Volume 12, Issue 1 (2-2014)                   IJRM 2014, 12(1): 29-36 | Back to browse issues page

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Cong Y, Cui L, Zhang Z, Xi J, Wang M. Rabbit antiserum to mouse embryonic stem cells delays compaction of mouse preimplantation embryos. IJRM 2014; 12 (1) :29-36
URL: http://ijrm.ir/article-1-479-en.html
Rabbit antiserum to mouse embryonic stem cells delays compaction of mouse preimplantation embryos
Yingli Cong1 , Lifang Cui2 , Zhenhong Zhang2 , Jianzhong Xi2 , Mianjuan Wang * 3
1- College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, Gansu 730020, China
2- College of Animal Science and Technology, Agricultural University of Hebei, Baoding, Hebei 071001, China
3- School Hospital of Agricultural University of Hebei, Baoding, Hebei 071001, China , Wangmianjuan@aliyun.com
Abstract:   (2606 Views)
Background: Mouse embryonic stem (ES) cells are derived from the inner cell mass (ICM) of the preimplantation blastocysts. So it is suggested that ES and ICM cells should have similar cellular surface molecules and antiserum to ES cells can inhibit ICM development.
Objective: The objective of this study was to evaluate the effect of rabbit antiserum to ES cells on mouse preimplantation embryo development and chimera production.
Materials and Methods: Mouse 4-cell embryos were matured in vitro at 37.5oC, in humidified 5% CO2 atmosphere for 12-36 h. The embryos were cultured in KSOM medium with or without antiserum for 12-36 h. The ratios of in vitro embryo development of the blastocysts, cell division, attachment potential, alkaline phosphatase activity, post-implantation development, and chimera production were assessed and compared with the control group. P<0.05 was considered as significant.
Results: The rabbit antiserum to mouse ES cells showed delay in embryo compaction and induced decompaction at 8-cell stage. The development of 4-cell embryos in the presence of the antiserum for 36h did not lead to a reduced or absent ICM. These embryos still displayed positive alkaline phosphatase activity, normal cell division, embryo attachment, outgrowth formation, implantation and post-implantation development. In addition, decompaction induced by antiserum did not increase production and germline transmission of chimeric mice.
Conclusion: The results showed that antiserum to ES cells delayed embryo compaction and did not affect post-implantation development and chimera production.
Keywords: Mouse, Embryonic stem cells, Immune sera, Preimplantation development, Chimera.
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Type of Study: Original Article |
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