International Journal of
Reproductive Biomedicine
Mon, Sep 29, 2025
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Volume 23, Issue 8 (August 2025)
IJRM 2025, 23(8): 637-646 |
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Ethics code: IR.SSU.AEC.1401.011
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Fotoohi-Ardakani G, Ghasemi N, Hassanpour Dehnavi A, Dadbinpour A, Lotfi M. Impact of platelet-rich plasma at different concentrations on genes of apoptotic pathway and differentiation of stem cells in cyclophosphamide-induced ovarian failure in a mouse model: An experimental study. IJRM 2025; 23 (8) :637-646
URL: http://ijrm.ir/article-1-3577-en.html
URL: http://ijrm.ir/article-1-3577-en.html
Gholamreza Fotoohi-Ardakani1 
, Nasrin Ghasemi2 , Azam Hassanpour Dehnavi3 , Ali Dadbinpour4 , Marzieh Lotfi *5
, Nasrin Ghasemi2 , Azam Hassanpour Dehnavi3 , Ali Dadbinpour4 , Marzieh Lotfi *5
1- Department of Molecular Medicine, School of Advanced Technologies in Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
2- Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
3- Department of Anatomical Sciences, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
4- Department of Medical Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. & Genetics and Environmental Hazards Research Center, Abarkouh School of Medical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
5- Department of Molecular Medicine, Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. ,M.lotfi@ssu.ac.ir
2- Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
3- Department of Anatomical Sciences, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
4- Department of Medical Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. & Genetics and Environmental Hazards Research Center, Abarkouh School of Medical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
5- Department of Molecular Medicine, Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. ,
Abstract: (89 Views)
Background: Platelet-rich plasma (PRP), a new therapeutic technique, has the potential to regenerate failing ovarian tissue and alleviate symptoms in patients with premature ovarian failure (POF).
Objective: This study examined the effect of different platelet concentrations on ovarian function, investigated the apoptosis genes B-cell lymphoma 2 (BCL-2) and Bcl-2-associated X protein, and the differentiation gene octamer-binding transcription factor 4.
Materials and Methods: In this experimental study, 30 female Syrian mice (8-10 wk, 25-30 gr) were randomly divided into 5 groups (n = 6/each): 1) normal control, 2) POF + phosphate buffered saline, 3) POF + PRP 0.25 ml/kg, 4) POF + PRP 0.5 ml/kg, and 5) POF + PRP 1 ml/kg. The cyclophosphamide was used to create a mouse model of POF. After 2 wk from injection of PRP and phosphate buffered saline, ovaries were removed for histological and molecular analysis.
Results: The distribution of different follicle types in the POF + PRP group was the same as that of the control group, according to a morphometric study. Bcl-2-associated X protein gene expression was significantly reduced in the groups that received PRP (p < 0.001). BCL-2 and octamer-binding transcription factor 4 gene expression was also increased in the PRP groups (p < 0.05), but the gene expression was different in different platelet concentrations.
Conclusion: The results showed that PRP can be effective in POF, but different dosages of PRP can have different effects on the recovery of ovarian.
Objective: This study examined the effect of different platelet concentrations on ovarian function, investigated the apoptosis genes B-cell lymphoma 2 (BCL-2) and Bcl-2-associated X protein, and the differentiation gene octamer-binding transcription factor 4.
Materials and Methods: In this experimental study, 30 female Syrian mice (8-10 wk, 25-30 gr) were randomly divided into 5 groups (n = 6/each): 1) normal control, 2) POF + phosphate buffered saline, 3) POF + PRP 0.25 ml/kg, 4) POF + PRP 0.5 ml/kg, and 5) POF + PRP 1 ml/kg. The cyclophosphamide was used to create a mouse model of POF. After 2 wk from injection of PRP and phosphate buffered saline, ovaries were removed for histological and molecular analysis.
Results: The distribution of different follicle types in the POF + PRP group was the same as that of the control group, according to a morphometric study. Bcl-2-associated X protein gene expression was significantly reduced in the groups that received PRP (p < 0.001). BCL-2 and octamer-binding transcription factor 4 gene expression was also increased in the PRP groups (p < 0.05), but the gene expression was different in different platelet concentrations.
Conclusion: The results showed that PRP can be effective in POF, but different dosages of PRP can have different effects on the recovery of ovarian.
Type of Study: Original Article |
Subject:
Cellular and Molecular Biology of Reproduction
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