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Reproductive Biomedicine
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Volume 11, Issue 12 (1-2013)
IJRM 2013, 11(12): 1005-0 |
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Kazemi A, Ramezanzadeh F, Nasr-Esfahani M H, Saboor Yaraghi A A, Ahmadi M. Does dietary fat intake influence oocyte competence and embryo quality by inducing oxidative stress in follicular fluid?. IJRM 2013; 11 (12) :1005-0
URL: http://ijrm.ir/article-1-375-en.html
URL: http://ijrm.ir/article-1-375-en.html
Ashraf Kazemi * 
1, Fatemeh Ramezanzadeh2 , Mohammad Hosein Nasr-Esfahani3 , Ali Akbar Saboor Yaraghi4 , Mehdi Ahmadi5
1, Fatemeh Ramezanzadeh2 , Mohammad Hosein Nasr-Esfahani3 , Ali Akbar Saboor Yaraghi4 , Mehdi Ahmadi5
1- Nursing and Midwifery Care Research Center, Faculty of Nursing and Midwifery, Isfahan University of Medical Sciences, Isfahan, Iran , Kazemi@nm.mui.ac.ir
2- Vali-e-Asr Reproductive Health Research Center, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran
3- Department of Reproduction and Development, Reproductive Biomedicine Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran
4- Department of Nutrition and Biochemistry, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
5- Isfahan Fertility and Infertility Center, Isfahan, Iran
2- Vali-e-Asr Reproductive Health Research Center, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran
3- Department of Reproduction and Development, Reproductive Biomedicine Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran
4- Department of Nutrition and Biochemistry, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
5- Isfahan Fertility and Infertility Center, Isfahan, Iran
Abstract: (3359 Views)
Background: Fat-rich diet may alter oocyte development and maturation and embryonic development by inducing oxidative stress (OS) in follicular environment.
Objective: To investigate the relationship between fat intake and oxidative stress with oocyte competence and embryo quality.
Materials and Methods: In observational study follicular fluid was collected from 236 women undergoing assisted reproduction program. Malon-di-aldehyde (MDA) levels and total antioxidant capacity (TAC) levels of follicular fluid were assessed as oxidative stress biomarkers. In assisted reproduction treatment cycle fat consumption and its component were assessed. A percentage of metaphase ΙΙ stage oocytes, fertilization rate were considered as markers of oocyte competence and non-fragmented embryo rate, mean of blastomer and good cleavage (embryos with more than 5 cells on 3 days post insemination) rate were considered as markers of embryo quality.
Results: The MDA level in follicular fluid was positively related to polyunsaturated fatty acids intake level (p=0.02) and negatively associated with good cleavage rate (p=0.045). Also good cleavage rate (p=0.005) and mean of blastomer (p=0.006) was negatively associated with polyunsaturated fatty acids intake levels. The percentage of metaphase ΙΙ stage oocyte was positively related to the TAC levels in follicular fluid (p=0.046). The relationship between the OS biomarkers in FF and the fertilization rate was not significant.
Conclusion: These findings revealed that fat rich diet may induce the OS in oocyte environment and negatively influence embryonic development. This effect can partially be accounted by polyunsaturated fatty acids uptake while oocyte maturation is related to TAC and oocytes with low total antioxidant capacity have lower chance for fertilization and further development.
Objective: To investigate the relationship between fat intake and oxidative stress with oocyte competence and embryo quality.
Materials and Methods: In observational study follicular fluid was collected from 236 women undergoing assisted reproduction program. Malon-di-aldehyde (MDA) levels and total antioxidant capacity (TAC) levels of follicular fluid were assessed as oxidative stress biomarkers. In assisted reproduction treatment cycle fat consumption and its component were assessed. A percentage of metaphase ΙΙ stage oocytes, fertilization rate were considered as markers of oocyte competence and non-fragmented embryo rate, mean of blastomer and good cleavage (embryos with more than 5 cells on 3 days post insemination) rate were considered as markers of embryo quality.
Results: The MDA level in follicular fluid was positively related to polyunsaturated fatty acids intake level (p=0.02) and negatively associated with good cleavage rate (p=0.045). Also good cleavage rate (p=0.005) and mean of blastomer (p=0.006) was negatively associated with polyunsaturated fatty acids intake levels. The percentage of metaphase ΙΙ stage oocyte was positively related to the TAC levels in follicular fluid (p=0.046). The relationship between the OS biomarkers in FF and the fertilization rate was not significant.
Conclusion: These findings revealed that fat rich diet may induce the OS in oocyte environment and negatively influence embryonic development. This effect can partially be accounted by polyunsaturated fatty acids uptake while oocyte maturation is related to TAC and oocytes with low total antioxidant capacity have lower chance for fertilization and further development.
Keywords: Dietary Fat, Oxidative stress, Follicular fluid, Oocyte competence, Embryo Quality, Malon-de-aldeid, Total antioxidant capacity.
Type of Study: Original Article |
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