Volume 11, Issue 12 (1-2013)                   IJRM 2013, 11(12): 989-0 | Back to browse issues page

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Mohammadi S, Jalali M, Nikravesh M R, Fazel A, Ebrahimzadeh A, Gholamin M et al . Effects of Vitamin-E treatment on CatSper genes expression and sperm quality in the testis of the aging mouse. IJRM 2013; 11 (12) :989-0
URL: http://ijrm.ir/article-1-378-en.html
Effects of Vitamin-E treatment on CatSper genes expression and sperm quality in the testis of the aging mouse
Shabnam Mohammadi1 , Mehdi Jalali * 2, Mohammad Reza Nikravesh1 , Alireza Fazel1 , Alireza Ebrahimzadeh1 , Mehran Gholamin3 , Mojtaba Sankian4
1- Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
2- Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran , JalaliM@mums.ac.ir
3- Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran
4- Bou-ali Research Institute, Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Abstract:   (2874 Views)
Background: CatSper genes are a novel family of four sperm-specific calcium channels, which indicate testis-specific expression patterns. Despite the crucial role of CatSper genes in the male reproduction, very little is known about the factors that regulate their expression.
Objective: The objective of this study was to investigate the effects of vitamin E treatment on the expression of CatSper 1 and CatSper 2 genes as well as sperm quality in the aged male mice.
Materials and Methods: Twenty four 11-12 months old aged male mice and twenty four 2-3-months old young male mice were randomly divided into four groups. Control groups received no injection. The experimental groups of male mice were received intraperitoneal injection of 106 mg/kg vitamin E daily for 35 days. Left testis and cauda epididymides from each mouse were collected on the days 21, 28 and 35 following vitamin E treatment and were used for Real-Time PCR and immunohistochemistry. Also, sperm analysis was performed according to the WHO guidelines given for human sperm examination. Data were analyzed using SPSS software.
Results: Administration of vitamin E improved sperm parameters in the aged as well as young adult male mice. In addition, the expression of CatSper genes increased following vitamin E treatment. Also, intensity of signal for CatSper1 and CatSper2 increased in the head and middle piece of sperm in experimental group as compared to those of control ones.
Conclusion: The vitamin E treatment significantly improved the sperm quality, especially in terms of sperm motility, count and morphology rate. Furthermore, CatSper genes expression could be up-regulated by the vitamin E treatment.
Keywords: CatSper, Sperm, Testis, Mice, Aging, Vitamin E.
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Type of Study: Original Article |
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