Volume 12, Issue 1 (2-2014)                   IJRM 2014, 12(1): 37-46 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Asadi M H, Javanmardi S, Movahedin M. Derivation of ES-like cell from neonatal mouse testis cells in autologous sertoli cells co-culture system. IJRM 2014; 12 (1) :37-46
URL: http://ijrm.ir/article-1-472-en.html
Derivation of ES-like cell from neonatal mouse testis cells in autologous sertoli cells co-culture system
Mohammad Hossein Asadi1 , Setareh Javanmardi * 2, Mansoureh Movahedin3
1- Department of Anatomical Sciences, Faculty of Medical Sciences, Baqiyatallah University of Medical Sciences, Tehran, Iran
2- Department of Anatomical Sciences, Faculty of Medical Sciences, Baqiyatallah University of Medical Sciences, Tehran, Iran , setareh_javanmardy@yahoo.com
3- Department of Anatomical Sciences, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran
Abstract:   (2937 Views)
Background: Spermatogonial stem cell (SSC) is a self-renewing population of male adult stem cell. SSCs have a differentiation potential which are similar to embryonic stem cells. These Embryonic stem like (ES-like) cells can be a potential source for pluripotent cells for stem cell-based therapy.
Objective: This study presents an economical and simple co-culture system for pluripotent stem cells generation from neonatal mouse testis
Materials and Methods: Isolated testicular cells were cultured in DMEM/F12. Characteristics of the isolated cells and obtained ES-like cell were immune-cytochemically confirmed by examining the presence of PLZF, vimentin, Oct4 and Nanog protein. Expression of the pluripotency and germ-cell specific genes was analyzed by qPCR in derived ES-like colony and SSCs respectively.
Results: The experiment results indicated that our method of obtaining pluripotent ES-like cells from spermatogonial cells (SCs) is simpler than the described methods. ES-like cells were immunopositive for pluripotency markers. ES-like cell qPCR results indicated significant increase in pluripotency genes expression and significant decrease in germ cell-specific genes expression.
Conclusion: The results indicated that ES-like cell with pluripotency characteristic were generated from freshly isolated spermatogonial cells. The pluripotent stem cells provide a cellular reservoir usable for regenerative medicine instead of embryonic stem cells. 
Keywords: Spermatogonial stem cells, Embryonic stem like cell, Pluripotent stem cells, Co-culture.
Full-Text [PDF 909 kb]   (555 Downloads) |   |   Full-Text (HTML)  (373 Views)  
Type of Study: Original Article |
References
1. Caires K, Broady J, McLean D. Maintaining the male germline: regulation of spermatogonial stem cells. J Endocrinol 2010; 205: 133-145. [DOI:10.1677/JOE-09-0275]
2. Meistrich ML, Van Beek M. Spermatogonial stem cells: Cell and molecular biology of the testis. New York, Oxford University Press; 1993.
3. de Rooij DG, Russell LD. All you wanted to know about spermatogonia but were afraid to ask. J Androl 2000; 21: 776.
