Madani T. The factors affecting the outcome of frozen-thawed embryo transfer cycle. Taiwan J Obstet Gynecol 2011; 50: 159-164. [DOI:10.1016/j.tjog.2011.01.037]
15. Wilson C, Check JH, Summers-Chase D, Choe JK, Amui J, Brasile D. Effect of the length of time that donated embryos are frozen on pregnancy outcome. Clin Exp Obstet Gynecol 2010; 37: 181-182.
16. Gozdecka DA. The Polish Catholic Church and the Regulation of IVF in Poland: Polarised Political Discourses and the Battle over 'Proper' Reproduction. feminists@ law [Internet]. 2012. available at: http://journals.kent.ac.uk/index.php/ feministsatlaw/article/view/26.
17. Leibovitz BE, Siegel BV. Aspects of free radical reactions in biological systems: aging. J Gerontol 1980; 35: 45-56. [DOI:10.1093/geronj/35.1.45]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
