یکشنبه 26 بهمن 1404
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دوره 23، شماره 12 - ( 10-1404 )
جلد 23 شماره 12 صفحات 984-971 |
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Vasiee M, Agha-Rahimi A. Complex in vitro fertilization laboratory factors affecting sperm DNA integrity: A narrative review. IJRM 2025; 23 (12) :971-984
URL: http://ijrm.ir/article-1-3743-fa.html
URL: http://ijrm.ir/article-1-3743-fa.html
عوامل پیچیده مؤثر بر تخریب DNA اسپرم در آزمایشگاه: یک مقاله مروری. International Journal of Reproductive BioMedicine. 1404; 23 (12) :971-984
چکیده: (31 مشاهده)
تخریب DNA اسپرم (SDF) D) بهطور فزایندهای بهعنوان شاخص زیستی کلیدی در باروری مردان شناخته میشود و بر باروری طبیعی، نتایج فناوریهای کمکباروری و موفقیت بارداری تأثیر میگذارد. برخلاف پارامترهای متعارف مایع منی،SDF مستقیماً بازتابدهندهی یکپارچگی کروماتین و پتانسیل ژنتیکی اسپرم است. از نظر مکانیسمی، استرس اکسیداتیو و آپوپتوز ناقص، مسیرهای اصلی آسیب DNA بهشمار میآیند. روشهای بالینی و آزمایشگاهی مانند سانتریفیوژ، انکوباسیون طولانیمدت، انجماد و نگهداری نامناسب در دما یا نور نامطلوب میتوانند موجب افزایش گونههای فعال اکسیژن و کاهش پایداری DNA شوند. همچنین مواد مصرفی در آزمایشگاههای فناوری کمکباروری شامل دستکشها، مواد افزودنی محیط کشت و ظروف نگهداری ممکن است ترکیبات سمی آزاد کنند که به DNA اسپرم آسیب میزند. در حالی که برخی روشهای جراحی مانند واریکوسلکتومی کیفیت اسپرم را بهبود میدهند، سایر روشها از جمله برداشت اسپرم از بیضه میتوانند خطر آسیب اکسیداتیو را افزایش دهند. این بررسی روایی از طریق جستجوی جامع متون در پایگاههای داده PubMed، Scopus و Google Scholar برای مطالعات منتشر شده بین سالهای 2000 تا 2025 انجام شد. کلمات کلیدی مرتبط با SDF، استرس اکسیداتیو و عوامل یاتروژنیک در فناوریهای کمک باروری برای مطالعات به زبان انگلیسی استفاده شد. این کلمات غربالگری شدند و دادهها بر اساس ارتباط روششناختی و اهمیت بالینی استخراج شدند. برای کاهش آسیب DNA یاتروژنیک، بهترین شیوهها بر پروتکلهای بهینه جابجایی، محیطهای غنی از آنتیاکسیدان، کنترل کیفیت هوا و روشهای پیشرفته انتخاب اسپرم، مانند میکروفلوئیدیک، تأکید دارند. این بررسی بر عوامل پیچیده و مداخلات آزمایشگاهی که ممکن است ناخواسته SDF را افزایش دهند، تمرکز دارد و استراتژیهای پیشگیرانه را برای حفظ یکپارچگی DNA اسپرم و بهبود نتایج فناوریهای کمکباروری برجسته میکند.
نوع مطالعه: Review article |
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