4. Ning L, Goossens E, Geens M, Saen DV, Tournaye H. Spermatogonial stem cells as a source for regenerative medicine. Mid East Fertil Soc J 2012; 17: 1-7. [DOI:10.1016/j.mefs.2011.06.002]
5. Kanatsu-Shinohara M, Lee J, Inoue K, Ogonuki N, Miki H, Toyokuni S, et al. Pluripotency of a single spermatogonial stem cell in mice. Biol Reprod 2008; 78: 681-687. [DOI:10.1095/biolreprod.107.066068]
6. Golestaneh N, Kokkinaki M, Pant D, Jiang J, DeStefano D, Fernandez-Bueno C, et al. Pluripotent stem cells derived from adult human testes. Stem Cells Dev 2009; 18: 1115-1125. [DOI:10.1089/scd.2008.0347]
7. Ning L, Goossens E, Geens M, Van Saen D, Van Riet I, He D, et al. Mouse spermatogonial stem cells obtain morphologic and functional characteristics of hematopoietic cells in vivo. Hum Reprod 2010; 25: 3101-3109. [DOI:10.1093/humrep/deq269]
8. Simon L, Hess RA, Cooke PS. Spermatogonial stem cells, in vivo transdifferentiation and human regenerative medicine. Expert Opin Biol Ther 2010; 10: 519-530. [DOI:10.1517/14712591003614731]
9. Kanatsu-Shinohara M, Inoue K, Lee J, Yoshimoto M, Ogonuki N, Miki H, et al. Generation of pluripotent stem cells from neonatal mouse testis. Cell 2004; 119: 1001-1012. [DOI:10.1016/j.cell.2004.11.011]
10. Simon L, Ekman GC, Kostereva N, Zhang Z, Hess RA, Hofmann MC, et al. Direct transdifferentiation of stem/progenitor spermatogonia into reproductive and nonreproductive tissues of all germ layers. Stem Cells 2009; 27: 1666-1675. [DOI:10.1002/stem.93]
11. Guan K, Nayernia K, Maier LS, Wagner S, Dressel R, Lee JH, et al. Pluripotency of spermatogonial stem cells from adult mouse testis. Nature 2006; 440: 1199-1203. [DOI:10.1038/nature04697]
12. Glaser T, Opitz T, Kischlat T, Konang R, Sasse P, Fleischmann BK, et al. Adult germ line stem cells as a source of functional neurons and glia. Stem Cells 2008; 26: 2434-2443. [DOI:10.1634/stemcells.2008-0163]
13. Izadyar F, Pau F, Marh J, Slepko N, Wang T, Gonzalez R, et al. Generation of multipotent cell lines from a distinct population of male germ line stem cells. Reproduction 2008; 135: 771-784. [DOI:10.1530/REP-07-0479]
14. Seandel M, James D, Shmelkov SV, Falciatori I, Kim J, Chavala S, et al. Generation of functional multipotent adult stem cells from GPR125+ germline progenitors. Nature 2007; 449: 346-350. [DOI:10.1038/nature06129]
15. Im JE, Song SH, Kim JY, Kim KL, Baek SH, Lee DR, et al. Vascular differentiation of multipotent spermatogonial stem cells derived from neonatal mouse testis. Exp Mol Med 2012; 44: 303-309. [DOI:10.3858/emm.2012.44.4.034]
16. Kanatsu-Shinohara M, Ogonuki N, Inoue K, Miki H, Ogura A, Toyokuni S, et al. Long-term proliferation in culture and germline transmission of mouse male germline stem cells. Biol Reprod 2003; 69: 612-616. [DOI:10.1095/biolreprod.103.017012]
17. Kubota H, Avarbock MR, Brinster RL. Growth factors essential for self-renewal and expansion of mouse spermatogonial stem cells. Proc Natl Acad Sci USA 2004; 101: 16489-16494. [DOI:10.1073/pnas.0407063101]
18. Meng X, Lindahl M, Hyvönen ME, Parvinen M, de Rooij DG, Hess MW, et al. Regulation of cell fate decision of undifferentiated spermatogonia by GDNF. Science 2000; 287: 1489-1493. [DOI:10.1126/science.287.5457.1489]
19. Naughton CK, Jain S, Strickland AM, Gupta A, Milbrandt J. Glial cell-line derived neurotrophic factor-mediated RET signaling regulates spermatogonial stem cell fate. Biol Reprod 2006; 74: 314-321. [DOI:10.1095/biolreprod.105.047365]
20. Hess R, França LR, Skinner M, Griswold M. Structure of the Sertoli cell. In: Skinner MK, Grinswold MD (Eds). Sertoli Cell Biology. California, Elsevier Academic Press; 2005: 19-40. [DOI:10.1016/B978-012647751-1/50004-0]
21. Huleihel M, Lunenfeld E. Regulation of spermatogenesis by paracrine/autocrine testicular factors. Asian J Androl 2004; 6: 259-268.
