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Volume 5, Issue 4 (7-2007)
IJRM 2007, 5(4): 89-93 |
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Mohammadzadeh A, Heidari M, Soltan Ghoraiee H, Ghaffari Novin M, Jeddi-Tehrani M, Akhondi M M, et al . The effect of pentoxifylline on the growth of endometrial implants and leukocytes in rats.. IJRM 2007; 5 (4) :89-93
URL: http://ijrm.ir/article-1-82-en.html
URL: http://ijrm.ir/article-1-82-en.html
Afsaneh Mohammadzadeh * 
1, Mahnaz Heidari2 , Haleh Soltan Ghoraiee3 , Marefat Ghaffari Novin4 , Mahmood Jeddi-Tehrani5 , Mohammad Mahdi Akhondi5 , Hojjat Zeraati5 , Farzaneh Mohammadzadeh6 , Pegah Ebadi4
1, Mahnaz Heidari2 , Haleh Soltan Ghoraiee3 , Marefat Ghaffari Novin4 , Mahmood Jeddi-Tehrani5 , Mohammad Mahdi Akhondi5 , Hojjat Zeraati5 , Farzaneh Mohammadzadeh6 , Pegah Ebadi4
1- Department of Reproductive Endocrinology and Embryology, Reproductive Biotechnology Research Center, Avesina Research Institute, ACECR, Tehran, Iran , af23mohammadzadeh@yahoo.com
2- Departmet of Nanotechnology , Nanobiotechnology Research Center , Avesina Research Institute, ACECR, Tehran, Iran
3- Department of Reproductive and Biotechnology Genetic, Reproductive Biotechnology Research Center, Avesina Research Institute ,ACECR, Tehran, Iran
4- Department of Reproductive Endocrinology and Embryology, Reproductive Biotechnology Research Center, Avesina Research Institute, ACECR, Tehran, Iran
5- Department of Monoclonal Antibody, Monoclonal Antibody Research Center , Avesina Research Institute ,ACERCR, Tehran, Iran
6- Anatomy Department, Iran University of Medical Science, Tehran, Iran
2- Departmet of Nanotechnology , Nanobiotechnology Research Center , Avesina Research Institute, ACECR, Tehran, Iran
3- Department of Reproductive and Biotechnology Genetic, Reproductive Biotechnology Research Center, Avesina Research Institute ,ACECR, Tehran, Iran
4- Department of Reproductive Endocrinology and Embryology, Reproductive Biotechnology Research Center, Avesina Research Institute, ACECR, Tehran, Iran
5- Department of Monoclonal Antibody, Monoclonal Antibody Research Center , Avesina Research Institute ,ACERCR, Tehran, Iran
6- Anatomy Department, Iran University of Medical Science, Tehran, Iran
Abstract: (3191 Views)
Background: Immune system disturbances have an important role in endometriosis which may lead to infertility. It seems that inflammatory cytokines specially tumor necrosis factor alpha (TNF-a) which were produced by activated macrophages have an important role in pathology of endometriosis. Based on this theory, anti TNF-a drugs like pentoxifylline (PX) are suggested as new drugs for Endometriosis.
Objective: This experimental study has been done on female rats to determine the effect of PX on the endometrial implants and leukocytes in serum.
Material and Methods: In proestrous phase, one horn of rat’s bicorn uterus was removed surgically and the endometrium was implanted to different places as follows: subcutaneous, peritoneum and near ovaries. After two months observation, female rats divided into two groups randomly. In treated group (n=10) PX (5mg/kg twice a day) and in control group (n=10), normal saline (same dose) were injected subcutaneously. Then, via second laparotomy and in the same phase of the cycles, the size of implants and the amount of leukocytes in serum were measured.
Results: In treated group compared with control, the size of implants was decreased significantly in right subcutaneous (8.05mm vs 13.50mm) p<0.01, left subcutaneous (7.64 mm vs 14mm) p<0.01, right ovary (6.64 mm vs 15.22mm) p<0.001 and left ovary (7.18 mm vs 14.56 mm) p<0.005. In treated group, the total leukocyte count (5259.54 ± 178.78 vs 15833.33 ± 259.27) p<0.02 was decreased. The number of esterous cycle was similar in both groups.
Conclusion: PX can reduce the size of endometrial implants as well as leukocyte count.
Objective: This experimental study has been done on female rats to determine the effect of PX on the endometrial implants and leukocytes in serum.
Material and Methods: In proestrous phase, one horn of rat’s bicorn uterus was removed surgically and the endometrium was implanted to different places as follows: subcutaneous, peritoneum and near ovaries. After two months observation, female rats divided into two groups randomly. In treated group (n=10) PX (5mg/kg twice a day) and in control group (n=10), normal saline (same dose) were injected subcutaneously. Then, via second laparotomy and in the same phase of the cycles, the size of implants and the amount of leukocytes in serum were measured.
Results: In treated group compared with control, the size of implants was decreased significantly in right subcutaneous (8.05mm vs 13.50mm) p<0.01, left subcutaneous (7.64 mm vs 14mm) p<0.01, right ovary (6.64 mm vs 15.22mm) p<0.001 and left ovary (7.18 mm vs 14.56 mm) p<0.005. In treated group, the total leukocyte count (5259.54 ± 178.78 vs 15833.33 ± 259.27) p<0.02 was decreased. The number of esterous cycle was similar in both groups.
Conclusion: PX can reduce the size of endometrial implants as well as leukocyte count.
Type of Study: Original Article |
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