22. Parks J, Lee D, Huang S, Kaproth M. Prospects for spermatogenesis in vitro. Theriogenology 2003; 59: 73-86. [DOI:10.1016/S0093-691X(02)01275-X]
23. Roser JF. Regulation of testicular function in the stallion: an intricate network of endocrine, paracrine and autocrine systems. Anim Reprod Sci 2008; 107: 179-196. [DOI:10.1016/j.anireprosci.2008.05.004]
24. Merhi RA, Guillaud L, Delouis C, Cotinot C. Establishment and characterization of immortalized ovine Sertoli cell lines. In Vitro Cell Dev Biol Anim 2001; 37: 581-588. https://doi.org/10.1290/1071-2690(2001)037<0581:EACOIO>2.0.CO;2 [DOI:10.1290/1071-2690(2001)0372.0.CO;2]
25. Pognan F, Masson MT, Lagelle F, Charuel C. Establishment of a rat Sertoli cell line that displays the morphological and some of the functional characteristics of the native cell. Cell Biol Toxicol 1997; 13: 453-463. [DOI:10.1023/A:1007475928452]
26. Zhang D, He D, Wei G, Song XF, Li XL, In T. Long-term Coxculture of Spermatogonial Stem Cells on Sertoli Cells Feeder Layer in vitro. Sichuan Da Xue Xue Bao Yi Xue Ban 2008; 39: 6.
27. Liu S, Tang Z, Xiong T, Tang W. Isolation and characterization of human spermatogonial stem cells. Reprod Biol Endocrinol 2011; 9: 141. [DOI:10.1186/1477-7827-9-141]
28. Mohamadi S, Movahedin M, Koruji S, Jafarabadi MA, Makoolati Z. Comparison of colony formation in adult mouse spermatogonial stem cells developed in Sertoli and STO coculture systems. Andrologia 2011; 44: 431-437. [DOI:10.1111/j.1439-0272.2011.01201.x]
29. Simon L, Hofmann MC, Cooke PS. Spermatogonial Stem Cells: An Alternate Source of Pluripotent Stem Cells for Regenerative Medicine. Expert Opin Biol Ther 2010; 10: 519-530. [DOI:10.1517/14712591003614731]
30. Nagano M, Brinster CJ, Orwig KE, Ryu BY, Avarbock MR, Brinster RL. Transgenic mice produced by retroviral transduction of male germ-line stem cells. Proc Natl Acad Sci USA 2001; 98: 13090-13095. [DOI:10.1073/pnas.231473498]
31. Ryu BY, Kubota H, Avarbock MR, Brinster RL. Conservation of spermatogonial stem cell self-renewal signaling between mouse and rat. Proc Natl Acad Sci USA 2005; 102: 14302-14307. [DOI:10.1073/pnas.0506970102]
32. Nagano MC, Yeh JR. The Cluster-Forming Activity Assay: A Short-Term In Vitro Method to Analyze the Activity of Mouse Spermatogonial Stem Cells. In: Orwing KE, Hermann BP (Eds). Male Germline Stem Cells: Developmental and Regenerative Potential. New York, Humana Press; 2011: 125-134. [DOI:10.1007/978-1-61737-973-4_6]
33. Kossack N, Meneses J, Shefi S, Nguyen HN, Chavez S, Nicholas C, et al. Isolation and Characterization of Pluripotent Human Spermatogonial Stem Cell‐Derived Cells. Stem Cells 2009; 27: 138-149. [DOI:10.1634/stemcells.2008-0439]
34. Hamra FK, Chapman KM, Nguyen DM, Williams-Stephens AA, Hammer RE, Garbers DL. Self renewal, expansion, and transfection of rat spermatogonial stem cells in culture. Proc Natl Acad Sci USA 2005; 102: 17430-17435. [DOI:10.1073/pnas.0508780102]
35. Tadokoro Y, Yomogida K, Ohta H, Tohda A, Nishimune Y. Homeostatic regulation of germinal stem cell proliferation by the GDNF/FSH pathway. Mech Dev 2002; 113: 29-39. [DOI:10.1016/S0925-4773(02)00004-7]
36. Kurita K, Sakai N. Functionally distinctive testicular cell lines of zebrafish to support male germ cell development. Mol Reprod Dev 2004; 67: 430-438. [DOI:10.1002/mrd.20035]
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb